Results 101 to 110 of about 5,800,592 (273)

A comparison of short- and long-read whole-genome sequencing for microbial pathogen epidemiology

mSystems
Whole-genome sequencing provides the highest resolution for characterizing pathogen evolution, epidemiology, and diagnostics. Genome assemblies contain information on the identity and potential phenotypes of a pathogen.
Andrea M. Schiffer, Arafat Rahman, Wendy Sutton, Melodie L. Putnam, Alexandra J. Weisberg   +4 more
doaj   +1 more source

Is Oxford Nanopore Technology ready for clinical diagnostics?

, 2017
Bridging the “valley of death” between scientific and technological innovation and clinical implementation. Quality considerations for read mapping, variant calling and deletion mapping with Long Read Sequencing.
Taylor, Graham R., Kesia Brown, Bond, Andrew, Yau, Michael   +3 more
openaire   +1 more source

Resolving Complex Structural Variants in Undiagnosed Rare Movement Disorders via Multimodal Genomics and Multi‐omics

Movement Disorders, EarlyView.
Abstract Background Long‐read sequencing and multi‐omic analytical frameworks are increasingly being adopted in rare disease diagnostics. However, clinical workflows comprehensively integrating these methodologies remain uncommon. Objective This study aimed to assess the potential and limitations of integrating long‐read genomic, transcriptomic, and ...
Ugo Sorrentino, Melanie Brugger, Alice Saparov, Ivana Dzinovic, Philip Harrer, Sandy Lösecke, Kevork Derderian, Martin Pavlov, Robert Kopajtich, Holger Prokisch, Arcangela Iuso, Maria Mazurkiewicz‐Bełdzińska, Deike Weiss, Christina Ludwig, Miriam Abele, Julia Mergner, Juliane Winkelmann, Theresa Brunet, Michał Schinwelski, Elisabeth Graf, Katja Kloth‐Stachnau, Theresia Herget, Magdalena Krygier, Michael Zech   +23 more
wiley   +1 more source

The second Oxford Nanopore read ever published

, 2014
To our knowledge this is the second Oxford Nanopore read published. This read defines some completely uninteresting features including a 30S ribosomal subunit protein S1 and an integration host factor.
Audrey Farbos (579006), Paul O'Neill (513635), Konrad Paszkiewicz (577690), Karen Moore (579005)   +3 more
core   +1 more source

BulkVis: a graphical viewer for Oxford nanopore bulk FAST5 files

Bioinformatics, 2018
Abstract Motivation The Oxford Nanopore Technologies (ONT) MinION is used for sequencing a wide variety of sample types with diverse methods of sample extraction. Nanopore sequencers output FAST5 files containing signal data subsequently base called to FASTQ format.
Alexander Payne, Nadine Holmes, Vardhman K. Rakyan, Matthew Loose   +3 more
openaire   +3 more sources

Biosurfactant production by Pseudomonas aeruginosa isolated from mining waste: From genomic analysis to development of a polymeric composite for removal of hexavalent chromium

Polymer International, EarlyView.
Biosurfactant was produced by Pseudomonas aeruginosa using andiroba waste from Amazonia. A biosurfactant–alginate composite (Alg/BioS) was successfully synthesized via ionotropic gelation, and achieved up to 70% Cr6+ removal from water. Abstract Environmental contamination by heavy metals represents a significant challenge due to their persistence in ...
Paulo Diniz Austriaco, Emilly Cruz da Silva, Glenda Sores Gomes, Joane Almeida Alves, Amanda Vidal, Leandro Argolo, Rafaela Ribeiro, Luciana Souza, José Augusto Pires Bitencourt, Leonardo Brasil Felipe, Leonie Asfora Sarubbo, Sidnei Cerqueira dos Santos   +11 more
wiley   +1 more source

Leishmania infantum JPCM5 sequencing reads generated from total RNA by Oxford Nanopore technology

This project was aimed to sequence total RNA from Leishmania infantum JPCM5 promastigotes using the Oxford Nanopore Technology (ONT) methodology. This dataset consists of two Fastq files, generated by MinION Mk1C (ONT) MC-114562 device (Basecalling was ...
Solana, Jose Carlos, Sanchez-Salvador, Alejandro, Adán Jiménez, Javier, Requena Rolanía, Jose María   +3 more
core   +1 more source

The second ever Oxford Nanopore read published

, 2014
To our knowledge this is the second Oxford Nanopore read published. This read defines some completely uninteresting features including a 30S ribosomal subunit protein S1 and an integration host factor.
Audrey Farbos (579006), Paul O'Neill (513635), Konrad Paszkiewicz (577690), Karen Moore (579005)   +3 more
core   +1 more source

First genome sequence of a European Alternaria brassicae isolate and genes involved in early development of alternaria leaf spot on Brassica juncea

Pest Management Science, EarlyView.
This article reports the first genome sequence of a UK Alternaria brassicae isolate. Dual RNA‐sequencing profiling of A. brassicae‐infected Brassica juncea leaves identified differentially expressed genes involved in pathogenicity and host response pathways in moderately resistant Sej‐2 (2) and moderately susceptible Pusa Jaikisan cultivars.
Kevin M. King, Graham McLaughlin, Chinthani S. Karandeni Dewage, Zedi Gao, David J. Hughes, Jonathan S. West, Henrik U. Stotz   +6 more
wiley   +1 more source

Nanopore-only assemblies

, 2018
These files contain the best nanopore-only assembly produced by ABruijn (pre-corrected + Nanopolish), Canu (Racon x2 + Nanopolish), Flye (pre-corrected + Nanopolish), Miniasm + Minimap (filtered to 100x coverage with Filtlong, Racon x5 + Nanopolish) and ...
Natalie Ring (5116013)
core   +1 more source