Results 11 to 20 of about 11,991 (190)

Use of a Dihydroxyacetone Derivative as Protecting Reagent to Phosphorylate Oligonucleotides. [PDF]

Curr Protoc
Abstract We present here a new reagent enabling the supported synthesis of oligodeoxynucleotides (ODNs) and oligoribonucleotides (ORNs) containing a phosphate group at the 5′‐terminal position after complete deprotection. This reagent, derived from a dihydroxyacetone core, contains a dimethoxytrityl (DMTr) group. The procedure for final deprotection is
Lartia R.
europepmc   +2 more sources

Glutathione Mediates Control of Dual Differential Bio‐orthogonal Labelling of Biomolecules

Angewandte Chemie, Volume 135, Issue 50, December 11, 2023., 2023
The chemical modification at precise sites within biomolecules is essential to assist in understanding their function. The incorporation of bio‐orthogonal reactive groups provides a strategy for selective tagging, however cross‐reactivity is problematic when dual modification is required.
Frederik Peschke, Andrea Taladriz‐Sender, Matthew J. Andrews, Allan J. B. Watson, Glenn A. Burley   +4 more
wiley   +2 more sources

Microwave-assisted rapid deprotection of oligodeoxyribonucleotides [PDF]

Nucleic Acids Research, 1997
A novel method for the deprotection of oligodeoxyribonucleotides under microwave irradiation has been developed. The oligodeoxynucleotides having base labile, phenoxyacetyl (pac), protection for exocyclic amino functions were fully deprotected in 0. 2 M sodium hydroxide (methanol:water : : 1:1, v/v) = A and 1 M sodium hydroxide (methanol:water : : 1:1,
P, Kumar, K C, Gupta
openaire   +2 more sources

Photoinduced Reactions of Styrylpyridine Derivatives for the In Situ Formation of Selective Ligands for Apyrimidinic DNA

ChemPhotoChem, Volume 7, Issue 12, December 2023., 2023
The photoreaction of a styrylpyridine derivative leads to a DNA‐binding benzoquinolizinium derivative with selectivity towards abasic site‐containing DNA (AP‐DNA), as caused by the sterically demanding substituents. Notably, the DNA binding is accomplished by photoinduced in situ formation of the ligand in the presence of AP‐DNA.
Julika Schlosser, Nils Stötzel, Yuri Fedorov, Heiko Ihmels   +3 more
wiley   +1 more source

Convenient synthesis of arabinonucleoside containing oligodeoxyribonucleotides [PDF]

Nucleic Acids Symposium Series, 2000
C2 substituted arabinofuranosyluracil derivatives were synthesized and its incorporations into DNA were easily carried out by using post-synthetic modification.
H, Ozaki, K, Nakajima, C, Izumi, H, Sawai   +3 more
openaire   +2 more sources

Gene therapy in cancer

The Journal of Gene Medicine, Volume 25, Issue 11, November 2023., 2023
Cancer is a heterogeneous and complex disease in which some cells in the body proliferate uncontrollably and invade other tissues and individual differences are quite effective. Treatment using advanced therapy medicinal products, one of which is gene therapy, is gaining importance by the develeopment of biotechnology. Gene therapy strategies in cancer
Büşra Cesur‐Ergün, Devrim Demir‐Dora   +1 more
wiley   +1 more source

Application of the weighted histogram method for calculating the thermodynamic parameters of the formation of oligodeoxyribonucleotide duplexes

Вавиловский журнал генетики и селекции, 2023
To date, many derivatives and analogs of nucleic acids (NAs) have been developed. Some of them have found uses in scientific research and biomedical applications. Their effective use is based on the data about their properties. Some of the most important
I. I. Yushin, V. M. Golyshev, D. V. Pyshnyi, A. A. Lomzov   +3 more
doaj   +1 more source

Determination of Methylation Site of DNA-Methyltransferase NlaX by a Hybrid Method

BioTechniques, 2002
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M·NlaX) from Neisseria lactamica was established to be
E.A. Kubareva, J. Walter, A.S. Karyagina, O.V. Vorob'eva, P.C.K. Lau, T. Trautner   +5 more
doaj   +1 more source

An experimental study of the effects of SNPs in the TATA boxes of the GRIN1, ASCL3 and NOS1 genes on interactions with the TATA-binding protein

Вавиловский журнал генетики и селекции, 2022
The GRIN1, ASCL3, and NOS1 genes are associated with various phenotypes of neuropsychiatric disorders. For instance, these genes contribute to the development of schizophrenia, Alzheimer’s and Parkinson’s diseases, and epilepsy.
E. B. Sharypova, I. A. Drachkova, I. V. Chadaeva, M. P. Ponomarenko, L. K. Savinkova   +4 more
doaj   +1 more source

Frontiers and Approaches to Chemical Synthesis of Oligodeoxyribonucleotides

Molecules, 2013
The advantages and disadvantages of existing approaches to the synthesis of oligodeoxyribonucleotides (ODN) are discussed focusing on large-scale methods. The liquid phase and solid supported synthesis and the synthesis on soluble polymers are discussed.
Tatyana Abramova
doaj   +1 more source