Results 31 to 40 of about 6,961 (161)

Origins of T‐cell‐mediated autoimmunity in acquired aplastic anaemia

British Journal of Haematology, Volume 206, Issue 4, Page 1035-1053, April 2025.
Acquired aplastic anaemia (AA) is an autoimmune bone marrow failure disease caused by a cytotoxic T‐cell–mediated attack on haematopoietic stem and progenitor cells (HSPCs). Despite significant progress in understanding T‐cell repertoire alterations in AA, the identification of specific pathogenic T cells remains elusive.
Aura Enache, Shannon A. Carty, Daria V. Babushok   +2 more
wiley   +1 more source

The Association Between IL‐8 Gene Polymorphisms and the Risk of Several Types of Cancer, Especially in Gastric Cancer

Cancer Reports, Volume 8, Issue 1, January 2025.
ABSTRACT Background Changes in functional genetic polymorphisms may increase or decrease the risk of cancer in patients. Nowadays, the association between polymorphisms in the interleukin‐8 (IL‐8) gene and the susceptibility of cancer risk have been investigated in many studies, however, above relationships remain unclear. Aim The current study aims to
Bin Xu, Yidan Yan
wiley   +1 more source

Software for Machine-Independent Quantitative Interpretation of SSCP in Capillary Array Electrophoresis (QUISCA)

BioTechniques, 2002
PCR single-stranded conformational polymorphism (SSCP) analysis is a simple and rapid electrophoretic technique for the sensitive detection of sequence variants of PCR products.
Koichiro Higasa, Yoji Kukita, Shingo Baba, Kenshi Hayashi   +3 more
doaj   +1 more source

An efficient CRISPR‐Cas12a‐mediated MicroRNA knockout strategy in plants

Plant Biotechnology Journal, Volume 23, Issue 1, Page 128-140, January 2025.
Summary In recent years, the CRISPR‐Cas9 nuclease has been used to knock out MicroRNA (miRNA) genes in plants, greatly promoting the study of miRNA function. However, due to its propensity for generating small insertions and deletions, Cas9 is not well‐suited for achieving a complete knockout of miRNA genes.
Xuelian Zheng, Xu Tang, Yuechao Wu, Xiaoqin Zheng, Jianping Zhou, Qinqin Han, Yalan Tang, Xinxuan Fu, Jiao Deng, Yibo Wang, Danning Wang, Shuting Zhang, Tao Zhang, Yiping Qi, Yong Zhang   +14 more
wiley   +1 more source

Screening for mutations in human alpha-globin genes by nonradioactive single-strand conformation polymorphism

Brazilian Journal of Medical and Biological Research, 2003
Point mutations and small insertions or deletions in the human alpha-globin genes may produce alpha-chain structural variants and alpha-thalassemia.
S.B. Jorge, M.B. Melo, F.F. Costa, M.F. Sonati   +3 more
doaj   +1 more source

Polymorphism in the Growth Hormone Gene and Its Association With Growth‐Related Traits in Common Carp, Cyprinus carpio (Linnaeus, 1778), in the Inland Saline Production System

Journal of Applied Ichthyology, Volume 2025, Issue 1, 2025.
Identification of polymorphism in growth‐related genes and their association with growth traits is an area of intense research. The growth rate is the most sought‐after economic trait in the breeding program of fish. The significant association of polymorphism will aid in marker‐assisted selection and speed up the genetic improvement program.
Anjali Kumari, Mujahidkhan A. Pathan, Raghul R., Nagaraja P. S., Susitharan V., Priyanka Sanwal, Angom Lenin Singh, Mohd Ashraf Rather   +7 more
wiley   +1 more source

[PGM1 genotyping by PCR-SSCP].

Fa yi xue za zhi, 2004
PGM1 genotyping by PCR-SSCP analysis.Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318.
Hai-yan, Song, Qing-en, Yang, Chun-ying, Yu   +2 more
openaire   +1 more source

Evaluating the Impact of Laundry Detergents on the Skin Microbiome of Atopic Dermatitis Patients—A Clinical Study

Health Science Reports, Volume 7, Issue 12, December 2024.
ABSTRACT Background and Aims Remnants of laundry detergent ingredients are suspected to be in constant contact with the skin. Allergy sufferers need assurance that the chemicals in everyday products do not cause such disruption. The purpose of this study was to evaluate the effects and tolerability of common laundry detergents on the skin microbiome in
Demetrios Christou, Katarina Stevanovic, Stefan Evers, Mirko Weide, Torsten Zuberbier   +4 more
wiley   +1 more source

Integrating visual and molecular approaches for fish eggs analysis: A study on formaldehyde fixation and storage procedures

Limnology and Oceanography: Methods, Volume 22, Issue 11, Page 853-861, November 2024.
Abstract Accurate taxonomic classification and developmental stage determination of fish eggs are crucial for ecological monitoring, conservation efforts, and stock assessments. Traditional methods for fish and fisheries rely on visual examination of morphological traits, but they face challenges due to species overlap especially for early stages ...
Bahar Mozfar, Anders Thorsen, Alejandro Mateos‐Rivera, Geir Dahle, Rasmus Skern‐Mauritzen, Henning Wehde, Bjørn A. Krafft   +6 more
wiley   +1 more source

Haplotype association of ovine leptin gene on breeding value of body measurements in Makooei sheep breed [PDF]

Biotechnology in Animal Husbandry, 2014
The research was undertaken to find association of genetic variation in the exon 3 of the leptin gene and breeding value of body weight traits in Makooei sheep breed using single strand conformation polymorphism (SSCP).
Sadeghi S., Hajihosseinlo A., Bohlouli M.   +2 more
doaj