Results 131 to 140 of about 60,573 (210)
Visual Detection of Canine Monocytic Ehrlichiosis Using Polymerase Chain Reaction-Based Lateral Flow Biosensors. [PDF]
Sumpavong P+2 more
europepmc +1 more source
Metal-Based Drug-DNA Interactions and Analytical Determination Methods. [PDF]
Hangan AC+5 more
europepmc +1 more source
Thermal denaturation of nucleic acids in polyacrylamide gels
Abstract A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies.
Arie Rosner+2 more
openalex +4 more sources
Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
Ross B. Inman, D. O. Jordan
openalex +4 more sources
Small-volume cuvette for high-resolution thermal denaturation of nucleic acids
Abstract A small-volume cuvette and hoider makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 μg of DNA. The reduced sample size is especially important in the thermal characterization of DNA restriction fragments.
Allen T. Ansevin, Douglas L. Vizard
openalex +4 more sources
Numerical analysis of thermal denaturation of nucleic acids
Denaturation of DNA molecules by stepwise incrementation of the temperature leads to melting profiles showing a fine structure, composed of individual melting modes. A method is described by which quantitative physical information brought by the modes can be extracted from the melting profile.
J. Gabarro
openalex +4 more sources
Effects of cupric ions on thermal denaturation of nucleic acids
The midpoint of thermal denaturation of sodium DNA and RNA decreases with increasing concentration of cupric ions. With Micrococcus lysodeikticus DNA, the Tm† is 69°C without cupric ions and 36°C with 0·1 m M -cupric ions in 5 m M -sodium chloride. In a solvent consisting of 5 m M -sodium chloride and 0·1 m M -cupric ions, Tm values of several DNA's ...
Susumu Hiai
openalex +4 more sources
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D. Jeremy Williams, Kathleen B. Hall
openalex +4 more sources