Off-target effects of CRISPRa on interleukin-6 expression
PLOS ONE, 2019 Inactive fusion variants of the CRISPR-Cas9 system are increasingly being used as standard methodology to study transcription regulation. Their ability to readily manipulate the native genomic loci is particularly advantageous. In this work, we serendipitously uncover the key cytokine IL6 as an off-target of the activating derivative of CRISPR (CRISPRa)
Sébastien Soubeyrand +3 more
openaire +5 more sources
Whole-genome thermodynamic analysis reduces siRNA off-target effects. [PDF]
PLoS ONE, 2013 Small interfering RNAs (siRNAs) are important tools for knocking down targeted genes, and have been widely applied to biological and biomedical research.
Xi Chen, Peng Liu, Hui-Hsien Chou
doaj +2 more sources
The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects. [PDF]
Genes (Basel), 2022 In RNA interference (RNAi), small interfering RNA (siRNA) suppresses the expression of its target mRNA with a perfect complementary sequence. In addition, siRNA also suppresses the expression of unintended mRNAs with partially complementary sequences ...
Kobayashi Y, Tian S, Ui-Tei K.
europepmc +2 more sources
siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect [PDF]
BMC Bioinformatics, 2009 Background RNA interference (RNAi), mediated by 21-nucleotide (nt)-length small interfering RNAs (siRNAs), is a powerful tool not only for studying gene function but also for therapeutic applications.
Morishita Shinichi +3 more
doaj +2 more sources
The siRNA Non-seed Region and Its Target Sequences Are Auxiliary Determinants of Off-Target Effects.
PLoS Computational Biology, 2015 RNA interference (RNAi) is a powerful tool for post-transcriptional gene silencing. However, the siRNA guide strand may bind unintended off-target transcripts via partial sequence complementarity by a mechanism closely mirroring micro RNA (miRNA ...
Piotr J Kamola +4 more
doaj +2 more sources
Cross-border acquisitions into the UK: an analysis of target company returns [PDF]
, 2000 In this paper we investigate whether there is evidence of a target company cross-border effect by comparing the premium for those companies acquired by other UK companies with the premium for those companies acquired by non-UK firms.
Danbolt, J.
core +8 more sources
Frontiers in Microbiology, 2021 Viral DNA integrated in host cells is a major barrier to completely curing HIV-1. However, genome editing using the recently developed technique of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has the potential to eradicate HIV-
Yufan Xu +8 more
doaj +1 more source
Reduction in PCSK9 levels induced by anacetrapib: an off-target effect?1 [PDF]
Journal of Lipid Research, 2015 Philip J. Barter +2 more
doaj +2 more sources
Off-target profiling of tofacitinib and baricitinib by machine learning: a focus on thrombosis and viral infection. [PDF]
, 2021 Objectives: There are no clear on-target mechanisms that explain the increased risk for thrombosis and viral infection or reactivation associated with Janus kinase (JAK) inhibitors.
Maria Luisa , Faquetti +4 more
core +2 more sources
CRISPR-Cas9 System for Plant Genome Editing: Current Approaches and Emerging Developments
Agronomy, 2020 Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. This editing technology has been in the limelight due to its simplicity and versatility compared to other previously known ...
Jake Adolf V. Montecillo +2 more
doaj +1 more source