Results 121 to 130 of about 6,739 (182)
An improved method for the isolation of some nucleoside diphosphate sugars from yeast
Abstract An improved method is described for the simultaneous isolation of UDPG, UDPAG and GDPM from yeast. The method involves extraction with ethanol and chromatography on anion exchange resin columns. The nucleotides are obtained from the effluent by adsorption on charcoal and subsequent elution with aqueous ethanol. A washing of the charcoal with
H G, PONTIS, E, CABIB, L F, LELOIR
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Ion-exchange paper chromatography of nucleoside diphosphate sugars and related nucleotides
Abstract A new method especially adapted for the characterization of nucleoside diphosphate sugars in mixtures containing nucleoside mono-, di-, and tri-phosphates is described. It is based on ion-exchange chromatography using papers impregnated with polyethyleneimine (PEI).
H, Verachtert +3 more
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The reaction mechanism of the phosphoryl transfer catalyzed by dinucleoside diphosphate kinase from Dictyostelium discoideum is investigated by semiempirical AM1 molecular orbital computation of an active site model system on the basis of various X-ray crystallographic structures.
Michael C, Hutter, Volkhard, Helms
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Regulation of the Synthesis of Nucleoside Diphosphate Sugars in Reticulo-Endothelial Tissues
The kinetic and regulatory properties of enzymes involved in the biosynthesis of UDP-D-galactose, UDP-N-acetylglucosamine. GDP-alpha-D-mannose and GDP-beta-L-fucose from D-glucose 6-phosphate in various reticulo-endothelial tissues was studied. The tissues examined include bovine liver, thyroid, spleen, salivary gland, lung, intestine and mesenteric ...
J, Mendicino, A K, Rao
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Periplasmic nucleoside diphosphate sugar hydrolase from Bacillus subtilis
J, Mauck, L, Glaser
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An improved P(V)-N activation strategy for the synthesis of nucleoside diphosphate 6-deoxy-l-sugars
Abstract Four nucleoside diphosphate 6-deoxy- l -sugars have been efficiently synthesized by coupling sugar-1-phosphates prepared from the H-phosphonate precursors with nucleoside 5′-phosphoropiperidates in the presence of 4,5-dicyanoimidazole (DCI) as the activator. Compared to the conventional 1H-tetrazole-promoted phosphoromorpholidate method, the
Qi Sun +5 more
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Publisher Summary This chapter discusses the ion-exchange paper chromatography method for the separation of nucleoside diphosphate sugars and related nucleotides. By combining the rapidity and discrimination of ionexchange resins with the sensitivity of filter paper as a supporting medium, a rapid and efficient separation of small quantities of the ...
H. Verachtert +3 more
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Chemical and enzymic degradations of nucleoside mono- and diphosphate sugars
G. Spik, P. Six, J. Montreuil
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Sugar Nucleoside Diphosphates via a Reactive Zwitterionic Phosphoramidate Intermediate
C. E. McKenna +2 more
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THE ROLE OF PYRIDINE NUCLEOTIDES IN INTERCONVERSIONS OF NUCLEOSIDE DIPHOSPHATE SUGARS
David S. Feingold
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