Results 21 to 30 of about 13,860,386 (295)
Journal of Integrative Agriculture, 2022 A recent breakthrough in agricultural biotechnology is the introduction of RNAi-mediated strategies in pest control. However, the off-target effects of RNAi pest control are still not fully understood.
Wei-hua MA +10 more
doaj +1 more source
Off-Target Effects of MEK Inhibitors [PDF]
Biochemistry, 2013 The mitogen-activated protein kinases (MAPKs) ERK1/2 regulate numerous cellular processes, including gene transcription, proliferation, and differentiation. The only known substrates of the MAP2Ks MEK1/2 are ERK1/2; thus, MEK inhibitors PD98059, U0126, and PD0325901 have been important tools in determining the functions of ERK1/2.
Eric M, Wauson +4 more
openaire +2 more sources
Molecular Therapy: Nucleic Acids, 2023 Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3′-overhanging ends, limiting its applications.
Yue Wang +6 more
doaj +1 more source
Baricitinib and tofacitinib off-target profile, with a focus on Alzheimer’s disease [PDF]
, 2023 Introduction: Janus Kinase (JAK) inhibitors were recently identified as promising drug candidates for repurposing in Alzheimer’s disease (AD) due to their capacity to suppress inflammation via modulation of JAK/STAT signalling pathways.
Maria Luisa , Faquetti +8 more
core +2 more sources
Quantifying CRISPR off-target effects [PDF]
Emerging Topics in Life Sciences, 2019 Abstract Recent advances in the era of genetic engineering have significantly improved our ability to make precise changes in the genomes of human cells. Throughout the years, clinical trials based on gene therapies have led to the cure of diseases such as X-linked severe combined immunodeficiency (SCID-X1), adenosine deaminase ...
openaire +4 more sources
Molecular Therapy: Nucleic Acids, 2022 The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park +8 more
doaj +1 more source
Off-target sequences inside repetitive elements.
, 2022 Off-target sequences inside repetitive elements.
Ursula Matte (328018) +2 more
core +1 more source
Prediction of off-target effects of the CRISPR/Cas9 system for design of sgRNA [PDF]
E3S Web of Conferences, 2020 CRISPR/Cas9 genome editing technology is the frontier of life science research. They have been used to cure human genetic diseases, achieve cell personalized treatment, develop new drugs, and improve the genetic characteristics of crops and other fields.
Guo Calvin, Zhen David
doaj +1 more source
Frontiers in Oncology, 2019 Background: Localized radiation therapy (RT) is known to infrequently cause off-target or “abscopal” effects at distant metastatic lesions. The mechanism through which abscopal effects occur remains unknown, but is thought to be caused by a humoral ...
Danielle Golub +4 more
doaj +1 more source
Strategies for High-Efficiency Mutation Using the CRISPR/Cas System
Frontiers in Cell and Developmental Biology, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems have revolutionized traditional gene-editing tools and are a significant tool for ameliorating gene defects.
Shuying Feng +10 more
doaj +1 more source