Results 51 to 60 of about 12,307 (190)

Inhibition of deoxyribonucleases by phosphorothioate groups in oligodeoxyribonucleotides

Nucleic Acids Research, 1988
The Rp- and Sp-diastereomers of the phosphorothioate-containing oligonucleotide d[ApAp(S)ApA] have been synthesized. They and the tetramer d[ApApApA] were tested as substrates for staphylococcal nuclease, DNase II and spleen phosphodiesterase. For digestions with DNase I these oligonucleotides were converted to the 5'-phosphorylated derivates.
S, Spitzer, F, Eckstein
openaire   +3 more sources

A new method for sequence analysis of oligodeoxyribonucleotides [PDF]

Nucleic Acids Research, 1985
The structure of an oligodeoxyribonucleotide may be determined by a simple two-dimensional separation on a polyethyleneimine-cellulose thin layer sheet. Chromatography in the first dimension fractionates by chain length a nested set of fragments that are generated by subjecting the oligomer to partial spleen phosphodiesterase degradation and then ...
D M, Black, P T, Gilham
openaire   +2 more sources

Cas9‐ and Cas12a‐mediated excision and replacement of the celiac disease‐related α‐gliadin immunogenic complex in hexaploid wheat

Plant Biotechnology Journal, Volume 23, Issue 9, Page 3798-3813, September 2025.
Summary Celiac disease (CD) is a chronic enteropathy affecting approximately 1% of the global population. Wheat α‐gliadins are a major contributor to the autoimmune response, as they contain one of the most immunogenic peptides, the 33‐mer, along with numerous variants.
Miriam Marín‐Sanz, Susana Sánchez‐León, María H. Guzmán‐López, Colby G. Starker, Daniel F. Voytas, Francisco Barro   +5 more
wiley   +1 more source

Fast Deprotection of Synthetic Oligodeoxyribonucleotides Using Standard Reagents under Microwave Irradiation

, 2008
Fast methods for the removal of permanent amide exo-cyclic protective groups widely used in phosphoramidite- method DNA synthesis are desirable for many genomics and proteomics applications. In this communication, we present a method for the deprotection
LaFlamme, Mark, Culf, A.S., Cuperlovic-Culf, Miroslava, Ouellette, Rodney J., Tardiff, Bennett J.   +4 more
core   +1 more source

The Comprehensive Roadmap Toward Malaria Elimination Using Graphene and its Promising 2D Analogs

Advanced NanoBiomed Research, Volume 5, Issue 8, August 2025.
Material based on a diversity of graphene with chemical properties of strong strength, high mobility, high transparency, good heat conductivity, biocompatibility, and chemical stability, advanced devices demonstrating antimalarial characteristics can be used for malaria treatment at different stages of malaria development from the injection of ...
Fangzhou He, George Junior, Rajashree Konar, Yuanding Huang, Ke Zhang, Lijing Ke, Meng Niu, Boon Tong Goh, Amine El Moutaouakil, Gilbert Daniel Nessim, Mohamed Belmoubarik, Weng Kung Peng   +11 more
wiley   +1 more source

Antibodies elicited by defined oligodeoxyribonucleotide sequences

Nucleic Acids Research, 1978
Antibodies to the oligodeoxyribonucleotides d(pT)3, d(pT)4, d(pT)6 and d(pA-A-T-T) were elicited in rabbits by immunization with electrostatic complexes of the respective haptens with methylated bovine serum albumin (MBSA). The antisera were assayed by complement fixation using denatured DNA's of various sources as antigens.
H J, Störl, H, Simon, H, Barthelmes
openaire   +3 more sources

Immobilization of oligodeoxyribonucleotides with multiple anchors to microchips [PDF]

Nucleic Acids Research, 2001
Facile modification of oligodeoxyribonucleotides is required for efficient immobilization to a pre-activated glass surface. This report presents an oligodeoxyribonucleotide which contains a hairpin stem-loop structure with multiple phosphorothioate moieties in the loop.
X, Zhao, S, Nampalli, A J, Serino, S, Kumar   +3 more
openaire   +2 more sources

An engineered redox‐switchable streptavidin mutein enables the high‐affinity capture and efficient elution of biotinylated ligands

Protein Science, Volume 34, Issue 8, August 2025.
Abstract Although the high affinity and specificity of the interaction between streptavidin and biotin are widely utilized to isolate biotinylated ligands, their elution over practical timescales requires the use of strongly denaturing conditions that are incompatible with many target biomolecules.
Jesse M. Marangoni, Adriana Zutic, Amrita Dhindsa, Emmanuella Uche‐Orji, Sui‐Lam Wong, Kenneth K. S. Ng   +5 more
wiley   +1 more source

Chemical Syntheses of Oligodeoxyribonucleotides Containing Spore Photoproduct

, 2016
5-(α-Thyminyl)-5,6-dihydrothymine, also called spore photoproduct or SP, is commonly found in the genomic DNA of UV-irradiated bacterial endospores. Despite the fact that SP was discovered nearly 50 years ago, its biochemical impact is still largely ...
Yajun Jian (1321638), Lei Li (29537)
core   +1 more source

The chemical end-ligation of homopyrimidine oligodeoxyribonucleotides within a DNA triple helix [PDF]

, 1997
Background: Triple-helical nucleic acids, first reported in the late 1950s, are receiving attention for their possible involvement in controlling gene expression. Certain sequences of DNA are believed to form local triple-helical structures (H-form DNA),
Li, Tianhu, Nicolaou, K.C., Weinstein, David S.   +2 more
core   +1 more source