Results 31 to 40 of about 11,432 (197)
Вавиловский журнал генетики и селекции, 2023 To date, many derivatives and analogs of nucleic acids (NAs) have been developed. Some of them have found uses in scientific research and biomedical applications. Their effective use is based on the data about their properties. Some of the most important
I. I. Yushin +3 more
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Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]
, 1991 We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Schwirzke, Marina +4 more
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Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides [PDF]
, 1993 Oligodeoxyribonucleotides containing a novel disulfide crosslink are shown to be amenable to chemical sequencing using standard sequencing reagents following reduction of the disulfide with DTT and selective alkylation of the free thiols with N ...
Gary D. Glick +3 more
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Вавиловский журнал генетики и селекции, 2022 The GRIN1, ASCL3, and NOS1 genes are associated with various phenotypes of neuropsychiatric disorders. For instance, these genes contribute to the development of schizophrenia, Alzheimer’s and Parkinson’s diseases, and epilepsy.
E. B. Sharypova +4 more
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Molecules, 2015 The rationale for the synthesis of cationic modified nucleosides is higher expected nuclease resistance and potentially better cellular uptake due to an overall reduced negative charge based on internal charge compensation.
Jolanta Brzezinska +1 more
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Transport Oligonucleotides—A Novel System for Intracellular Delivery of Antisense Therapeutics
Molecules, 2020 Biological activity of antisense oligonucleotides (asON), especially those with a neutral backbone, is often attenuated by poor cellular accumulation. In the present proof-of-concept study, we propose a novel delivery system for asONs which implies the ...
Oleg V. Markov +9 more
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Beilstein Journal of Organic Chemistry, 2015 The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the building blocks, N-acyl protected
Amit M. Jabgunde +3 more
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PLoS Genetics, 2020 Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex
Thomas W van Ravesteyn +4 more
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An update on targeted gene repair in mammalian cells: methods and mechanisms
Journal of Biomedical Science, 2011 Transfer of full-length genes including regulatory elements has been the preferred gene therapy strategy for clinical applications. However, with significant drawbacks emerging, targeted gene alteration (TGA) has recently become a promising alternative ...
Bolund Lars +6 more
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Sequence requirements for oligodeoxyribonucleotide inhibitory activity [PDF]
International Immunology, 2005 Inhibitory oligonucleotides (IN-ODN) differing from stimulatory CpG ODN (ST-ODN) by as few as two bases can block ST-ODN-induced proliferation, apoptosis protection and IL-6 secretion in B lymphocytes and the production of IL-12p40 by non-B cells. The main objective of this study was to determine the ODN sequence requirements for inhibition in mice ...
Robert F, Ashman +3 more
openaire +2 more sources