Results 141 to 150 of about 13,860,386 (295)
Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo. [PDF]
Sci Rep, 2022 Piao X +5 more
europepmc +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Mechanistic mathematical modeling of abscopal effect reveals mechanisms of off-target tumor response
Frontiers in ImmunologyIntroductionLocal radiotherapy rarely triggers regression of distant, non-irradiated tumors (the “abscopal” effect), but this outcome is unpredictable because it depends on interacting processes, such as antigen release, antigen presentation, T-cell ...
Andreas G. Hadjigeorgiou +5 more
doaj +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Triazolinedione protein modification: from an overlooked off-target effect to a tryptophan-based bioconjugation strategy. [PDF]
Chem Sci, 2022 Decoene KW +7 more
europepmc +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
, 2019 Additional file 2: Figure S2. No off-target effects were found in all the offspring of mice that did not carry off-target locus. (A) PCR identifications of KI offspring of mice not carrying #1 off-target locus were validated by gel electrophoresis.
Junchao Dong (7582535) +4 more
core +1 more source
Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. [PDF]
Mol Ther, 2022 Zhou J +10 more
europepmc +1 more source
FEBS Letters, EarlyView.Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani +3 more
wiley +1 more source