Results 131 to 140 of about 14,685,807 (300)

Using RNA-seq to Assess Off-Target Effects of Antisense Oligonucleotides in Human Cell Lines

, 2020
International audienceBackground The field of antisense oligonucleotide therapeutics is rapidly growing and in addition to small molecules and therapeutic antibodies, oligonucleotide-based gene expression modifiers have been developed as fully accepted ...
Shen, Yimin, Jaschinski, Frank, Klar, Richard, Tost, Jörg, Michel, Sven, Schirduan, Ksenija, Richard Klar, Jörg Tost, Ksenija Schirduan, Frank Jaschinski, Yimin Shen, Sven Michel   +11 more
core   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

A novel base editor SpRY-ABE8e^F148A mediates efficient A-to-G base editing with a reduced off-target effect. [PDF]

Mol Ther Nucleic Acids, 2023
Li G, Cheng Y, Li Y, Ma H, Pu Z, Li S, Zhao Y, Huang X, Yao Y.   +8 more
europepmc   +1 more source

Haploidentical Hematopoietic Cell Transplantation for Infants With X‐Linked Severe Combined Immunodeficiency Using Post‐Transplant Cyclophosphamide

Pediatric Blood &Cancer, EarlyView.
Shuhei Takahashi, Akito Kawachi, Dan Tomomasa, Eri Watanabe, Yuji Fujita, Yuya Sato, Masahiro Sekiguchi, Kentaro Watanabe, Moe Hidaka, Akihiro Hoshino, Takahiro Kamiya, Masatoshi Takagi, Hirokazu Kanegane, Motohiro Kato   +13 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Human 8-cell embryos enable efficient induction of disease-preventive mutations without off-target effect by cytosine base editor. [PDF]

Protein Cell, 2023
Wei Y, Zhang M, Hu J, Zhou Y, Xue M, Yin J, Liu Y, Feng H, Zhou L, Li Z, Wang D, Zhang Z, Zhou Y, Liu H, Yao N, Zuo E, Hu J, Du Y, Li W, Xu C, Yang H, Yang H.   +21 more
europepmc   +1 more source

Off-target effects of oligonucleotides and approaches of preclinical assessments

SLAS Discovery
Oligonucleotide-based therapies, such as antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), represent a class of therapeutic agents that specifically target gene transcription or translation mechanisms through sequence specificity. These pharmaceuticals exhibit significant promise in the treatment of genetic disorders, including spinal
Haiwen Ruan, Dehu Dou, Jing Lu, Xia Xiao, Xinjiang Gong, Xuefeng Zhang   +5 more
openaire   +2 more sources