Results 61 to 70 of about 72,215 (324)

VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

eLife, 2018
VivosX is an in vivo disulfide crosslinking approach that utilizes a pair of strategically positioned cysteines on two proteins to probe physical interactions within cells.
Chitra Mohan, Lisa M Kim, Nicole Hollar, Tailai Li, Eric Paulissen, Cheuk T Leung, Ed Luk   +6 more
doaj   +1 more source

Electrophoresis of Positioned Nucleosomes [PDF]

Biophysical Journal, 2007
to appear in Biophys. J.
Martin Castelnovo, Sebastian Grauwin
openaire   +4 more sources

Hyperoxia Induced Alteration of Chromatin Structure in Human Bone Marrow Derived Primary Mesenchymal Stromal Cells

Advanced Biology, EarlyView.
Chromatin, which organizes DNA, changes its structure to adapt to stress like high oxygen levels (hyperoxia), which can damage cells. Researchers developed a technique to observe these changes and found variability in how different parts of chromatin remodel.
Lauren Monroe, Samantha Kaonis, Natalie Calahan, Neda Kabi, Soham Ghosh   +4 more
wiley   +1 more source

Nucleosome destabilization by polyamines

Archives of Biochemistry and Biophysics, 2022
The roles and molecular interactions of polyamines (PAs) in the nucleus are not fully understood. Here their effect on nucleosome stability, a key regulatory factor in eukaryotic gene control, is reported, as measured in agarose embedded nuclei of H2B-GFP expressor HeLa cells.
Laszlo Imre, Erfaneh Firouzi Niaki, Rosevalentine Bosire, Peter Nanasi, Peter Nagy, Zsolt Bacso, Nubar Hamidova, Yves Pommier, Albert Jordan, Gabor Szabo   +9 more
openaire   +3 more sources

Topoisomerase I Inhibition in ETV4‐overexpressed Non‐Small Cell Lung Cancer Promotes Replication and Transcription Mediated R‐Loop Accumulation and DNA Damage

Advanced Science, EarlyView.
The oncogenic ETS factor ETV4 exerts a pleiotropic control over DNA replication both in a transcription‐dependent and ‐independent fashion in NSCLC cells. High‐ETV4 expression leads to R‐loop formation and DNA damage in response to TOP1 inhibition.
Jiaxi Zhang, Yan Wang, Shanhu Cao, Shelly M. Xie, Bei Liu, Yimeng Li, Yuqi Hou, Xue Meng, Mingzhu Ruan, Di Bu, Jia Kang, Ruxin Li, Lei Lou, Juan Wang, Lingxiao Xing   +14 more
wiley   +1 more source

Transcriptionally Driven DNA Replication Program of the Human Parasite Leishmania major

Cell Reports, 2016
Faithful inheritance of eukaryotic genomes requires the orchestrated activation of multiple DNA replication origins (ORIs). Although origin firing is mechanistically conserved, how origins are specified and selected for activation varies across different
Rodrigo Lombraña, Alba Álvarez, José Miguel Fernández-Justel, Ricardo Almeida, César Poza-Carrión, Fábia Gomes, Arturo Calzada, José María Requena, María Gómez   +8 more
doaj   +1 more source

Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells. [PDF]

PLoS Biology, 2007
Centromeres, the specialized chromatin structures that are responsible for equal segregation of chromosomes at mitosis, are epigenetically maintained by a centromere-specific histone H3 variant (CenH3).
Yamini Dalal, Hongda Wang, Stuart Lindsay, Steven Henikoff   +3 more
doaj   +1 more source

The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors

PLoS Genetics, 2016
Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this.
N. Wiechens, Vijender Singh, Triantaffyllos Gkikopoulos, Pietá Schofield, S. Rocha, T. Owen-Hughes   +5 more
semanticscholar   +1 more source

DNA Nanomechanics in the Nucleosome [PDF]

Structure, 2009
The sequence-dependent mechanics of DNA-histone binding are essential for nucleosome positioning and mobility. Here we reanalyze nucleosome crystal structures in terms of the well-characterized base-pair scale DNA elasticity, extracting the forces and torques acting on all bound DNA base pairs.
Ralf Everaers, Nils B. Becker
openaire   +3 more sources

IncRNA‐ZFAS1, an Emerging Gate‐Keeper in DNA Damage‐Dependent Transcriptional Regulation

Advanced Science, EarlyView.
LncZFAS1 plays a crucial role during DNA damage response in mammalian cells. Loss of lncZFAS1 results in deficient DNA lesion removal and reduced cell viability. Mechanistically, lncZFAS1 modulates RNAPII phosphorylation and transcription and thereby promotes both GG‐NER and TC‐NER upon UV damage.
Jiena Liu, Qing Lu, Zixuan Fan, Jiahui Lin, Nan He, Xin Zhang, Zhaoya Han, Tingting Zhu, Zhenzhen Wu, Yingying Xu, Yuming Wang   +10 more
wiley   +1 more source