Results 51 to 60 of about 104,610 (371)
Precise genome-wide mapping of single nucleosomes and linkers in vivo
Genome Biology, 2018 We developed a chemical cleavage method that releases single nucleosome dyad-containing fragments, allowing us to precisely map both single nucleosomes and linkers with high accuracy genome-wide in yeast.
Răzvan V. Chereji +3 more
semanticscholar +1 more source
ISRN Molecular Biology, 2012 Nucleosome positioning is not only related to genomic DNA compaction but also to other biological functions. After the chromatin is digested by micrococcal nuclease, nucleosomal (nucleosome-bound) DNA fragments can be sequenced and mapped on the genomic DNA sequence.
openaire +2 more sources
Mediator–Nucleosome Interaction [PDF]
Molecular Cell, 2000 Mediator, a multiprotein complex involved in the regulation of RNA polymerase II transcription, binds to nucleosomes and acetylates histones. Three lines of evidence identify the Nut1 subunit of Mediator as responsible for the histone acetyltransferase (HAT) activity. An "in-gel" HAT assay reveals a single band of the appropriate size.
Lawrence C. Myers +4 more
openaire +3 more sources
High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier. [PDF]
, 2019 Nucleosomes represent mechanical and energetic barriers that RNA Polymerase II (Pol II) must overcome during transcription. A high-resolution description of the barrier topography, its modulation by epigenetic modifications, and their effects on Pol II ...
Brown, Aidan I +9 more
core +2 more sources
eLife, 2019 Tardigrades, also known as water bears, are animals that can survive extreme conditions. The tardigrade Ramazzottius varieornatus contains a unique nuclear protein termed Dsup, for damage suppressor, which can increase the resistance of human cells to ...
C. Chavez +4 more
semanticscholar +1 more source
Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation
eLife, 2021 In meiosis, DNA double-strand break (DSB) formation by Spo11 initiates recombination and enables chromosome segregation. Numerous factors are required for Spo11 activity, and couple the DSB machinery to the development of a meiosis-specific ‘axis ...
Dorota Rousová +10 more
doaj +1 more source
Nucleosomes affect local transformation efficiency [PDF]
, 2012 Genetic transformation is a natural process during which foreign DNA enters a cell and integrates into the genome. Apart from its relevance for horizontal gene transfer in nature, transformation is also the cornerstone of today's recombinant gene ...
Aslankoohi, Elham +6 more
core +2 more sources
DNA damage detection in nucleosomes involves DNA register shifting
Nature, 2019 Access to DNA packaged in nucleosomes is critical for gene regulation, DNA replication and DNA repair. In humans, the UV-damaged DNA-binding protein (UV-DDB) complex detects UV-light-induced pyrimidine dimers throughout the genome; however, it remains ...
Syota Matsumoto +12 more
semanticscholar +1 more source
Nucleosomes in pancreatic cancer patients during radiochemotherapy [PDF]
, 2005 Nucleosomes appear spontaneously in elevated concentrations in the serum of patients with malignant diseases as well as during chemo- and radiotherapy. We analyzed whether their kinetics show typical characteristics during radiochemotherapy and enable an
Amoura Z +19 more
core +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 2019 Significance Nucleosomes are the base units that organize eukaryotic genomes. Besides the canonical histone, histone variants create unique local chromatin domains that fine-tune transcription, replication, DNA damage repair, and faithful chromosome ...
Daniël P. Melters +6 more
semanticscholar +1 more source