Results 31 to 40 of about 14,685,807 (300)

siRNA design software for a target gene-specific RNA interference

Frontiers in Genetics, 2012
RNA interference (RNAi) is the mechanism through which small interfering RNA (siRNA) induces sequence-specific posttranscriptional gene silencing. RNAi is commonly recognized as a powerful tool not only for functional genomics but also for therapeutic ...
Yuki eNaito, Yuki eNaito, Kumiko eUi-Tei, Kumiko eUi-Tei   +3 more
doaj   +1 more source

Off- and on-target effects of genome editing in mouse embryos

The Journal of Reproduction and Development, 2018
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE, Kenichi NAKASHIMA, Atsushi YOSHIKI   +2 more
doaj   +1 more source

Hybrid phenotype mining method for investigating off-target protein and underlying side effects of anti-tumor immunotherapy

BMC Medical Informatics and Decision Making, 2020
Background It is of utmost importance to investigate novel therapies for cancer, as it is a major cause of death. In recent years, immunotherapies, especially those against immune checkpoints, have been developed and brought significant improvement in ...
Yuyu Zheng, Xiangyu Meng, Pierre Zweigenbaum, Lingling Chen, Jingbo Xia   +4 more
doaj   +1 more source

Baricitinib and tofacitinib off-target profile, with a focus on Alzheimer’s disease [PDF]

, 2023
Introduction: Janus Kinase (JAK) inhibitors were recently identified as promising drug candidates for repurposing in Alzheimer’s disease (AD) due to their capacity to suppress inflammation via modulation of JAK/STAT signalling pathways.
Maria Luisa , Faquetti, Laura, Slappendel, Francesca , Grisoni, Shana, Sturla, Andrea, Burden, Gisbert , Schneider, Hélène, Bigonne, Petra , Schneider, Georg, Aichinger   +8 more
core   +2 more sources

Structural basis for Cas9 off-target activity [PDF]

, 2022
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is determined by complementarity to a 20-nucleotide segment in its guide RNA.
Allain, Frédéric H-T; https://orcid.org/, Donohoue, Paul D, Jinek, Martin; https://orcid.org/, Pacesa, Martin; https://orcid.org/, Cléry, Antoine; https://orcid.org/, Palermo, Giulia; https://orcid.org/, Arantes, Pablo R; https://orcid.org/, Cameron, Peter, Saha, Aakash; https://orcid.org/, Irby, Matthew J, Lin, Chun-Han, Bargsten, Katja   +11 more
core   +1 more source

The Neomycin Resistance Cassette in the Targeted Allele of Shank3B Knock-Out Mice Has Potential Off-Target Effects to Produce an Unusual Shank3 Isoform

Frontiers in Molecular Neuroscience, 2021
Variants of the SH3 and multiple ankyrin repeat domains 3 (SHANK3), which encodes postsynaptic scaffolds, are associated with brain disorders. The targeted alleles in a few Shank3 knock-out (KO) lines contain a neomycin resistance (Neo) cassette, which ...
Chunmei Jin, Chunmei Jin, Hyojin Kang, Taesun Yoo, Jae Ryun Ryu, Ye-Eun Yoo, Ruiying Ma, Ruiying Ma, Yinhua Zhang, Yinhua Zhang, Hyae Rim Kang, Hyae Rim Kang, Yoonhee Kim, Hyunyoung Seong, Geul Bang, Geul Bang, Sangwoo Park, Seok-Kyu Kwon, Woong Sun, Woong Sun, Hyunkyung Kim, Hyunkyung Kim, Jin Young Kim, Eunjoon Kim, Eunjoon Kim, Kihoon Han, Kihoon Han   +26 more
doaj   +1 more source

Oncogenesis: An “Off-Target” Effect of Radiofrequency Ablation [PDF]

Radiology, 2015
To compare hepatocellular carcinoma (HCC) development after radiofrequency (RF) ablation, partial surgical hepatectomy, and a sham operation and to inhibit HCC recurrence after RF ablation in a mouse model of spontaneously forming HCC in the setting of chronic inflammation (ie, the MDR2 knockout model).Animal experiments were performed according to an ...
Nir, Rozenblum, Evelyne, Zeira, Viviana, Scaiewicz, Baruch, Bulvik, Svetlana, Gourevitch, Hagit, Yotvat, Eithan, Galun, S Nahum, Goldberg   +7 more
openaire   +2 more sources

Thermodynamic control of small RNA-mediated gene silencing

Frontiers in Genetics, 2012
Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are crucial regulators of posttranscriptional gene silencing, which is referred to as RNA interference (RNAi) or RNA silencing.
Kumiko eUi-Tei, Kenji eNishi, Tomoko eTakahashi, Tatsuya eNagasawa   +3 more
doaj   +1 more source

Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities

Molecular Therapy: Nucleic Acids, 2022
Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of ...
Zhenwu Zhang, Wanyu Tao, Shisheng Huang, Wenjun Sun, Yue Wang, Wen Jiang, Xingxu Huang, Chao-Po Lin   +7 more
doaj   +1 more source

Principal component analyses of progressively filtered off-target SNP datasets.

, 2021
Clustering of the 96 arctic fox samples based on on-target SNPs throughout filtering steps. a) PCA of all off-target SNPs after filtering for MAF, missing-data, and biallelic SNPs (n = 6432) and b) PCA of all identified off-target SNPs after analysis ...
Tristan M. Baecklund (10573715), Michael E. Donaldson (4431115), Karsten Hueffer (606200), Christopher J. Kyle (8136867)   +3 more
core   +1 more source