Results 91 to 100 of about 5,800,592 (273)

Multi‐omics–driven precision medicine

iMeta, EarlyView.
Multi‐omics‐driven precision medicine (MODPM) provides a multiscale, continuously learnable framework that integrates genomics, epigenomics, transcriptomics, proteomics, metabolomics, microbiome profiles, and clinical data. Powered by artificial intelligence and foundation models, MODPM enables cross‐modal representation learning, contextual modeling ...
Huibo Li, Zhe Zhao, Yifan Zhang, Yao Ma, Gaofei Hu, Min Zeng, Zhanqun Yang, Zixuan Zhao, Xin Zhou, Wei Hu, Yuxuan Sun, Meng Su, Jun Li, Matthew Whiteman, Wei Fu, Chao Zhong, Lemin Zheng, Long Chen, Hairong Lv, Rongsheng Zhao, Yi Zhun Zhu   +20 more
wiley   +1 more source

De novo yeast genome assemblies from MinION, PacBio and MiSeq platforms

Scientific Reports, 2017
Long-read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base-pairs and maximum lengths reaching 100,000 base- pairs.
Francesca Giordano, Louise Aigrain, Michael A Quail, Paul Coupland, James K Bonfield, Robert M Davies, German Tischler, David K Jackson, Thomas M Keane, Jing Li, Jia-Xing Yue, Gianni Liti, Richard Durbin, Zemin Ning   +13 more
doaj   +1 more source

Deciphering transcriptome complexity via long‐read sequencing

iMeta, EarlyView.
Long‐read sequencing is transforming transcriptomics from gene‐level quantification to isoform‐resolved interpretation by directly resolving full‐length transcript structures, transcription in repetitive regions, and linked molecular features. This review provides an end‐to‐end roadmap covering sequencing platforms, library preparation strategies ...
Chuwen Xu, Jia Li, Chenxi Yin, Keying Li, Tian Wang, Shiwen Gao, Yue Yu, Cheng Chang, Ye Wang, Guoqing Tong, Liang Gong, Wen Hu, Guoliang Chai, Yilai Han, Qian Qin, Suoqin Jin, Xiufen Zou, Bo Li, Tianyuan Zhang, Ana Conesa, Yunhao Wang, Dingjie Wang   +21 more
wiley   +1 more source

Human genome sequencing on Oxford Nanopore PromethION

, 2018
For benchmarking, development and evaluation of tools we have sequenced the Yoruban reference genome NA19240 to 80x coverage using Oxford Nanopore PromethION. Additionally, we are developing a workflow for efficient detection and annotation of structural variation from long read sequencing data, integrating tools such as Sniffles and NanoSV.
Coster, Wouter De, Pooter, Tim De, Roeck, Arne De, D'Hert, Svenn, Dongen, Jasper Van, Rijk, Peter De, Strazisar, Mojca, Broeckhoven, Christine Van   +7 more
openaire   +1 more source

Gut Enterococcus faecium derived leucic acid mediates the antiobesity effect of dietary fiber

iMeta, EarlyView.
This study identified Enterococcus faecium as a gut bacterium with anti‐obesity activity, which alleviated adipocyte hypertrophy, dyslipidemia, and hepatic steatosis by converting leucine into leucic acid (LEA) to regulate mitochondrial oxidative phosphorylation.
Baifei Hu, Qianru Gao, Junmin Hui, Mengqiong Wang, Haiyan Gao, Kai Li, Junping Zheng, Zhe Dai, Songlin Liu, Hongtao Liu   +9 more
wiley   +1 more source

Evaluating nanopore sequencing data processing pipelines for structure variation identification, supplemental data

, 2019
nanopore_03262018.fastq.gz Description: DNA sample of individual NS12911 was sequenced by Oxford Nanopore (ONT) MinION using flowcell FLO-MIN106D. The library was constructed using ONT 1D Genomic DNA by ligation protocol.

core   +1 more source

Different gut microbiome dysbioses impact the evolutionary fate of hypervirulent Klebsiella pneumoniae

iMeta, EarlyView.
Transient exposure to ampicillin results in restructuring of microbiota to favor oxygen‐tolerant bacteria, whereas osmotic laxative perturbation preserves Bacteriodota. Ampicillin‐ but not laxative‐exposed environment favors establishment of rmpA phase variants that lost hypermucoviscosity but short‐chain fatty acids present in the laxative‐exposed gut
Carey Lim, Jialing Zheng, Guoxiang Cheam, Melvin Yong, Hanrong Chen, Andrew Ting, Yahua Chen, Qingqing Wang, Niranjan Nagarajan, Yunn‐Hwen Gan   +9 more
wiley   +1 more source

De novo Nanopore read quality improvement using deep learning

BMC Bioinformatics, 2019
Background Long read sequencing technologies such as Oxford Nanopore can greatly decrease the complexity of de novo genome assembly and large structural variation identification.
Nathan LaPierre, Rob Egan, Wei Wang, Zhong Wang   +3 more
doaj   +1 more source

Forensic DNA phenotyping using Oxford Nanopore Sequencing system

ELECTROPHORESIS
AbstractIn forensic science, the demand for precision, consistency, and cost‐effectiveness has driven the exploration of next‐generation sequencing technologies. This study investigates the potential of Oxford Nanopore Sequencing (ONT) Technology for analyzing the HIrisPlex‐S panel, a set of 41 single nucleotide polymorphism (SNP) markers used to ...
Veysel Sapan, Sumeyye Zulal Simsek, Gonul Filoğlu, Ozlem Bulbul   +3 more
openaire   +3 more sources

From Uncertainty to Pathogenicity: Resolving a CSF1R Variant of Uncertain Significance Using Long‐Read Transcriptomics

Movement Disorders, EarlyView.
Abstract Background CSF1R‐related disorder (CSF1R‐RD) is a severe autosomal dominant leukoencephalopathy characterized by progressive cognitive, neuropsychiatric, and motor decline. Although genetic testing is widely available, numerous likely pathogenic variants in CSF1R frequently remain classified as variants of uncertain significance (VUS ...
Charles Wade, Kylie Montgomery, Gabriela E. Jones, Peter Sellars, Akram A. Hosseini, Simon Paine, Henry Houlden, Mina Ryten, David S. Lynch   +8 more
wiley   +1 more source