Results 111 to 120 of about 41,121 (277)

The PacBio libraries preparation for the Scapharca broughtonii. v1 [PDF]

open access: yes, 2019
This protocol is used to outline the process of the PacBio libraries preparation for the Scapharca broughtonii.
openaire   +1 more source

The draft genome of Ditylenchus dipsaci

open access: yesJournal of Nematology, 2019
Ditylenchus dipsaci is a devastating pest to many crops worldwide. We present the first genome sequence for this species, produced with PacBio sequencing and assembled with CANU.
Mimee Benjamin   +5 more
doaj   +1 more source

Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies and their applications to transcriptome analysis [version 2; referees: 2 approved]

open access: yesF1000Research, 2017
Background: Given the demonstrated utility of Third Generation Sequencing [Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)] long reads in many studies, a comprehensive analysis and comparison of their data quality and applications is ...
Jason L Weirather   +7 more
doaj   +1 more source

Recovery of Metagenome-Assembled Genomes from a Human Fecal Sample with PacBio HiFi Sequencing - Supplementary Material

open access: yes, 2022
Characterization of the fecal metagenome from a healthy human donor with the PacBio HiFi sequencing ...
PLAZA ONATE, Florian   +2 more
core   +1 more source

Chromosome‐Level Genome Assembly of Strauchbufo raddei and Multi‐Omic Insights Into Drought Adaptation

open access: yesIntegrative Zoology, EarlyView.
ABSTRACT Understanding how amphibians adapt to terrestrial and arid environments is important for elucidating vertebrate terrestrialization. In this study, we present the first chromosome‐level, high‐quality genome assembly of Strauchbufo raddei, constructed using PacBio HiFi long‐read sequencing and Hi‐C scaffolding. The assembled genome is 4.47 Gb in
Fanrui Chen   +7 more
wiley   +1 more source

From fluke to fragment: A multifaceted method for molecular sex identification and mitochondrial haplotyping from environmental DNA samples

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Environmental DNA (eDNA) analyses have become a powerful tool for non‐invasive biodiversity monitoring, yet the applicability of population‐genetic approaches to environmental samples remains largely unexplored. Even when genetic traces originate from a single individual, low target DNA concentrations and amplification or sequencing artefacts ...
Lauren Kelly Rodriguez   +8 more
wiley   +1 more source

Chromosome-scale assembly comparison of the Korean Reference Genome KOREF from PromethION and PacBio with Hi-C mapping information [PDF]

open access: yes, 2019
Background: Long DNA reads produced by single-molecule and pore-based sequencers are more suitable for assembly and structural variation discovery than short-read DNA fragments.
Lee, Semin   +9 more
core   +3 more sources

EMITS: Expectation–maximization abundance estimation for fungal ITS communities from long‐read sequencing

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract As long‐read amplicon sequencing becomes routine for fungal metabarcoding, species‐level abundance estimation from ITS amplicons remains limited by naive best‐hit classification. Assigning each read to its single highest‐scoring reference misattributes reads among closely related species that share similar ITS sequences, and divides the reads ...
Aaron O'Brien   +4 more
wiley   +1 more source

Soil Metagenome PacBio V2 v2

open access: yes
This protocol describes the sample collection to sequence acquisition workflow for PacBio Revio long-read sequencing of a complex soil sample using SMRT Bell library prep kit 2.0 and Revio SMRT cells.
Robert S James   +4 more
openaire   +1 more source

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