Results 61 to 70 of about 6,961 (161)

HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP

open access: yesBrazilian Journal of Medical and Biological Research, 2001
In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR.
M.C. Abba, M.A. Gómez, C.D. Golijow
doaj   +1 more source

Genetic Variation of MSP-1 Gene in Plasmodium vivax Isolated from Patients in Hormozgan Province, Iran using SSCP-PCR

open access: yesIranian Journal of Parasitology, 2012
Background: The main goal of present study was to detect polymorphism in MSP-1 gene which is a major blood stage candidate for vaccine in Plasmodium vivax by Single Strand Conformational Polymor­phism-Polymerase Chain Reaction (SSCP-PCR).Methods: During ...
M Rezaeian   +5 more
doaj  

Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)

open access: yesGenetics and Molecular Biology, 2006
The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect a-lactalbumin polymorphism in a sample of 50 goats.
Dinesh Kumar   +5 more
doaj   +1 more source

Genetic Variation of MSP-1 Gene in Plasmodium vivax Isolated from Patients in Hormozgan Province, Iran using SSCP-PCR

open access: yesIranian Journal of Parasitology, 2015
Background: The main goal of present study was to detect polymorphism in MSP-1 gene which is a major blood stage candidate for vaccine in Plasmodium vivax by Single Strand Conformational Poly- morphism-Polymerase Chain Reaction (SSCP-PCR).
A. Miahipour   +5 more
doaj  

Comparacao das tecnicas de SSCP, DS-PCR, PCR-RFLP para detecção de mutação no gene mitocondrial 16S RRNA em populacoes de Melipona rufiventris

open access: yesBioscience Journal, 2006
Esse trabalho analisou a região 16S do DNA mitocondrial em populações de Melipona rufiventris do Espírito Santo, comparando a eficiência das técnicas moleculares SSCP (Single Strand Conformation Polymorfism), PCR-RFLP (Restriction Fragment Length ...
Cristina Soares de Sousa   +8 more
doaj  

PCR-SSCP analysis of GH gene in Sarda goats: a high variability and its preliminary effects on dairy performances

open access: yesItalian Journal of Animal Science, 2010
The growth hormone (GH) gene can be utilized as a major gene because in various domestic livestock its polymorphisms have been associated to milk traits.
Giuseppe Massimo Vacca   +4 more
doaj   +1 more source

PCR-SSCP检测急性髓系白血病患者FLT3基因及FLT3/ITD基因突变

open access: yesZhongguo shiyan zhenduanxue, 2009
目的研究急性髓系白血病(AML)患者DNA水平FLT3基因及其内部串联重复(ITD)突变。方法采用多聚酶链反应(PCR)联合单链构象多态性(SSCP)方法检测43例不同免疫分型AML患者DNA水平FLT3基因及FLT3/ITD基因突变。结果43例AML患者经PCR扩增,琼脂糖凝胶电泳FLT3基因检测全部阳性,未发现FLT3/ITD基因突变;SSCP电泳FLT3基因检测全部阳性,43例AML患者中有15例(35%)出现FLT3/ITD基因突变。结论PCR-SSCP适用于检测急性髓系白血病FLT3 ...
黄晶   +4 more
doaj  

Serotyping Dichelobacter nodosus with PCR-SSCP [PDF]

open access: yesJournal of Animal and Veterinary Advances, 2011
Tulay Ifakat Cagatay, Jon Hickford
openaire   +1 more source

结核分支杆菌耐异烟肼分子机制的研究

open access: yesZhongguo shiyan zhenduanxue, 2007
目的探讨耐异烟肼结核分支杆菌KatG基因突变在结核分支杆菌耐异烟肼耐药性测定中的应用价值。方法采用聚合酶链反应——单链构象多态性(PCR-SSCP)分析72株结核分支杆菌KatG基因突变。其中32株为异烟肼(INH)敏感株,40株为INH耐药株,用PCR-SSCP图谱鉴定扩增产物有无突变,H37RV标准株作对照。结果所有INH敏感株SSCP带谱与对照相同;40株INH耐药株中15株与对照相同,25株有不同程度的差异,INH耐药KatG基因突变或缺失的阳性率为62.5 ...
宋阳, 于宏波, 陈晶洁, 韩忠波
doaj  

Identificación por PCR-SSCP de genes de cefotaximasas en aislamientos hospitalarios de Enterobacteriaceae

open access: yesRevista Colombiana de Biotecnología, 2009
Las cefotaximasas (CTX-M) son las beta-lactamasas de espectro extendido más ampliamente diseminadas entre especies de la familia Enterobacteriaceae, y son la causa principal de resistencia en aislamientos causantes de infección intrahospitalaria.
José Ramón Mantilla Anaya   +3 more
doaj   +2 more sources

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