Results 131 to 140 of about 60,573 (210)

Metal-Based Drug-DNA Interactions and Analytical Determination Methods. [PDF]

open access: yesMolecules
Hangan AC   +5 more
europepmc   +1 more source

Thermal denaturation of nucleic acids in polyacrylamide gels

open access: closedAnalytical Biochemistry, 1974
Abstract A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies.
Arie Rosner   +2 more
openalex   +4 more sources

Deoxypentose nucleic acids XIV. The denaturation of deoxyribonucleic acid in aqueous solution: properties of DNA denaturated by dilution

open access: closedBiochimica et Biophysica Acta, 1960
Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
Ross B. Inman, D. O. Jordan
openalex   +4 more sources

Small-volume cuvette for high-resolution thermal denaturation of nucleic acids

open access: closedAnalytical Biochemistry, 1979
Abstract A small-volume cuvette and hoider makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 μg of DNA. The reduced sample size is especially important in the thermal characterization of DNA restriction fragments.
Allen T. Ansevin, Douglas L. Vizard
openalex   +4 more sources

Numerical analysis of thermal denaturation of nucleic acids

open access: closedAnalytical Biochemistry, 1978
Denaturation of DNA molecules by stepwise incrementation of the temperature leads to melting profiles showing a fine structure, composed of individual melting modes. A method is described by which quantitative physical information brought by the modes can be extracted from the melting profile.
J. Gabarro
openalex   +4 more sources

Effects of cupric ions on thermal denaturation of nucleic acids

open access: closedJournal of Molecular Biology, 1965
The midpoint of thermal denaturation of sodium DNA and RNA decreases with increasing concentration of cupric ions. With Micrococcus lysodeikticus DNA, the Tm† is 69°C without cupric ions and 36°C with 0·1 m M -cupric ions in 5 m M -sodium chloride. In a solvent consisting of 5 m M -sodium chloride and 0·1 m M -cupric ions, Tm values of several DNA's ...
Susumu Hiai
openalex   +4 more sources

Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins

open access: closed, 2000
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D. Jeremy Williams, Kathleen B. Hall
openalex   +4 more sources

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