Results 121 to 130 of about 6,739 (182)

An improved method for the isolation of some nucleoside diphosphate sugars from yeast

open access: closedBiochimica et Biophysica Acta, 1957
Abstract An improved method is described for the simultaneous isolation of UDPG, UDPAG and GDPM from yeast. The method involves extraction with ethanol and chromatography on anion exchange resin columns. The nucleotides are obtained from the effluent by adsorption on charcoal and subsequent elution with aqueous ethanol. A washing of the charcoal with
H G, PONTIS, E, CABIB, L F, LELOIR
openaire   +3 more sources

Ion-exchange paper chromatography of nucleoside diphosphate sugars and related nucleotides

open access: closedAnalytical Biochemistry, 1965
Abstract A new method especially adapted for the characterization of nucleoside diphosphate sugars in mixtures containing nucleoside mono-, di-, and tri-phosphates is described. It is based on ion-exchange chromatography using papers impregnated with polyethyleneimine (PEI).
H, Verachtert   +3 more
openaire   +3 more sources

The Mechanism of Phosphorylation of Natural Nucleosides and Anti-HIV Analogues by Nucleoside Diphosphate Kinase Is Independent of Their Sugar Substituents

open access: closedChemBioChem, 2002
The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system on the basis of various X-ray crystallographic structures.
Michael C, Hutter, Volkhard, Helms
  +6 more sources

Regulation of the Synthesis of Nucleoside Diphosphate Sugars in Reticulo-Endothelial Tissues

open access: closedEuropean Journal of Biochemistry, 1975
The kinetic and regulatory properties of enzymes involved in the biosynthesis of UDP-D-galactose, UDP-N-acetylglucosamine. GDP-alpha-D-mannose and GDP-beta-L-fucose from D-glucose 6-phosphate in various reticulo-endothelial tissues was studied. The tissues examined include bovine liver, thyroid, spleen, salivary gland, lung, intestine and mesenteric ...
J, Mendicino, A K, Rao
openaire   +3 more sources

An improved P(V)-N activation strategy for the synthesis of nucleoside diphosphate 6-deoxy-l-sugars

open access: closedTetrahedron, 2014
Abstract Four nucleoside diphosphate 6-deoxy- l -sugars have been efficiently synthesized by coupling sugar-1-phosphates prepared from the H-phosphonate precursors with nucleoside 5′-phosphoropiperidates in the presence of 4,5-dicyanoimidazole (DCI) as the activator. Compared to the conventional 1H-tetrazole-promoted phosphoromorpholidate method, the
Qi Sun   +5 more
  +4 more sources

[9] The separation of nucleoside diphosphate sugars and related nucleotides by ion-exchange paper chormatography

open access: closed, 1966
Publisher Summary This chapter discusses the ion-exchange paper chromatography method for the separation of nucleoside diphosphate sugars and related nucleotides. By combining the rapidity and discrimination of ionexchange resins with the sensitivity of filter paper as a supporting medium, a rapid and efficient separation of small quantities of the ...
H. Verachtert   +3 more
openaire   +2 more sources

Chemical and enzymic degradations of nucleoside mono- and diphosphate sugars

open access: closedBiochimica et Biophysica Acta (BBA) - General Subjects, 1979
G. Spik, P. Six, J. Montreuil
openaire   +2 more sources

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