Results 41 to 50 of about 1,425,864 (268)

The dynamics of replication licensing in live Caenorhabditis elegans embryos [PDF]

, 2012
Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of ...
Blow, J. Julian; id_orcid, Remi Sonneville, Matthieu Querenet, Sonneville, Remi, J. Julian Blow, Anton Gartner, Querenet, Matthieu, Blow, J. Julian, Gartner, Anton, Craig, Ashley, Ashley Craig   +10 more
core   +1 more source

Nucleosomes influence multiple steps during replication initiation

eLife, 2017
Eukaryotic replication origin licensing, activation and timing are influenced by chromatin but a mechanistic understanding is lacking. Using reconstituted nucleosomal DNA replication assays, we assessed the impact of nucleosomes on replication initiation.
Ishara F Azmi, Shinya Watanabe, Michael F Maloney, Sukhyun Kang, Jason A Belsky, David M MacAlpine, Craig L Peterson, Stephen P Bell   +7 more
doaj   +1 more source

DNA replication licensing in somatic and germ cells [PDF]

, 2004
The DNA replication (or origin) licensing system ensures precise duplication of the genome in each cell cycle and is a powerful regulator of cell proliferation in metazoa.
Williams, C, Jung, Hyo-Il, Obermann, EC, Ellen C. Obermann, Kai Stoeber, Loddo, Marco, Kathryn Leigh Eward, Marco Loddo, Shreeram, S, Williams, GH, Obermann, Ellen C, Hyo-Il Jung, Williams, Craig, J. Julian Blow, Gareth H. Williams, Eward, Kathryn Leigh, Fanshawe, Thomas, Jung, HI, Shreeram, S., Prevost, Toby; id_orcid, Blow, J. Julian; id_orcid, A. Toby Prevost, Loddo, M, Williams, Gareth H., Thomas Fanshawe, Fanshawe, T, Obermann, Ellen C., Eward, KL, Williams, Gareth H, Jung, H-I, S. Shreeram, Blow, J Julian, Prevost, A. Toby, Stoeber, K, Stoeber, Kai, Blow, J. Julian, Prevost, AT, Blow, JJ, Craig Williams   +38 more
core   +1 more source

Does Licensing Resolve Hold Up in the Patent Thicket? [PDF]

, 2008
In a patent thicket licensing provides a mechanism to either avoid or resolve hold up. We study the choice between ex ante licensing to avoid hold up and ex post licensing to resolve it.
von Graevenitz, Georg, Graevenitz, Georg von, Siebert, Ralph   +2 more
core   +1 more source

An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Cell Reports, 2020
Summary: Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle.
Aftab Amin, Rentian Wu, Man Hei Cheung, John F. Scott, Ziyi Wang, Zijing Zhou, Changdong Liu, Guang Zhu, Chris Kong-Chu Wong, Zhiling Yu, Chun Liang   +10 more
doaj   +1 more source

Socio-Environmental Conflicts: A Study on the Logistic Center in Paranapiacaba and Involved Social Representations

Ambiente & Sociedade, 2021
Socio-environmental conflicts have been intensifying in the 21st century, which has prompted new approaches seeking to identify the origin of these conflicts and enrich their analyses.
Virgínia Hamer Campagnaro, Luciana Aparecida Farias, Giovano Candiani   +2 more
doaj   +2 more sources

A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding

eLife, 2019
Licensing of eukaryotic origins of replication requires DNA loading of two copies of the Mcm2-7 replicative helicase to form a head-to-head double-hexamer, ensuring activated helicases depart the origin bidirectionally.
Kanokwan Champasa, Caitlin Blank, Larry J Friedman, Jeff Gelles, Stephen P Bell   +4 more
doaj   +1 more source

Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones

eLife, 2021
Eukaryotic DNA replication initiates during S phase from origins that have been licensed in the preceding G1 phase. Here, we compare ChIP-seq profiles of the licensing factors Orc2, Orc3, Mcm3, and Mcm7 with gene expression, replication timing, and fork ...
Nina Kirstein, Alexander Buschle, Xia Wu, Stefan Krebs, Helmut Blum, Elisabeth Kremmer, Ina M Vorberg, Wolfgang Hammerschmidt, Laurent Lacroix, Olivier Hyrien, Benjamin Audit, Aloys Schepers   +11 more
doaj   +1 more source

Dormant Origins, the Licensing Checkpoint, and the Response to Replicative Stresses [PDF]

Cold Spring Harbor Perspectives in Biology, 2012
Only ∼10% of replication origins that are licensed by loading minichromosome maintenance 2-7 (MCM2-7) complexes are normally used, with the majority remaining dormant. If replication fork progression is inhibited, nearby dormant origins initiate to ensure that all of the chromosomal DNA is replicated.
McIntosh, Debbie, Blow, J. Julian
openaire   +3 more sources

Cryo-EM structure of a licensed DNA replication origin [PDF]

Nature Communications, 2017
AbstractEukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase.
Ferdos Abid Ali, Max E. Douglas, Julia Locke, Valerie E. Pye, Andrea Nans, John F. X. Diffley, Alessandro Costa   +6 more
openaire   +3 more sources