Results 21 to 30 of about 14,685,807 (300)
Journal of Integrative Agriculture, 2022 A recent breakthrough in agricultural biotechnology is the introduction of RNAi-mediated strategies in pest control. However, the off-target effects of RNAi pest control are still not fully understood.
Wei-hua MA +10 more
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Off-Target Effects of MEK Inhibitors [PDF]
Biochemistry, 2013 The mitogen-activated protein kinases (MAPKs) ERK1/2 regulate numerous cellular processes, including gene transcription, proliferation, and differentiation. The only known substrates of the MAP2Ks MEK1/2 are ERK1/2; thus, MEK inhibitors PD98059, U0126, and PD0325901 have been important tools in determining the functions of ERK1/2.
Eric M, Wauson +4 more
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Molecular Therapy: Nucleic Acids, 2023 Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3′-overhanging ends, limiting its applications.
Yue Wang +6 more
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Quantifying CRISPR off-target effects [PDF]
Emerging Topics in Life Sciences, 2019 Abstract Recent advances in the era of genetic engineering have significantly improved our ability to make precise changes in the genomes of human cells. Throughout the years, clinical trials based on gene therapies have led to the cure of diseases such as X-linked severe combined immunodeficiency (SCID-X1), adenosine deaminase ...
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Off-target sequences inside repetitive elements.
, 2022 Off-target sequences inside repetitive elements.
Ursula Matte (328018) +2 more
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Molecular Therapy: Nucleic Acids, 2022 The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park +8 more
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Effect of polymorphic sites in off-target sequences in mismatches and indels amount.
, 2022 Effect of polymorphic sites in off-target sequences in mismatches and indels amount.
Ursula Matte (328018) +2 more
core +1 more source
Frontiers in Oncology, 2019 Background: Localized radiation therapy (RT) is known to infrequently cause off-target or “abscopal” effects at distant metastatic lesions. The mechanism through which abscopal effects occur remains unknown, but is thought to be caused by a humoral ...
Danielle Golub +4 more
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Strategies for High-Efficiency Mutation Using the CRISPR/Cas System
Frontiers in Cell and Developmental Biology, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems have revolutionized traditional gene-editing tools and are a significant tool for ameliorating gene defects.
Shuying Feng +10 more
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Off-target effects of torcetrapib [PDF]
Nature Reviews Cardiology, 2010 The adverse off-target effects of the cholesteryl ester transfer protein (CETP) inhibitor torcetrapib may be mediated by L-type calcium channels, shows a study published in the Journal of Hypertension.
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