Results 121 to 130 of about 2,721 (157)
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Antibodies to orthopoxvirus in domestic cats in Norway
Veterinary Record, 1998The prevalence of antibodies to orthopoxvirus in 217 sera collected from domestic cats in the western part of Norway was 10.1 per cent as measured by a competitive ELISA. In one of the seropositive cats antibodies were also detected by an immunofluorescence assay.
M, Tryland +5 more
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Inactivation of orthopoxvirus for diagnostic PCR analysis
Journal of Virological Methods, 2007Diagnoses of ongoing viral infections commonly rely on PCR methodology. Sample material that may contain hazardous virus should be efficiently inactivated in biological containment or bed-side before diagnostic PCR analysis. Surprisingly little documentation is available for inactivation of human viral pathogens by inactivation reagents that allow for ...
Lasse, Vinner, Anders, Fomsgaard
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Orthopoxvirus pan-genomic DNA assay
Journal of Virological Methods, 2007A genome-spanning assay is described that enables laboratory confirmation of infections with orthopoxviruses (OPVs), particularly Vaccinia, Monkeypox, and Variola viruses, which can cause vesiculo-pustular rash illnesses in humans. The assay uses a series of polymerase chain reaction (PCR) amplicons that overlap to span the approximately 200kilobase ...
Yu, Li +4 more
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Two types of deletions in orthopoxvirus genomes
Virus Genes, 1995The genome nucleotide sequences of two strains of variola major virus and one strain of vaccinia virus were compared. One hundred and sixty-eight short (less than 100 bp in length) and eight long (more than 900 bp in length) deletions, four deletion/insertion regions, and four regions of multiple mutational differences between variola and vaccinia ...
S N, Shchelkunov, A V, Totmenin
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Orthopoxvirus Purification for High Containment Laboratories
Purification of large viruses in a high containment laboratory can create unique challenges. Traditional purification methods for large viruses rely on equipment and techniques that are not ideal for high containment work. Poxvirus purification has long relied on the use of Genetron®, a reagent that is no longer available.Laura, Hughes +4 more
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Orthopoxvirus Genome Sequencing, Assembly, and Analysis
Poxviruses have exceptionally large genomes compared to most other viruses, which represent unique challenges to sequencing and assembly due to complex features such as repeat elements and low complexity sequences. The 2022 global mpox outbreak led to an unprecedented level of poxvirus sequencing as public health and research institutions faced with ...Crystal M, Gigante +2 more
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Mécanismes d'échappement immunitaire des Orthopoxvirus Orthopoxvirus immune evasion mechanism
Médecine et Maladies Infectieuses, 2004F Messud-Petit, S Bertagnoli
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Genus Orthopoxvirus: Variola virus
2007Variola major virus caused the human disease smallpox; interpretations of the historic record indicate that the initial introduction of disease in a naive population had profound effects on its demographics. Smallpox was declared eradicated by the World Health Organization (WHO) in 1980.
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