Results 51 to 60 of about 323,426 (336)

Locked Nucleic Acid Oligonucleotides Facilitate RNA•LNA-RNA Triple-Helix Formation and Reduce MALAT1 Levels

International Journal of Molecular Sciences
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and multiple endocrine neoplasia-β (MENβ) are two long noncoding RNAs upregulated in multiple cancers, marking these RNAs as therapeutic targets.
Krishna M Shivakumar, Gowthami Mahendran, Jessica A. Brown   +2 more
semanticscholar   +1 more source

Peptide Nucleic Acid Conjugated with Ruthenium‐Complex Stabilizing Double‐Duplex Invasion Complex Even under Physiological Conditions

ChemBioChem, 2018
Peptide nucleic acid (PNA) can form a stable duplex with DNA, and, accordingly, directly recognize double‐stranded DNA through the formation of a double‐duplex invasion complex, wherein a pair of complementary PNA strands form two PNA/DNA duplexes ...
Masaki Hibino, Yuichiro Aiba, Y. Watanabe, O. Shoji   +3 more
semanticscholar   +1 more source

Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: implications for DNA hybridization

, 2011
Loops are essential secondary structure elements in folded DNA and RNA molecules and proliferate close to the melting transition. Using a theory for nucleic acid secondary structures that accounts for the logarithmic entropy c ln m for a loop of length m,
Einert, Thomas R., Netz, Roland R., Orland, Henri   +2 more
core   +1 more source

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

Journal of Functional Biomaterials, 2012
Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using ...
R. Maeda, T. Isoda
doaj   +1 more source

A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. [PDF]

PLoS ONE, 2015
Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries.
Grace Wong, Isaac Wong, Kamfai Chan, Yicheng Hsieh, Season Wong   +4 more
doaj   +1 more source

Novel isoguanine derivative of unlocked nucleic acid—Investigations of thermodynamics and biological potential of modified thrombin binding aptamer

PLoS ONE, 2018
Thrombin binding aptamer (TBA), is a short DNA 15-mer that forms G-quadruplex structure and possesses anticoagulant properties. Some chemical modifications, including unlocked nucleic acids (UNA), 2′-deoxy-isoguanosine and 2′-deoxy-4-thiouridine were ...
Weronika Kotkowiak, Tomasz Czapik, A. Pasternak   +2 more
semanticscholar   +1 more source

Twist versus Nonlinear Stacking in Short DNA Molecules

, 2014
The denaturation of the double helix is a template for fundamental biological functions such as replication and transcription involving the formation of local fluctuational openings.
Zoli, Marco
core   +1 more source

Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation. [PDF]

PLoS ONE, 2016
Human immunodeficiency virus (HIV) is a chronic infection that can be managed by antiretroviral treatment (ART). However, periods of suboptimal viral suppression during lifelong ART can select for HIV drug resistant (DR) variants.
Nuttada Panpradist, Ingrid A Beck, Michael H Chung, James N Kiarie, Lisa M Frenkel, Barry R Lutz   +5 more
doaj   +1 more source

Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus.

PLoS ONE, 2020
Molecular-based testing of poultry dust has been used as a fast, sensitive and specific way to monitor viruses in chicken flocks but it provides no information on viral viability.
Yugal Raj Bindari, Stephen W Walkden-Brown, Priscilla F Gerber   +2 more
doaj   +1 more source

Hypoxanthine-Guanine Phosphoribosyltransferase/adenylate Kinase From Zobellia galactanivorans: A Bifunctional Catalyst for the Synthesis of Nucleoside-5′-Mono-, Di- and Triphosphates

Frontiers in Bioengineering and Biotechnology, 2020
In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT ...
Javier Acosta, Jon Del Arco, Maria Luisa Del Pozo, Beliña Herrera-Tapias, Vicente Javier Clemente-Suárez, Vicente Javier Clemente-Suárez, José Berenguer, Aurelio Hidalgo, Jesús Fernández-Lucas, Jesús Fernández-Lucas   +9 more
doaj   +1 more source