Results 181 to 190 of about 11,551 (206)
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Allelic Variation in the Porcine MYF5 Gene Detected by PCR–SSCP
Molecular Biotechnology, 2008The MYF5 gene has been reported to be integral to muscle growth and development, and hence it has been considered as a candidate gene for meat selection programs in pig. To ascertain whether there was variation in the porcine MYF5 gene, we have developed a method of PCR-single-strand conformational polymorphism (PCR-SSCP) analysis.
Sajee, Kunhareang +2 more
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[AP-PCR and AP-PCR-SSCP analyses].
Nihon rinsho. Japanese journal of clinical medicine, 1996DNA fingerprinting by arbitrarily primed polymerase chain reaction (AP-PCR) is a simple and informative method to investigate genetic changes in cancers. The discovery of a mutator phenotype in hereditary non-polypotic colon cancer by the AP-PCR fingerprinting demonstrated the usefulness of this unbiased method.
J, Yasuda, A, Okano, T, Sekiya
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Distribution of MN genotypes detected by PCR-SSCP analysis
International Congress Series, 2003Abstract Genotyping of the MN blood system was performed by means of PCR-single strand conformation polymorphism (PCR-SSCP) analysis. Twelve band patterns corresponding to each MN genotype composed of alleles MG, MT, N1, N2 and NV were detected. In general, MG or N1>MT>N2 in order of decreasing frequency was observed as four common alleles in five ...
N. Nakayashiki +6 more
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Polymorphism in the human E-selectin gene detected by PCR-SSCP
Human Genetics, 1994By using the non-isotopic single-strand conformation polymorphism (SSCP) technique to analyse products of the polymerase chain reaction (PCR), we detected a 561-adenine to cytosine substitution resulting in an amino acid exchange from serine to arginine at position 128 of the E-selectin gene.
K, Wenzel, R, Hanke, A, Speer
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No TP53 mutations in neuroblastomas detected by PCR‐SSCP analysis
Genes, Chromosomes and Cancer, 1994AbstractWe have analysed 29 neuroblastomas for TP53 mutations in exons 5 to 8 by means of the polymerase chain reaction in combination with the single‐strand conformation polymorphism technique. We could not detect any mutation. These results indicate that, in contrast to the majority of tumors so far studied, TP53 mutations do not seem to be important
J S, Castresana +6 more
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The application of asymmetric PCR-SSCP in gene mutation detecting
Frontiers of Agriculture in China, 2008The advantages and disadvantages of asymmetric PCR-SSCP and the traditional PCR-SSCP were compared in this study. The mutations in 3′UTR of Smad4 gene of Luxi cattle and the Holstein cow were analyzed by asymmetric PCR-SSCP and one insert “T” mutation and one G/A mutation in this region were found.
Xiaohui Zhang +5 more
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Efficient detection of alport syndromeCOL4a5 mutations with multiplex genomic PCR-SSCP
American Journal of Medical Genetics, 2001We have performed effective mutation screening of COL4A5 with a new method of direct, multiplex genomic amplification that employs a single buffer condition and PCR profile. Application of the method to a consecutive series of 46 United States patients with diverse indications of Alport syndrome resulted in detection of mutations in 31 cases and of ...
D F, Barker +3 more
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[HLA-DQB2 genotypes analyzed by PCR/SSCP method].
Nihon hoigaku zasshi = The Japanese journal of legal medicine, 1996When the PCR products amplified by the primers prepared at the 11th HLA Workshop (DQBAMP-A, DQBAMP-B) were analyzed directly by the SSCP method, one or two pairs of characteristic bands were detected other than those attributed to DQB1, and a total of three kind of paired bands were detected.
K, Akiyama +3 more
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Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols
2003Screening for mutations prior to sequencing can reduce the time and costs of identifying mutations. When the DNA sequence is known, the technique of detecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (1).
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DLA‐DRB1 and DLA‐DQB1 histocompatibility typing by PCR‐SSCP and sequencing
Tissue Antigens, 1998Abstract: The dog has been an important model for solid organ and hematopoietic stem cell transplantation for over 30 years. Fundamental to the continuing usage of the model is the development of molecular‐based histocompatibility typing of donors and recipients.
J L, Wagner, J D, Works, R, Storb
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