Sequences for differential diagnostic of Ehrlichia ruminantium and use thereof [PDF]
The invention provides genes that are unique either to Ehrlichia ruminantium strain Gardel or to Ehrlichia ruminantium strain Welgevonden, or allelic couples which are present in both strains but whose sequences differ between the two strains, as genetic
Demaille, Jacques+3 more
core
Computer programs to assist in high resolution thermal denaturation and circular dichroism studies on nucleic acids [PDF]
Thomas C. Goodman+3 more
openalex +1 more source
Using electropolymerization based doping for electro-addressable functionalization of a multi-electrode array probe for nucleic acid detection [PDF]
Here, we report a facile method for electro-addressable functionalization of a probe comprising of closely spaced three individually addressable carbon fiber electrodes for detection of nucleic acids. First, a multi electrode array probe comprising three adjacent carbon fiber electrodes was fabricated through pulling a three-barrel glass capillary with
arxiv
Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO. [PDF]
dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA molecules that have affected studies of immune function
Delwart, Eric+2 more
core
Recent applicable delivery approaches of peptide nucleic acids to the target cells [PDF]
Peptide nucleic acids (PNAs) are artificial nucleic acids with a peptide backbone instead of sugar phosphate backbone of DNA or RNA. Their resistance to degradation, selectivity and greater binding affinity in comparison to usual nucleic acids led to consideration of their great potential for different applications.
arxiv
Modeling the thermal evolution of enzyme-created bubbles in DNA
The formation of bubbles in nucleic acids (NAs) are fundamental in many biological processes such as DNA replication, recombination, telomeres formation, nucleotide excision repair, as well as RNA transcription and splicing.
Archilla, J. F. R.+2 more
core +2 more sources
Denaturation of proteins and nucleic acids by thermal-gradient electrophoresis [PDF]
D R Thatcher, B A Hodson
openalex +1 more source
Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction [PDF]
Gerhard Steger
openalex +1 more source
Torsion and bending of nucleic acids studied by subnanosecond time-resolved fluorescence depolarization of intercalated dyes [PDF]
Subnanosecond time‐resolved fluorescence depolarization has been used to monitor the reorientation of ethidium bromide intercalated in native DNA, synthetic polynucleotide complexes, and in supercoiled plasmid DNA.
Millar, D. P.+2 more
core
The Reverse Transcription Signature of N-\u3csub\u3e1\u3c/sub\u3e-Methyladenosine in RNA-Seq is Sequence Dependent [PDF]
The combination of Reverse Transcription (RT) and high-throughput sequencing has emerged as a powerful combination to detect modified nucleotides in RNA via analysis of either abortive RT-products or of the incorporation of mismatched dNTPs into cDNA ...
Alfonzo, Juan+14 more
core +1 more source