Results 141 to 150 of about 33,843 (263)

High-Coverage Long Read DNA Sequencing with the Oxford Nanopore MinION [PDF]

, 2017
Nanopore sequencing was conceived in 1989 by Dave Deamer (UCSC). Over two decades of development from research laboratories and, later on, Oxford Nanopore Technologies resulted in the MinION nanopore sequencer.
Jain, Miten
core  

Dissecting the genetic basis of climatic adaptation in wild relatives (Malus baccata) for climate‐resilient apple breeding

Journal of Integrative Plant Biology, EarlyView.
This comprehensive map of genetic variation in wild apple (Malus baccata) and set of key genes associated with adaptation to temperature, precipitation, and soil properties establishes a foundation for breeding climate‐resilient apple cultivars and conserving the genetic resources of wild crop relatives.
Ying Su, Yani Hao, Xuejing Cao, Lin Wang, Zhiqi Xu, Fan Zhang, Zhiyao Ma, Xu Wang, Jiacui Li, Tianrong Fan, Ruoyan Zhao, Zhongqi Liu, Wenrui Wang, Yingchun Zhang, Xuanwen Yang, Sifan Yang, Dajiang Wang, Kun Wang, Simiao Sun, Zichen Li, Wen Tian, Yanming Sun, Zhao Liu, Yanshuai Xu, Hua Xiao, Yanling Peng, Xiaodong Xu, Ruirui Liu, Xinmin Tian, Yongfeng Zhou, Yuan Gao   +30 more
wiley   +1 more source

A Comprehensive DNA Barcode Reference Library for the Macroinvertebrates of Scottish Seagrass Beds Using Oxford Nanopore Flongle Flowcells

Ecology and Evolution
Oxford Nanopore Sequencing Technology (ONT) has emerged as a scalable method for generating DNA barcode reference libraries, capable of sequencing hundreds of DNA barcodes simultaneously using the portable, benchtop MinION sequencing device.
E. G. Ross, S. B. Piertney, J. D. Sigwart, N. F. Crook, A. Moreau, K. K. S. Layton   +5 more
doaj   +1 more source

A unique sessile loricate euglenid, Lepocinclis loricata sp. nov. (Euglenophyta, Phacaceae), from South Africa: Evolutionary implications

Journal of Phycology, EarlyView.
Abstract In this paper, we describe Lepocinclis loricata sp. nov. (Phacaceae), a sessile loricate euglenid that differs from all known loricate taxa by its unique combination of the presence of a lorica, monad morphology, sessile habit, and phylogenetic position within the Phacaceae. Populations of this species inhabit two turbid, silt‐rich waterbodies,
Bożena Zakryś, Sanet Janse van Vuuren, Aleksandra Kluczek, Maja Łukomska‐Kowalczyk   +3 more
wiley   +1 more source

Pharmacokinetics of Long‐Acting Ampicillin and Its Impact on the Gut Microbiome and Resistome in Rhesus Macaques (Macaca mulatta)

Journal of Veterinary Pharmacology and Therapeutics, EarlyView.
ABSTRACT Injectable antimicrobials with documented prolonged dosing intervals (> 48 h) for use in rhesus macaques are sparse. The objective of our study was to assess the pharmacokinetics, urine excretion, and effects on gut microbiome and resistome of intramuscular administered long‐acting formulation of ampicillin (Albipen LA) in macaques.
Annemiek Maaskant, Ingeborg M. van Geijlswijk, Mathias Devreese, Marc Cherlet, Leo F. van Geest, Jaap A. Wagenaar, Jan A. M. Langermans, Aldert L. Zomer, Jaco Bakker, Ronette Gehring   +9 more
wiley   +1 more source

O52: Diagnostic benefits of long-read genome sequencing for short-read negative rare disease cases

Genetics in Medicine Open
Alan Beggs, Tina Truong, Wanqing Shao, Bryan Leland, Kshitij Amar, Mehmet Bugrahan Duz, Nathaniel Edisis, Casie Genetti, Médéric Jeanne, Pratiksha Pradhan, Priyesh Rughani, Luca Schierbaum, Shirlee Shril, Rebecca Stubbs, Malika Sud, Kenney Tuyen, Carly Tyer, Talia Tzadikario, Scott Hickey, Sissel Juul, Piotr Sliz, Monica Wojcik, Friedhelm Hildebrandt, Catherine Brownstein, Miten Jain, Wendy Chung   +25 more
doaj   +1 more source

A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing

Medical and Veterinary Entomology, EarlyView.
Insectary‐reared mosquitoes were simultaneously assessed for species identification, bloodmeal source analysis and Plasmodium detection, using a single multiplexed sequencing run on the MinION sequencing platform. The method was applied to 150 insectary‐reared mosquitoes, representing nine species blood‐fed with five vertebrate hosts and 40 samples for
E. Abby Rogers, Dieunel Derilus, Christopher Sandi, Lisa Reimer, Audrey Lenhart, Lucy Mackenzie Impoinvil   +5 more
wiley   +1 more source

Fine‐tuning quantitative agronomic traits by manipulating gene copy number in rice

New Phytologist, EarlyView.
Although plant pan‐genome studies have revealed extensive copy number variations, their phenotypic consequences remain poorly understood. Here, we manipulated the copy number of OsMADS18 in rice (Oryza sativa) cv ‘Hitomebore’ using the CRISPR/Cas9 system.
Chihiro Nomura, Hiroyuki Kanzaki, Eiko Kanzaki, Motoki Shimizu, Kaori Oikawa, Hiroe Utsushi, Kazue Ito, Yusaku Sugimura, Ryohei Terauchi, Akira Abe   +9 more
wiley   +1 more source

Deep Learning Algorithms for Variant Calling in Oxford Nanopore Sequencing

, 2019
The accurate identification of DNA sequence variants is an important, but challenging task in genomics. It is particularly difficult for single molecule sequencing, which has a per- nucleotide error rate of ~5–15%.
Luo, R
core  

Long‐term surveillance reveals hybridization by nuclear reassortment and intercontinental spread as major evolutionary drivers in wheat yellow rust

New Phytologist, EarlyView.
Documented incursions of Puccinia striiformis 2009–2023 based on sequential resampling of MLGs/pathogen races on different continents. Summary Evolutionary forces affecting crop pathogens, including hybridization and long‐distance dispersal (LDD), may have strong implications for food security and sustainable plant disease control at global scales ...
Mogens Støvring Hovmøller, Tine Thach, Julian Rodriguez‐Algaba, Jens Grønbech Hansen, Marcel Meyer, David P. Hodson, Kumarse Nazari, Robert F. Park, Rita Tam, Mareike Möller, Benjamin Schwessinger, John P. Rathjen, Paula Silva, Venancio Riella, Annemarie Fejer Justesen   +14 more
wiley   +1 more source