Results 121 to 130 of about 5,800,592 (273)

Characterization of the dynamic microbiome evolution across thrips species

Insect Science, EarlyView.
Comprehensive survey of the microbiome in thrips. The dominant bacterial genera found in thrips include intracellular ones, such as Wolbachia and Spiroplasma, and extracellular ones, including Serratia, Pantoea, and Acinetobacter. We isolated and sequenced high‐quality genomes of two dominant symbionts, Pantoea dispersa and Serratia marcescens.
Xiaodi Hu, Zhaohui Shi, Yangwei Gao, Hanze Zheng, Lin Lin, Jian‐Ping Chen, Yong Chen, Chuan‐Xi Zhang, Yiyuan Li   +8 more
wiley   +1 more source

Decoding Dual Regulatory Layers: Integrating Differential Expression and Alternative Splicing Dynamics in the Evolution of Laryngeal Echolocation Across Chiropteran Lineages

Integrative Zoology, EarlyView.
We integrated short‐read and long‐read RNA‐seq data from cochlear tissues of echolocating and non‐echolocating bats to disentangle transcriptional and post‐transcriptional regulation. Echolocating bats showed neural‐function enrichment among differentially expressed genes, while alternatively spliced genes were linked to epigenetic regulation. Overlaps
Jianyu Wu, Daoyuan Hu, Min Chen, Xiuguang Mao   +3 more
wiley   +1 more source

Reproducibility and accuracy of bacterial methylome profiling using Oxford Nanopore Technologies nanopore sequencing platform

Microbial Genomics
Background. Oxford Nanopore Technologies (ONT) enables direct methylome profiling using the Dorado basecaller, but the comparative performance of ONT-only versus hybrid-assembly reference-based methylation calling, particularly regarding inter-operator variability, remains understudied.
Taylor Schababerle, Omar Hayat, Jaeone Jung, Minh Le, Isabella Polic, Hanan Wees, Micah Bhatti, Samuel Shelburne, Xiaojun Liu, Awdhesh Kalia   +9 more
openaire   +2 more sources

Evolutionary and Epidemiological Characterization of Multidrug‐Resistant Escherichia coli Isolated From Pangolins in China

Integrative Zoology, EarlyView.
ABSTRACT Malayan pangolin populations have fallen to a critically endangered level, and illegal trafficking of wild pangolins is a global problem. Little is known about antimicrobial‐resistant bacteria (MDRB) harbored by pangolins, although pangolins are frequently consumed as food or used in traditional medicine. In this study, we report for the first
Yawen Wang, Xue Wang, Jianan Zhao, Jun Zeng, Shijia Hu, Fang Ji, Guoying Dong, Hongxuan He, Chengmin Wang   +8 more
wiley   +1 more source

NanoR: A user-friendly R package to analyze and compare nanopore sequencing data.

PLoS ONE, 2019
MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and ...
Davide Bolognini, Niccolò Bartalucci, Alessandra Mingrino, Alessandro Maria Vannucchi, Alberto Magi   +4 more
doaj   +1 more source

Microbiome Insights Into Zoonotic Risk at Wildlife–Human Interfaces in a Transitioning Landscape in Thailand

Integrative Zoology, EarlyView.
This study shows how ecological transitions, such as reforestation in Southeast Asia, reshape host–microbe–pathogen dynamics at the wildlife–domestic interface. By integrating long‐read microbiome sequencing, ecological gradients, and statistical and network analysis, we identify host species that disproportionately contribute to pathogen diversity and
Pauline Van Leeuwen, Chuanpot Thinphovong, Vincent Sluydts, Alix Nicolas, Anamika Kritiyakan, Areeya Kriengudom, Pipat Soisook, Kittipong Chaisiri, Serge Morand, Johan Michaux   +9 more
wiley   +1 more source

Oxford Nanopore Technologies: long-read sequencing for the enhancement of diagnostic analysis of rare monogenic diseases

openLa diagnosi di malattie monogeniche rare ad oggi rimane spesso incompleta a causa delle limitazioni delle tecniche di sequenziamento short-read. Pertanto, una soluzione possibile è l’utilizzo in ambito diagnostico di tecniche di sequenziamento long-
DALL'ARMI, ELENA
core  

de novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinION sequencer

, 2017
International audienceBackground: Oxford Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single-molecule nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter ...
Cruaud, Corinne, Stefan Engelen, Odette Beluche, Anne Friedrich, Payen, Emilie, Liti, Gianni, Wincker, Patrick, Lemainque, Arnaud, Caradec, Claudia, Aury, Jean-Marc, Sébastien Faye, Benjamin Istace, Engelen, Stefan, Schacherer, Joseph, Faye, Sébastien, Corinne Cruaud, Patrick Wincker, d'Agata, Léo, Claudia Caradec, Joseph Schacherer, Gianni Liti, Friedrich, Anne, Arnaud Lemainque, Beluche, Odette, Davidas, Sabrina, Léo d'Agata, Emilie Payen, Istace, Benjamin, Sabrina Davidas, Jean-Marc Aury   +29 more
core   +1 more source

Reliable investigation of DNA methylation using Oxford nanopore technologies

Scientific Reports
Oxford Nanopore Technologies (ONT) offers an efficient and effective solution for studying DNA methylation. Both R9.4.1 and R10.4.1 ONT chemistries have been widely used to generate numerous DNA methylation data, making it inevitable to conduct cross-ONT-chemistry methylation analysis.
Raj Doshi, Evan Kinnear, Sujan Chatterjee, Prasun Guha, Qian Liu   +4 more
openaire   +3 more sources

From fluke to fragment: A multifaceted method for molecular sex identification and mitochondrial haplotyping from environmental DNA samples

Methods in Ecology and Evolution, EarlyView.
Abstract Environmental DNA (eDNA) analyses have become a powerful tool for non‐invasive biodiversity monitoring, yet the applicability of population‐genetic approaches to environmental samples remains largely unexplored. Even when genetic traces originate from a single individual, low target DNA concentrations and amplification or sequencing artefacts ...
Lauren Kelly Rodriguez, Sandra Schallhart, Philipp Hobmeier, Thomas G. Curran, Sergi Pérez‐Jorge, Rui Prieto, Cláudia Oliveira, Mónica A. Silva, Bettina Thalinger   +8 more
wiley   +1 more source