Results 21 to 30 of about 11,432 (197)

The Potential of Telomeric G-Quadruplexes Containing Modified Oligoguanosine Overhangs in Activation of Bacterial Phagocytosis and Leukotriene Synthesis in Human Neutrophils

Biomolecules, 2020
Human neutrophils are the first line of defense against bacterial and viral infections. They eliminate pathogens through phagocytosis, which activate the 5-lipoxygenase (5-LOX) pathway resulting in synthesis of leukotrienes.
Ekaterina A. Golenkina, Galina M. Viryasova, Nina G. Dolinnaya, Valeria A. Bannikova, Tatjana V. Gaponova, Yulia M. Romanova, Galina F. Sud’ina   +6 more
doaj   +1 more source

Anomalous hairpin formation in an oligodeoxyribonucleotide [PDF]

Nucleic Acids Research, 1985
An accurate method for deriving molar absorptivity-temperature profiles applied to a set of single-stranded oligodeoxyribonucleotides shows that the undecamer CGAGTTTGACGp exists in a hairpin conformation involving Watson-Crick base pairing between the two terminal CG dinucleotides.
J G, Nadeau, P T, Gilham
openaire   +2 more sources

Position-Dependent Stabilization of DNA/RNA Duplexes by Site-Specific Incorporation of LNA Nucleosides. [PDF]

J Nucleic Acids
Owing to their enhanced functional properties, 2 ′,4 ′‐bridged nucleic acids/locked nucleic acids (2 ′,4 ′‐BNAs/LNAs) are considered promising candidates for antisense oligonucleotide therapeutics. The spatial arrangement of LNA residues within the oligonucleotide sequence is a key determinant of binding affinity to the target RNA.
Tomita-Sudo E, Akita T, Sakimoto N, Tahara-Takamine S, Sano R, Iwai S, Kawakami J.   +6 more
europepmc   +2 more sources

Synthesis and radioiodination of a stannyl oligodeoxyribonucleotide [PDF]

Nucleic Acids Research, 1997
Synthesis and radioiodination of a stannyl oligodeoxyribonucleotide were undertaken to evaluate a gamma ray emitting ODN ligand for thrombus imaging in vivo . Synthesis of the ODN was based on modified automatedbeta-cyanoethyl phosphoramidite chemistry with an organotin nucleoside (dU*) coupled to a thrombin binding aptamer sequence to give d(U ...
H, Dougan, J B, Hobbs, J I, Weitz, D M, Lyster   +3 more
openaire   +2 more sources

A Solution to the Common Problem of the Synthesis and Applications of Hexachlorofluorescein Labeled Oligonucleotides. [PDF]

PLoS ONE, 2016
A common problem of the preparation of hexachlorofluorescein labeled oligonucleotides is the transformation of the fluorophore to an arylacridine derivative under standard ammonolysis conditions. We show here that the arylacridine byproduct with distinct
Andrey N Chuvilin, Igor P Smirnov, Alena G Mosina, Anna M Varizhuk, Galina E Pozmogova   +4 more
doaj   +1 more source

Synthetic Hexanucleotides as a Tool to Overcome Excessive Neutrophil Activation Caused by CpG-Containing Oligonucleotides

Pathogens, 2021
Mimicking bacterial DNA, synthetic CpG-containing oligodeoxyribonucleotides (CpG-ODNs) have a powerful immunomodulatory potential. Their practical application is mainly associated with the production of vaccines, where they are used as adjuvants, as well
Ekaterina A. Golenkina, Svetlana I. Galkina, Nina G. Dolinnaya, Evgenii A. Arifulin, Yulia M. Romanova, Galina F. Sud’ina   +5 more
doaj   +1 more source

Rainbow™ Universal CPG: A Versatile Solid Support for Oligonucleotide Synthesis

BioTechniques, 1997
We have developed a universal solid support, termed Rainbow™ Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore ...
P.S. Nelson, S. Muthini, M. Vierra, L. Acosta, T.H. Smith   +4 more
doaj   +1 more source

Phosphoryl guanidine oligonucleotides as primers for RNA-dependent DNA synthesis using murine leukemia virus reverse transcriptase

Вавиловский журнал генетики и селекции, 2022
Modern approaches to the detection and analysis of low-copy-number RNAs are often based on the use of RNA-dependent DNA polymerases, for example, in reverse-transcription PCR.
E. S. Dyudeeva, I. A. Pyshnaya
doaj   +1 more source

5′‐Iodination of Solid‐Phase‐Linked Oligodeoxyribonucleotides [PDF]

, 2003
5′‐Iodinated oligodeoxyribonucleotides readily react with 3′‐phosphorothioated DNA in the presence of a complementary template to yield a conjugate that is identical to natural DNA in every respect except that one oxygen atom in the phosphodiester ...
Gregory P. Miller, Miller, Gregory P., Eric T. Kool, Kool, Eric T.   +3 more
core   +1 more source

Determination of Methylation Site of DNA-Methyltransferase NlaX by a Hybrid Method

BioTechniques, 2002
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M·NlaX) from Neisseria lactamica was established to be
E.A. Kubareva, J. Walter, A.S. Karyagina, O.V. Vorob'eva, P.C.K. Lau, T. Trautner   +5 more
doaj   +1 more source