Results 11 to 20 of about 12,120 (223)

Cell sources for nucleus pulposus regeneration

European Spine Journal, 2013
There is increasing interest in the development of cell therapy as a possible approach for the treatment of degenerative disc disease. To regenerate nucleus pulposus tissue, the cells must produce an appropriate proteoglycan-rich matrix, as this is essential for the functioning of the intervertebral disc. The natural environment within the disc is very
Kregar Velikonja, N, Urban, J, Fröhlich, M, Neidlinger-Wilke, C, Kletsas, D, Potocar, U, Turner, S, Roberts, S   +7 more
openaire   +4 more sources

Harmonization and standardization of nucleus pulposus cell extraction and culture methods [PDF]

JOR SPINE, 2023
AbstractBackgroundIn vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes the much‐needed progress in the field.
Basatvat, Shaghayegh, Bach, Frances C., Barcellona, Marcos N., Binch, Abbie L., Buckley, Conor T., Bueno, Brian, Chahine, Nadeen O., Chee, Ana, Creemers, Laura B., Dudli, Stefan, Fearing, Bailey, Ferguson, Stephen J., Gansau, Jennifer, Gantenbein, Benjamin, Gawri, Rahul, Glaeser, Juliane D., Grad, Sibylle, Guerrero, Julien, Haglund, Lisbet, Hernandez, Paula A., Hoyland, Judith A., Huang, Charles, Iatridis, James C., Illien‐Junger, Svenja, Jing, Liufang, Kraus, Petra, Laagland, Lisanne T., Lang, Gernot, Leung, Victor, Li, Zhen, Lufkin, Thomas, van Maanen, Josette C., McDonnell, Emily E., Panebianco, Chris J., Presciutti, Steven M., Rao, Sanjna, Richardson, Stephen M., Romereim, Sarah, Schmitz, Tara C., Schol, Jordy, Setton, Lori, Sheyn, Dmitriy, Snuggs, Joseph W., Sun, Y., Tan, Xiaohong, Tryfonidou, Marianna A., Vo, Nam, Wang, Dong, Williams, Brandon, Williams, Rebecca, Yoon, S. Tim, Le Maitre, Christine L.   +51 more
openaire   +7 more sources

Scutellarin reduces apoptosis of nucleus pulposus cells in intervertebral disc degeneration of rat models [PDF]

Jichu yixue yu linchuang
Objective To investigate the effects of scutellarin (SCU) on apoptosis of nucleus pulposus cells and the Janus kinas 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in intervertebral disc degeneration (IVDD) model rats.
LI Na, ZHANG Peng, ZHANG Nan, LIN Wei, LIU Defeng, ZHENG Jihui
doaj   +2 more sources

MiR-27a regulates apoptosis in nucleus pulposus cells by targeting PI3K. [PDF]

PLoS ONE, 2013
The precise role of apoptosis in the pathogenesis of intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that the expression of miR-27a was increased.
Gang Liu, Peng Cao, Huajiang Chen, Wen Yuan, Jianxi Wang, Xianye Tang   +5 more
doaj   +2 more sources

Hemeoxygenase-1 Suppresses IL-1β-Induced Apoptosis Through the NF-κB Pathway in Human Degenerative Nucleus Pulposus Cells

Cellular Physiology and Biochemistry, 2018
Background/Aims: Nucleus pulposus cell (NPC) apoptosis is the main factor in intervertebral disc degeneration (IDD); thus, inhibiting the excessive apoptosis of nucleus pulposus cells may be a potential way to alleviate IDD. The effect of Hemeoxygenase-1
Chaoying Zhu, Wei Jiang, Qiang Cheng, Zhenming Hu, Jie Hao   +4 more
doaj   +3 more sources

TGF-β Induces Up-Regulation of Chondroitin Sulfate Synthase 1 (CHSY1) in Nucleus Pulposus Cells Through MAPK Signaling [PDF]

Cellular Physiology and Biochemistry, 2015
Background/Aims: Chondroitin sulfate synthase 1 (CHSY1) is a glycosyltransferases involved in the biosynthesis of chondroitin and dermatan sulfate glycosaminoglycan (GAG).
Bo Hu, Changgui Shi, Ye Tian, Ying Zhang, Chen Xu, Huajiang Chen, Peng Cao, Wen Yuan   +7 more
doaj   +2 more sources

Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-α-induced senescence

Stem Cell Research & Therapy, 2018
Background Cell therapy for the treatment of intervertebral disc degeneration (IDD) faces serious barriers since tissue-specific adult cells such as nucleus pulposus cells (NPCs) have limited proliferative ability and poor regenerative potential; in ...
Xiao-Chuan Li, Mao-Sheng Wang, Wei Liu, Cheng-Fan Zhong, Gui-Bin Deng, Shao-Jian Luo, Chun-Ming Huang   +6 more
doaj   +2 more sources

Cell sources proposed for nucleus pulposus regeneration [PDF]

JOR SPINE, 2021
AbstractLower back pain (LBP) occurs in 80% of adults in their lifetime; resulting in LBP being one of the biggest causes of disability worldwide. Chronic LBP has been linked to the degeneration of the intervertebral disc (IVD). The current treatments for chronic back pain only provide alleviation of symptoms through pain relief, tissue removal, or ...
Williams, Rebecca J., Tryfonidou, Marianna A., Snuggs, Joseph Wiliam, Le Maitre, Christine Lyn   +3 more
openaire   +7 more sources

SETD1A Regulates Glycolysis and Senescence of Nucleus Pulposus Cells via H3K4me3-HELZ2/PPARα-HIF1α Axis to Drive Intervertebral Disc Degeneration. [PDF]

Adv Sci (Weinh)
SETD1A is a key epigenetic regulator in NPCs during IDD. In normal NPCs, it sustains H3K4me3–HELZ2/PPARα–HIF1α signaling to maintain glycolytic energy metabolism and proliferation. In degenerated NPCs, reduced SETD1A disrupts this axis, impairing glycolysis and accelerating senescence, highlighting a promising therapeutic target for IDD.
Fu J, Leng X, Long J, Liao Z, An X, Ai X, Long D, Li C, Huang B, Zhou Y, Dong S, Feng C.   +11 more
europepmc   +2 more sources

Alterations of bovine nucleus pulposus cells with aging

Aging Cell, 2023
AbstractAging is one of the major etiological factors driving intervertebral disc (IVD) degeneration, the main cause of low back pain. The nucleus pulposus (NP) includes a heterogeneous cell population, which is still poorly characterized. Here, we aimed to uncover main alterations in NP cells with aging. For that, bovine coccygeal discs from young (12 
Maria Molinos, Morena F. Fiordalisi, Joana Caldeira, Catarina R. Almeida, Mário A. Barbosa, Raquel M. Gonçalves   +5 more
openaire   +3 more sources