Results 11 to 20 of about 12,307 (190)

Frontiers and Approaches to Chemical Synthesis of Oligodeoxyribonucleotides

Molecules, 2013
The advantages and disadvantages of existing approaches to the synthesis of oligodeoxyribonucleotides (ODN) are discussed focusing on large-scale methods. The liquid phase and solid supported synthesis and the synthesis on soluble polymers are discussed.
Tatyana Abramova
doaj   +2 more sources

Biophysical and biological properties of quadruplex oligodeoxyribonucleotides [PDF]

Nucleic Acids Research, 2003
Single-stranded guanosine-rich oligodeoxyribonucleotides (GROs) have a propensity to form quadruplex structures that are stabilized by G-quartets. In addition to intense speculation about the role of G-quartet formation in vivo, there is considerable interest in the therapeutic potential of quadruplex oligonucleotides as aptamers or non-antisense ...
Virna, Dapić, Vedra, Abdomerović, Rachel, Marrington, Jemma, Peberdy, Alison, Rodger, John O, Trent, Paula J, Bates   +6 more
openaire   +3 more sources

Biochemical properties of phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides [PDF]

Nucleic Acids Research, 2003
Phosphorus-modified phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides were chemically synthesized and their biochemical properties evaluated. Under physiological pH, these DNA analogs possess negative charge and form stable, complementary A-like DNA:RNA heteroduplexes when analyzed via circular dichroism spectroscopy. Phosphonoacetate
David, Sheehan, Benjamin, Lunstad, Christina M, Yamada, Brian G, Stell, Marvin H, Caruthers, Douglas J, Dellinger   +5 more
openaire   +3 more sources

Use of a Dihydroxyacetone Derivative as Protecting Reagent to Phosphorylate Oligonucleotides. [PDF]

Curr Protoc
Abstract We present here a new reagent enabling the supported synthesis of oligodeoxynucleotides (ODNs) and oligoribonucleotides (ORNs) containing a phosphate group at the 5′‐terminal position after complete deprotection. This reagent, derived from a dihydroxyacetone core, contains a dimethoxytrityl (DMTr) group. The procedure for final deprotection is
Lartia R.
europepmc   +2 more sources

Synthetic Hexanucleotides as a Tool to Overcome Excessive Neutrophil Activation Caused by CpG-Containing Oligonucleotides

Pathogens, 2021
Mimicking bacterial DNA, synthetic CpG-containing oligodeoxyribonucleotides (CpG-ODNs) have a powerful immunomodulatory potential. Their practical application is mainly associated with the production of vaccines, where they are used as adjuvants, as well
Ekaterina A. Golenkina, Svetlana I. Galkina, Nina G. Dolinnaya, Evgenii A. Arifulin, Yulia M. Romanova, Galina F. Sud’ina   +5 more
doaj   +1 more source

Rainbow™ Universal CPG: A Versatile Solid Support for Oligonucleotide Synthesis

BioTechniques, 1997
We have developed a universal solid support, termed Rainbow™ Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore ...
P.S. Nelson, S. Muthini, M. Vierra, L. Acosta, T.H. Smith   +4 more
doaj   +1 more source

Position-Dependent Stabilization of DNA/RNA Duplexes by Site-Specific Incorporation of LNA Nucleosides. [PDF]

J Nucleic Acids
Owing to their enhanced functional properties, 2 ′,4 ′‐bridged nucleic acids/locked nucleic acids (2 ′,4 ′‐BNAs/LNAs) are considered promising candidates for antisense oligonucleotide therapeutics. The spatial arrangement of LNA residues within the oligonucleotide sequence is a key determinant of binding affinity to the target RNA.
Tomita-Sudo E, Akita T, Sakimoto N, Tahara-Takamine S, Sano R, Iwai S, Kawakami J.   +6 more
europepmc   +2 more sources

Phosphoryl guanidine oligonucleotides as primers for RNA-dependent DNA synthesis using murine leukemia virus reverse transcriptase

Вавиловский журнал генетики и селекции, 2022
Modern approaches to the detection and analysis of low-copy-number RNAs are often based on the use of RNA-dependent DNA polymerases, for example, in reverse-transcription PCR.
E. S. Dyudeeva, I. A. Pyshnaya
doaj   +1 more source

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Schwirzke, Marina, Kaluzab, Brigitte, Weissenhorn, Winfried, Weiss, Elisabeth H., Weidle, Ulrich H.   +4 more
core   +1 more source

Application of the weighted histogram method for calculating the thermodynamic parameters of the formation of oligodeoxyribonucleotide duplexes

Вавиловский журнал генетики и селекции, 2023
To date, many derivatives and analogs of nucleic acids (NAs) have been developed. Some of them have found uses in scientific research and biomedical applications. Their effective use is based on the data about their properties. Some of the most important
I. I. Yushin, V. M. Golyshev, D. V. Pyshnyi, A. A. Lomzov   +3 more
doaj   +1 more source