Results 41 to 50 of about 6,961 (161)

PCR-SSCP of the 16S rRNA gene, a simple methodology for species identification of fish eggs and larvae

Scientia Marina, 2006
Patterns of the 16S rRNA gene obtained in 8 and 12% acrylamide gels by the SSCP (Single Strand Conformation Polymorphism) method were different for various marine fish species (Macrorhamphosus scolopax, Scomber scombrus, Lepidorhombus boscii, L ...
Eva Garcia-Vazquez, Paula Alvarez, Placida Lopes, Nikoletta Karaiskou, Juliana Perez, Ana Teia, José L. Martinez, Laurentina Gomes, Costas Triantaphyllidis   +8 more
doaj   +1 more source

Identification of meat adulteration in minced meat samples labeled as beef and mutton in Tehran stores using duplex PCR

Food Science &Nutrition, Volume 12, Issue 10, Page 7504-7511, October 2024.
In this study, we conducted a comprehensive analysis of 300 minced meat samples labeled as beef and mutton, collected randomly from Tehran stores over a span of 4 years (2018–2022). Employing duplex polymerase chaiin reaction (PCR) techniques, we meticulously examined the meat samples, aiming to compare their actual contents with the information ...
Mohammad Doroudian, Mahdieh Soezi, Milad Rasouli, Maryam Arshadi Far, Maryam Yousefi Dehbidi, Pedram Maafi, Forough Yousefi, Mohammad‐Reza Ajouri, Bijan Omidi   +8 more
wiley   +1 more source

Medical semiology of patients with monogenic obesity: A systematic review

Obesity Reviews, Volume 25, Issue 10, October 2024.
Summary Patients with monogenic obesity display numerous medical features on top of hyperphagic obesity, but no study to date has provided an exhaustive description of their semiology. Two reviewers independently conducted a systematic review of MEDLINE, Embase, and Web of Science Core Collection databases from inception to January 2022 to identify ...
Emeline Renard, Ariane Thevenard‐Berger, David Meyre   +2 more
wiley   +1 more source

Chromosomal inversions harbour excess mutational load in the coral, Acropora kenti, on the Great Barrier Reef

Molecular Ecology, Volume 33, Issue 16, August 2024.
Abstract The future survival of coral reefs in the Anthropocene depends on the capacity of corals to adapt as oceans warm and extreme weather events become more frequent. Targeted interventions designed to assist evolutionary processes in corals require a comprehensive understanding of the distribution and structure of standing variation, however ...
Jia Zhang, Nadja M. Schneller, Matt A. Field, Cheong Xin Chan, David J. Miller, Jan M. Strugnell, Cynthia Riginos, Line Bay, Ira Cooke   +8 more
wiley   +1 more source

Detection of allelic variants of kappa-caseinin Harton del Valle cattle Detección de variantes alélicas de la kappa-caseína en bovinos Hartón del Valle

Acta Agronómica, 2007
DNA from 2 males and 34 females of Hartón del Valle bovine breed, to detect polymorphisms by PCR-RFLP (polymerase chain reaction – restriction fragments length polymorphism) and PCR-SSCP (polymerase chain reaction– single strand conformation ...
Cárdenas Heiber, Muñoz Florez Jaime Eduardo, Posso Terranova Andrés Mauricio, Naranjo Julián   +3 more
doaj  

Multiple fluorescence-based PCR-SSCP analysis.

BioTechniques, 1994
Multiple fluorescence-based polymerase chain reaction single-strand conformation polymorphism (MF-PCR-SSCP) was developed. The target sequence was amplified by PCR using forward and reverse primers labeled with two different fluorescent dyes at their 5' ends.
H, Iwahana, K, Yoshimoto, N, Mizusawa, E, Kudo, M, Itakura   +4 more
openaire   +1 more source

Twenty‐three years of PCR‐based seafood authentication assay development: What have we learned?

Comprehensive Reviews in Food Science and Food Safety, Volume 23, Issue 4, July 2024.
Abstract Seafood is a prime target for fraudulent activities due to the complexity of its supply chain, high demand, and difficult discrimination among species once morphological characteristics are removed. Instances of seafood fraud are expected to increase due to growing demand.
Maleeka Singh, Robert G. Young, Rosalee S. Hellberg, Robert H. Hanner, Maria G. Corradini, Jeffrey M. Farber   +5 more
wiley   +1 more source

Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

International Journal of Bio-Resource and Stress Management, 2021
Heat shock proteins (HSPs) are a type of molecular chaperones that aid in the recovery of stressed cells and serve as a major system for intracellular self-defense.
J. Sai Prasanna, S. T. Viroji Rao, M. Gnana Prakash, Suresh Rathod, P. Kalyani, B. Rajith Reddy   +5 more
doaj  

PCR-SSCP Analysis and DNA Sequencing as an Important Method for Detecting Mutation of INGI gene in Head and Neck Squamous Cell Carcinomas

Journal of Dentistry Indonesia, 2015
Tumor specific mutation of the candidate tumor suppressor gene INGI ws demonstrated using PCR-SSCP and DNA sequencing analysis. The mutant sample showed an amino acid change from aspangine (AAC) to serine (AGC) at codon 214 in the INGI gene from the DNA ...
Elza Ibrahim Auerkari, Mehmet Gunduz, Mamoru Ouchida   +2 more
doaj   +1 more source

Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA

Molecular and Cellular Probes, 1997
Fourteen species of parasitic nematodes (order Strongylida) were characterized using a polymerase chain reaction-linked single strand conformation polymorphism technique (PCR-SSCP). The rDNA region spanning the second internal transcribed spacer (ITS-2) was amplified from parasite DNA by PCR.
R B, Gasser, J R, Monti
openaire   +2 more sources