Results 41 to 50 of about 14,685,807 (300)
Development of therapeutic-grade small interfering RNAs by chemical engineering
Frontiers in Genetics, 2012 Recent successes in clinical trials have provided important proof of concept that small interfering RNAs indeed constitute a new promising class of therapeutics.
Jesper Bertram Bramsen, Jørgen eKjems
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Off‐target effects of engineered nucleases [PDF]
The FEBS Journal, 2016 Recent advances in gene editing with engineered nucleases have transformed our ability to manipulate the genome from diverse organisms for applications ranging from biomedical research to disease treatment. A major complication with these engineered nucleases is the binding of the nuclease to unintended genomic sites that share sequence homology with ...
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Molecular Therapy: Nucleic Acids, 2023 Small interfering RNA (siRNA) and short hairpin RNA (shRNA) are widely used as RNA interference (RNAi) reagents. Recently, truncated shRNAs that trigger RNAi in a Dicer-independent manner have been developed.
Atsushi Shibata +6 more
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Off-target effects of siRNA specific for GFP [PDF]
BMC Molecular Biology, 2008 Gene knock down by RNAi is a highly effective approach to silence gene expression in experimental as well as therapeutic settings. However, this widely used methodology entails serious pitfalls, especially concerning specificity of the RNAi molecules.We tested the most widely used control siRNA directed against GFP for off-target effects and found that
Tschuch, Cordula +8 more
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Off-target prediction to p.Trp402* sgRNA by predictors and filter criterias.
, 2022 Graphic illustration of the off-target prediction to p.Trp402* sgRNA before and after the filtering steps. The image shows the number of sequences returned for each of the five predictors and the number of sequences after the filtering step following the
Ursula Matte (328018) +2 more
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Off-Target Effects of Mirabegron on Muscarinic Receptors
Future PharmacologyOlder adults with multiple diseases are likely to be prescribed multiple medications including anticholinergic agents, which are frequently prescribed to manage conditions such as overactive bladder and chronic obstructive pulmonary disease and Parkinson’
Shizuo Yamada +3 more
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, 2018 (A) The target site (T) and ten predicted off-target sites (OT) for sgRNA91/Cas9. OT1~OT10 represents 10 potential off-target sites, and T represents target site.
Hongming Yuan (2200930) +20 more
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Translation of CRISPR Genome Surgery to the Bedside for Retinal Diseases
Frontiers in Cell and Developmental Biology, 2018 In recent years, there has been accelerated growth of clustered regularly interspaced short palindromic repeats (CRISPR) genome surgery techniques. Genome surgery holds promise for diseases for which a cure currently does not exist.
Christine L. Xu +15 more
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On-target and Off-target-based Toxicologic Effects [PDF]
Toxicologic Pathology, 2012 Adverse toxicologic effects are categorized as chemical-based, on-target, or off-target effects. Chemical-based toxicity is defined as toxicity that is related to the physicochemical characteristics of a compound and its effects on cellular organelles, membranes, and/or metabolic pathways.
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, 2014 Supplemental file with off-target sequences obtained using primers designed to specifically amplify bacterial endosymbionts.
Robert W. Bryson (3259062)
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