Results 61 to 70 of about 98,363 (283)

Recombining your way out of trouble: the genetic architecture of hybrid fitness under environmental stress [PDF]

, 2020
Hybridization between species is a fundamental evolutionary force that can both promote and delay adaptation. There is a deficit in our understanding of the genetic basis of hybrid fitness, especially in non-domesticated organisms.
Bendixsen, D., Greig, D., Janzen, T., Nolte, A., Stelkens, R., Zhang, Z.   +5 more
core   +1 more source

Intact DNA purified from flow-sorted nuclei unlocks the potential of next-generation genome mapping and assembly in Solanum species

MethodsX, 2018
Next-generation genome mapping through nanochannels (Bionano optical mapping) of plant genomes brings genome assemblies to the ‘nearly-finished’ level for reliable and detailed gene annotations and assessment of structural variations.
Paola Gaiero, Hana Šimková, Jan Vrána, Federico F. Santiñaque, Beatriz López-Carro, Gustavo A. Folle, José van de Belt, Sander A. Peters, Jaroslav Doležel, Hans de Jong   +9 more
doaj   +1 more source

De novo assembly of the cattle reference genome with single-molecule sequencing. [PDF]

, 2020
BackgroundMajor advances in selection progress for cattle have been made following the introduction of genomic tools over the past 10-12 years. These tools depend upon the Bos taurus reference genome (UMD3.1.1), which was created using now-outdated ...
Bickhart, Derek M, Cole, John B, Couldrey, Christine, Dreischer, Christian, Elsik, Christine G, Ghurye, Jay, Hagen, Darren E, Hall, Richard, Hammond, John A, Hoffman, Jinna, Koren, Sergey, Li, Wenli, Liu, George, Low, Wai Y, McDaneld, Tara G, McKay, Stephanie D, Medrano, Juan F, Murdoch, Brenda M, Nandolo, Wilson, Phillippy, Adam M, Rhie, Arang, Rosen, Benjamin D, Rowan, Troy N, Schnabel, Robert D, Schroeder, Steven G, Schultheiss, Sebastian J, Schwartz, John C, Smith, Timothy PL, Snelling, Warren M, Thibaud-Nissen, Françoise, Tseng, Elizabeth, Van Tassell, Curtis P, Zimin, Aleksey   +32 more
core  

REAPR: a universal tool for genome assembly evaluation. [PDF]

, 2013
Methods to reliably assess the accuracy of genome sequence data are lacking. Currently completeness is only described qualitatively and mis-assemblies are overlooked.
Berriman, Matthew, Hunt, Martin, Kikuchi, Taisei, Newbold, Chris, Otto, Thomas D, Sanders, Mandy   +5 more
core   +2 more sources

Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer

Molecular Oncology, EarlyView.
PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta, Yang Song, Madeleine Ndahayo, Anirudh Saxena, Theresa Guo, Dylan Z. Kelley, Jessica Gore, Andrew Hennigan, Alexa Anderson, John C. Papadimitriou, Daria A. Gaykalova   +10 more
wiley   +1 more source

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing. [PDF]

, 2018
Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of cellular heterogeneity in biological systems.
Abate, Adam R, Demaree, Benjamin, Lan, Freeman, Weisgerber, Daniel   +3 more
core   +2 more sources

Targeted modulation of IGFL2‐AS1 reveals its translational potential in cervical adenocarcinoma

Molecular Oncology, EarlyView.
Cervical adenocarcinoma patients face worse outcomes than squamous cell carcinoma counterparts despite similar treatment. The identification of IGFL2‐AS1's differential expression provides a molecular basis for distinguishing these histotypes, paving the way for personalized therapies and improved survival in vulnerable populations globally.
Ricardo Cesar Cintra, Lucca Paolo Hsu Helmich, Daniel Rodrigues de Bastos, Laura Sichero, Patrícia Savio de Araujo‐Souza, Fabio Passetti, Luisa Lina Villa   +6 more
wiley   +1 more source

Optical Genome Mapping Reveals Genomic Alterations upon Gene Editing in hiPSCs: Implications for Neural Tissue Differentiation and Brain Organoid Research

Cells
Genome editing, notably CRISPR (cluster regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9), has revolutionized genetic engineering allowing for precise targeted modifications.
Lucia Gallego Villarejo, Wanda M. Gerding, Lisa Bachmann, Luzie H. I. Hardt, Stefan Bormann, Huu Phuc Nguyen, Thorsten Müller   +6 more
doaj   +1 more source

Utility of Optical Genome Mapping in Repeat Disorders

Clinical Genetics
ABSTRACT Genomic repeat sequences are patterns of nucleic acids that exist in multiple copies throughout the genome. More than 60 Mendelian disorders are caused by the expansion or contraction of these repeats. Various specific methods for determining tandem repeat variations have been developed.
Mehmet Burak Mutlu, Taner Karakaya, Hamide Betül Gerik Çelebi, Fahrettin Duymuş, Serhat Seyhan, Sanem Yılmaz, Uluç Yiş, Tahir Atik, Mehmet Fatih Yetkin, Hakan Gümüş   +9 more
openaire   +6 more sources

BiSCoT: Improving large eukaryotic genome assemblies with optical maps

PeerJ, 2019
ABSTRACTMotivationLong read sequencing and Bionano Genomics optical maps are two techniques that, when used together, make it possible to reconstruct entire chromosome or chromosome arms structure. However, the existing tools are often too conservative and organization of contigs into scaffolds is not always optimal.ResultsWe developed BiSCoT (Bionano ...
Istace, Benjamin, Belser, Caroline, Aury, Jean-Marc   +2 more
openaire   +3 more sources