Results 31 to 40 of about 13,860,386 (295)
siRNA design software for a target gene-specific RNA interference
Frontiers in Genetics, 2012 RNA interference (RNAi) is the mechanism through which small interfering RNA (siRNA) induces sequence-specific posttranscriptional gene silencing. RNAi is commonly recognized as a powerful tool not only for functional genomics but also for therapeutic ...
Yuki eNaito +3 more
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Off-target effects of torcetrapib [PDF]
Nature Reviews Cardiology, 2010 The adverse off-target effects of the cholesteryl ester transfer protein (CETP) inhibitor torcetrapib may be mediated by L-type calcium channels, shows a study published in the Journal of Hypertension.
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Off- and on-target effects of genome editing in mouse embryos
The Journal of Reproduction and Development, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE +2 more
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, 2023 Off-targets of PPR56, PPR56|S4TN>TD and PPR56|S10TD>TN in the E.coli transcriptome summarized with Weblogo [89]. Consensus profiles were created from the sequences of 119, 382 and 15 C-to-U RNA editing off-targets, weighted with their respective editing ...
Jingchan Xie (16859288) +5 more
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Oncogenesis: An “Off-Target” Effect of Radiofrequency Ablation [PDF]
Radiology, 2015 To compare hepatocellular carcinoma (HCC) development after radiofrequency (RF) ablation, partial surgical hepatectomy, and a sham operation and to inhibit HCC recurrence after RF ablation in a mouse model of spontaneously forming HCC in the setting of chronic inflammation (ie, the MDR2 knockout model).Animal experiments were performed according to an ...
Nir, Rozenblum +7 more
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BMC Medical Informatics and Decision Making, 2020 Background It is of utmost importance to investigate novel therapies for cancer, as it is a major cause of death. In recent years, immunotherapies, especially those against immune checkpoints, have been developed and brought significant improvement in ...
Yuyu Zheng +4 more
doaj +1 more source
Thermodynamic control of small RNA-mediated gene silencing
Frontiers in Genetics, 2012 Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are crucial regulators of posttranscriptional gene silencing, which is referred to as RNA interference (RNAi) or RNA silencing.
Kumiko eUi-Tei +3 more
doaj +1 more source
Frontiers in Molecular Neuroscience, 2021 Variants of the SH3 and multiple ankyrin repeat domains 3 (SHANK3), which encodes postsynaptic scaffolds, are associated with brain disorders. The targeted alleles in a few Shank3 knock-out (KO) lines contain a neomycin resistance (Neo) cassette, which ...
Chunmei Jin +26 more
doaj +1 more source
Off‐target effects of engineered nucleases [PDF]
The FEBS Journal, 2016 Recent advances in gene editing with engineered nucleases have transformed our ability to manipulate the genome from diverse organisms for applications ranging from biomedical research to disease treatment. A major complication with these engineered nucleases is the binding of the nuclease to unintended genomic sites that share sequence homology with ...
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Off-target effects of siRNA specific for GFP [PDF]
BMC Molecular Biology, 2008 Gene knock down by RNAi is a highly effective approach to silence gene expression in experimental as well as therapeutic settings. However, this widely used methodology entails serious pitfalls, especially concerning specificity of the RNAi molecules.We tested the most widely used control siRNA directed against GFP for off-target effects and found that
Tschuch, Cordula +8 more
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