Results 61 to 70 of about 1,506,122 (305)

Histamine-H1-receptor-mediated phosphoinositide hydrolysis, Ca2+ signalling and membrane-potential oscillations in human HeLa carcinoma cells [PDF]

Biochemical Journal, 1990
In human HeLa carcinoma cells, histamine causes a dose-dependent formation of inositol phosphates, production of diacylglycerol and a transient rise in intracellular [Ca2+]. These responses are completely blocked by the H1-receptor antagonist pyrilamine.
B C, Tilly, L G, Tertoolen, A C, Lambrechts, R, Remorie, S W, de Laat, W H, Moolenaar   +5 more
openaire   +2 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Oscillating on Borrowed Time: Diffusible Signals from Immortalized Suprachiasmatic Nucleus Cells Regulate Circadian Rhythmicity in Cultured Fibroblasts [PDF]

The Journal of Neuroscience, 2001
The capacity to generate circadian rhythms endogenously and to confer this rhythmicity to other cells was compared in immortalized cells derived from the suprachiasmatic nucleus (SCN) and a fibroblast line to differentiate SCN pacemaker properties from the oscillatory behavior of non-clock tissues.
G, Allen, J, Rappe, D J, Earnest, V M, Cassone   +3 more
openaire   +2 more sources

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Remodeling of calcium signaling and store operated calcium entry as a consequence of human neural progenitor cell differentiation

Scientific Reports
The differentiation of neural progenitor cells into glial and neuronal lineages is a fundamental process in neurodevelopment. This complex process is tightly regulated by several signaling pathways, including calcium signaling. Calcium signaling not only
Silke B. Chalmers, Mélanie Robitaille, Francisco Sadras, Sarah J. Roberts-Thomson, Gregory R. Monteith   +4 more
doaj   +1 more source

Repetitive mitochondrial Ca 2+ signals synchronize with cytosolic Ca 2+ oscillations in the pancreatic beta-cell line, MIN6 [PDF]

Diabetologia, 1998
We examined the relationship between cytosolic Ca2+ concentration ([Ca2+]c) and mitochondrial matrix Ca2+ concentration ([Ca2+]m) in the pancreatic beta-cell line, MIN6. [Ca2+]c was monitored in a single or a group (30 cells) of fura-2-loaded MIN6 cells, and [Ca2+]m was measured in a group (1 x 10[6] cells) of MIN6 cells stably transfected with ...
M, Nakazaki, H, Ishihara, M, Kakei, K, Inukai, T, Asano, J I, Miyazaki, H, Tanaka, M, Kikuchi, T, Yada, Y, Oka   +9 more
openaire   +2 more sources

Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.

Cell, 2018
Recent reports indicate that hypoxia influences the circadian clock through the transcriptional activities of hypoxia-inducible factors (HIFs) at clock genes. Unexpectedly, we uncover a profound disruption of the circadian clock and diurnal transcriptome
Z. Walton, Chirag H. Patel, Rebekah C. Brooks, Yongjun Yu, Arig Ibrahim-Hashim, Malini Riddle, A. Porcu, Tianying Jiang, B. Ecker, Feven Tameire, C. Koumenis, A. Weeraratna, D. Welsh, R. Gillies, J. Alwine, Lin Zhang, J. Powell, C. Dang   +17 more
semanticscholar   +1 more source

Visual Recovery Reflects Cortical MeCP2 Sensitivity in Rett Syndrome

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Rett syndrome (RTT) is a devastating neurodevelopmental disorder with developmental regression affecting motor, sensory, and cognitive functions. Sensory disruptions contribute to the complex behavioral and cognitive difficulties and represent an important target for therapeutic interventions.
Alex Joseph Simon, Nathalie Picard, Valeria d'Andrea, Enchi Chang, Joseph Leffler, Eleonora Centofante, Matthew Taylor, Francesca Bardi, Francesca Cavicchiolo, Takao K. Hensch, Stefano Panzeri, Chinfei Chen, Michela Fagiolini   +12 more
wiley   +1 more source

Cross-talk between signaling pathways can generate robust oscillations in calcium and cAMP. [PDF]

PLoS ONE, 2009
BACKGROUND:To control and manipulate cellular signaling, we need to understand cellular strategies for information transfer, integration, and decision-making.
Fernando Siso-Nadal, Jeffrey J Fox, Stéphane A Laporte, Terence E Hébert, Peter S Swain   +4 more
doaj   +1 more source

Regulation of molecular clock oscillations and phagocytic activity via muscarinic Ca2+ signaling in human retinal pigment epithelial cells [PDF]

Scientific Reports, 2017
AbstractVertebrate eyes are known to contain circadian clocks, however, the intracellular mechanisms regulating the retinal clockwork remain largely unknown. To address this, we generated a cell line (hRPE-YC) from human retinal pigmental epithelium, which stably co-expressed reporters for molecular clock oscillations (Bmal1-luciferase) and ...
Rina Ikarashi, Honami Akechi, Yuzuki Kanda, Alsawaf Ahmad, Kouhei Takeuchi, Eri Morioka, Takashi Sugiyama, Takashi Ebisawa, Masaaki Ikeda, Masayuki Ikeda   +9 more
openaire   +2 more sources