Results 151 to 160 of about 14,685,807 (300)
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Potential off-target sites in mouse genome.
, 2015 Genomic sequences of off-target sites with PAM sequences, their locations, annotations, and the number of base pair differences are noted. Bold letters indicate mismatched nucleotides.Potential off-target sites in mouse genome.
Kentaro Tsukamoto (770681) +3 more
core +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Triazolinedione protein modification: from an overlooked off-target effect to a tryptophan-based bioconjugation strategy. [PDF]
Chem Sci, 2022 Decoene KW +7 more
europepmc +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. [PDF]
Mol Ther, 2022 Zhou J +10 more
europepmc +1 more source
Specific cut-off values for each target.
, 2015 *Anti-PVL_2 Antibody detects exclusive epitopes of lukF-P83. Positive signals for two of the anti-PVL antibodies, 1, 2 or 3, indicate for the target lukF-PV.Specific cut-off values for each target.
Bettina Stieber (524740) +4 more
core +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
An EZH2 blocker sensitizes histone mutated diffuse midline glioma to cholesterol metabolism inhibitors through an off-target effect. [PDF]
Neurooncol Adv, 2022 Rahal F +13 more
europepmc +1 more source