Results 11 to 20 of about 183,075 (317)
Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices
STAR Protocols, 2021 Summary: Patch-clamp and multi-electrode array electrophysiology techniques are used to measure dynamic functional properties of neurons. Whole-cell and cell-attached patch-clamp recordings in brain slices can be performed in voltage-clamp and current ...
Kevin M. Manz +3 more
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In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives
Sensors, 2021 Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp ...
Asako Noguchi +2 more
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Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience
Frontiers in Neural Circuits, 2015 Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal ...
Can eTao +3 more
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A comparison of the performance and application differences between manual and automated patch-clamp techniques. [PDF]
Curr Chem Genomics, 2012 The patch clamp technique is commonly used in electrophysiological experiments and offers direct insight into ion channel properties through the characterization of ion channel activity.
Yajuan X, Xin L, Zhiyuan L.
europepmc +2 more sources
Pflugers Archiv European Journal of Physiology, 1989 (1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in situ. (2) A vibrating tissue slicer was used to cut thin slices in which individual neurones could be identified visually.
Arthur Konnerth +2 more
exaly +5 more sources
Automatic deep learning-driven label-free image-guided patch clamp system [PDF]
Nature Communications, 2021 Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. Here, we demonstrate a tool that fully automatically performs electrophysiological recordings in label-free tissue slices. The automation covers the detection of cells in
K. Koos +13 more
semanticscholar +2 more sources
Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function
Frontiers in Pharmacology, 2017 The chloride (Cl-) channel cystic fibrosis transmembrane conductance regulator (CFTR) is defective in cystic fibrosis (CF), and mutation of its encoding gene leads to various defects such as retention of the misfolded protein in the endoplasmic reticulum,
A. Billet +3 more
semanticscholar +2 more sources
Cellular and Molecular Bioengineering, 2011 Patch clamp is a powerful tool for studying the properties of ion-channels and cellular membrane. In recent years, planar patch clamp chips have been fabricated from various materials including glass, quartz, silicon, silicon nitride, polydimethyl ...
Joseph E Candiello +2 more
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Biochimica Et Biophysica Acta - Biomembranes, 2004 Anna De Simoni, Alessandro Formenti
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Patch clamp techniques used for studying synaptic transmission in slices of mammalian brain.
Quarterly Journal of Experimental Physiology, 1989 Procedures are described for recording postsynaptic currents from neurones in slices of rat brain using patch clamp techniques. The method involves cutting brain slices (120-300, µm thick) with a vibrating microtome followed by localization of cell ...
Bert Sakmann +3 more
semanticscholar +3 more sources