Results 21 to 30 of about 183,075 (317)
Patch-clamp fluorometry: electrophysiology meets fluorescence.
Biophysical Journal, 2014 Ion channels and transporters are membrane proteins whose functions are driven by conformational changes. Classical biophysical techniques provide insight into either the structure or the function of these proteins, but a full understanding of their ...
J. Kusch, G. Zifarelli
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Frontiers in Aging Neuroscience, 2021 A patch-clamp recording in slices generated from the brain or the spinal cord has facilitated the exploration of neuronal circuits and the molecular mechanisms underlying neurological disorders.
Yang Li +5 more
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Whole-cell and Perforated Patch-clamp Recordings from Acutely-isolated Murine Sino-atrial Node Cells
Bio-Protocol, 2020 Cardiac pacemaker cells of the sino-atrial node are responsible for the initiation of the heart beat and express an array of ion channels. The patch-clamp technique is the gold standard method for investigating the function of ion channels expressed in ...
Qadeer Aziz +2 more
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Nanotechnology: new opportunities for the development of patch‐clamps
Journal of Nanobiotechnology, 2021 The patch-clamp technique is one of the best approaches to investigate neural excitability. Impressive improvements towards the automation of the patch-clamp technique have been made, but obvious limitations and hurdles still exist, such as ...
Jia Gao +4 more
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Equilibrium Research, 2008 The patch clamp technique enables the analyses of various ion channel characteristics of individual cells in vitro. The most important difference from previous techniques is that the microelectrode (pipette) is firmly attached to the cell surface instead of being inserted into the cells, and the so-called "giga-ohm seal" is attained. Usually, the whole
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Frontiers in Pharmacology, 2021 The transient receptor potential vanilloid type 1 (TRPV1) channel is a non-selective cation channel expressed with transient receptor potential ankyrin type 1 (TRPA1) in small and medial size neurons of the dorsal root ganglions and trigeminal ganglions.
Kaori Takahashi +6 more
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Stem Cell Research, 2023 Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques.
Rajesh Vivekanandan +8 more
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Alpha-Adrenoceptor Antagonists Improve Memory by Activating -methyl-D-Aspartate-Induced Ion Currents in the Rat Hippocampus [PDF]
International Neurourology Journal, 2015 Purpose: Alpha1 (α1)-adrenoceptor antagonists are widely used to treat lower urinary tract symptoms. These drugs not only act on peripheral tissues, but also cross the blood-brain barrier and affect the central nervous system.
Chang Hee Kim +9 more
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The patch-clamp technique in the study of secretion [PDF]
Trends in Neurosciences, 1989 One of the basic cellular functions of virtually every cell type is the exocytotic release of molecules synthesized, stored and packaged into intracellular vesicles or granules. Over decades much effort has been concentrated on elucidating the chain of events leading to exocytosis.
Penner, R., Neher, E.
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