Results 31 to 40 of about 4,788 (197)
Beilstein Journal of Organic Chemistry, 2015 The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the building blocks, N-acyl protected
Amit M. Jabgunde +3 more
doaj +1 more source
PLoS Genetics, 2020 Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex
Thomas W van Ravesteyn +4 more
doaj +1 more source
An update on targeted gene repair in mammalian cells: methods and mechanisms
Journal of Biomedical Science, 2011 Transfer of full-length genes including regulatory elements has been the preferred gene therapy strategy for clinical applications. However, with significant drawbacks emerging, targeted gene alteration (TGA) has recently become a promising alternative ...
Bolund Lars +6 more
doaj +1 more source
, 2005 Toll-like receptor (TLR)-9 recognizes CpG motifs in microbial DNA. TLR9 signalling stimulates innate antimicrobial immunity and modulates adaptive immune responses including autoimmunity against chromatin, e.g., in systemic lupus erythematosus (SLE ...
Anders HJ, H-J Anders, Stacey KJ
core +1 more source
A Novel 3-Methyladenine DNA Glycosylase from Helicobacter pylori Defines a New Class within the Endonuclease III Family of Base Excision Repair Glycosylases [PDF]
, 2000 The cloning, purification, and characterization of MagIII, a 3-methyladenine DNA glycosylase from Helicobacter pylori, is presented in this paper. Sequence analysis of the genome of this pathogen failed to identify open reading frames potentially coding ...
Boiteux, Serge +5 more
core +1 more source
Sequence requirements for oligodeoxyribonucleotide inhibitory activity [PDF]
International Immunology, 2005 Inhibitory oligonucleotides (IN-ODN) differing from stimulatory CpG ODN (ST-ODN) by as few as two bases can block ST-ODN-induced proliferation, apoptosis protection and IL-6 secretion in B lymphocytes and the production of IL-12p40 by non-B cells. The main objective of this study was to determine the ODN sequence requirements for inhibition in mice ...
Robert F, Ashman +3 more
openaire +2 more sources
Reversible DNA micro-patterning using the fluorous effect [PDF]
, 2017 We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of ...
Burley, Glenn A. +7 more
core +1 more source
Synthesis and anti-HIV activity of thiocholesteryl-coupled phosphodiester antisense oligonucleotides incorporated into immunoliposomes [PDF]
, 1994 Encapsulation of oligonucleotides in antibody-targeted liposomes (immunoliposomes) which bind to target cells permits intracellular delivery of the oligonucleotides.
Akhtar +42 more
core +1 more source
Microwave-assisted rapid deprotection of oligodeoxyribonucleotides [PDF]
Nucleic Acids Research, 1997 A novel method for the deprotection of oligodeoxyribonucleotides under microwave irradiation has been developed. The oligodeoxynucleotides having base labile, phenoxyacetyl (pac), protection for exocyclic amino functions were fully deprotected in 0. 2 M sodium hydroxide (methanol:water : : 1:1, v/v) = A and 1 M sodium hydroxide (methanol:water : : 1:1,
P, Kumar, K C, Gupta
openaire +2 more sources
Convenient synthesis of arabinonucleoside containing oligodeoxyribonucleotides [PDF]
Nucleic Acids Symposium Series, 2000 C2 substituted arabinofuranosyluracil derivatives were synthesized and its incorporations into DNA were easily carried out by using post-synthetic modification.
H, Ozaki +3 more
openaire +2 more sources