Results 81 to 90 of about 41,121 (277)
Alignment of the full-length tprK amino acid sequences obtained by PacBio sequencing.
, 2023 Alignment of the full-length tprK amino acid sequences obtained by PacBio sequencing.
Diane G. Edmondson (9515284) +4 more
core +1 more source
Sorghum T2T pangenome highlights comprehensive genomic variation
iMetaOmics, EarlyView.Three newly assembled telomere‐to‐telomere (T2T) genomes representing wild, landrace, and transformable germplasm were integrated with seven publicly available T2T genomes to construct a graph‐based pangenome. Comparative analyses revealed extensive centromere divergence, including distinct CEN137 monomer and CEN274 dimer organization, and showed that ...
Lihua Wang +10 more
wiley +1 more source
This protocol describes the sample collection to sequence acquisition workflow for PacBio Revio long-read sequencing of a complex soil sample using SMRT Bell library prep kit 2.0 and Revio SMRT cells.
Robert S James +4 more
openaire +1 more source
Improve homology search sensitivity of PacBio data by correcting frameshifts [PDF]
Bioinformatics, 2016 Single-molecule, real-time sequencing developed by Pacific BioSciences produces longer reads than secondary generation sequencing technologies such as Illumina. However, PacBio data has high sequencing error rate and most of the errors are insertion or deletion errors, causing frameshifts and leading to marginal alignment scores and short alignments in
Nan Du 0003, Yanni Sun
openaire +3 more sources
Long-read next-generation sequencing for molecular diagnosis of pediatric endocrine disorders [PDF]
Annals of Pediatric Endocrinology & MetabolismRecent advances in long-read next-generation sequencing (NGS) have enabled researchers to identify several pathogenic variants overlooked by short-read NGS, array-based comparative genomic hybridization, and other conventional methods.
Yoko Kuroki +3 more
doaj +1 more source
Pest Management Science, EarlyView.Transcriptome sequencing of Ceutorhynchus obstrictus revealed RNA interference (RNAi) targets; double‐stranded RNA microinjection reduced transcript levels and increased mortality, with αCOP and Rop showing strongest lethal effects, indicating promising RNAi‐based pest control candidates.
Apurva Vikas Sabnis +8 more
wiley +1 more source
Genome-wide profiling of highly similar paralogous genes using HiFi sequencing
Nature CommunicationsVariant calling is hindered in segmental duplications by sequence homology. We developed Paraphase, a HiFi-based informatics method that resolves highly similar genes by phasing all haplotypes of paralogous genes together.
Xiao Chen +22 more
doaj +1 more source
Phytotherapy Research, EarlyView.ABSTRACT Alzheimer's disease (AD) is a progressive neurodegenerative disorder associated with cognitive decline and neuroinflammation. Xanthoceras sorbifolia Bunge oil (XSBO), a woody plant oil rich in bioactive fatty acids, has shown potential health benefits.
Lijing Du +8 more
wiley +1 more source
Insect Science, EarlyView.The mitogenome was transcribed into complete primary polycistrons on both strands. The heteropteran mitochondrial transcription termination factor (HmTTF) functions as a bidirectional attenuator rather than a terminator, and co‐evolution of HmTTF and the binding sites was observed.
Shiwen Xu +6 more
wiley +1 more source
Plant Methods, 2020 Background The number of studies using third-generation sequencing utilising Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas.
Jiawen Cui +5 more
doaj +1 more source