Results 131 to 140 of about 10,214 (167)
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Journal of Agricultural and Food Chemistry, 2010
In the quest of obtaining a molecular probe for in situ detection of pectin methylesterase (PME), the PME inhibitor (PMEI) was biotinylated and the biotinylated PMEI (bPMEI) was extensively characterized. Reaction conditions for single labeling of the purified PMEI with retention of its inhibitory capacity were identified.
Ruben P, Jolie +5 more
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In the quest of obtaining a molecular probe for in situ detection of pectin methylesterase (PME), the PME inhibitor (PMEI) was biotinylated and the biotinylated PMEI (bPMEI) was extensively characterized. Reaction conditions for single labeling of the purified PMEI with retention of its inhibitory capacity were identified.
Ruben P, Jolie +5 more
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Pectin lyase and pectin methylesterase production by Penicillium citrinum
Transactions of the British Mycological Society, 1979Penicillium citrinum Thorn produced enzymes with pectin lyase and pectin methylesterase activity when grown in liquid culture media containing pectic substances as sole carbon sources. The enzymes were also present in infected cocoa bean tissues. Slight pectin methylesterase activity was detected in healthy beans but a higher activity was present in ...
P.O. Olutiola, O.A. Akintunde
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Isolation and Characterization of Pectin Methylesterase from Papaya
Archives of Biochemistry and Biophysics, 1993Pectin methylesterase (PME) (EC 3.1.1.11) has been purified to apparent homogeneity from ripe papaya fruits. The purification protocol consisted of ammonium sulphate precipitation (60-80%) and cation exchange chromatography in CM Sepharose CL-6B and Mono S.
Lim, Y.-M., Chung, M.C.M.
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The presence of pectin methylesterase in cacao pulp
Experientia, 1973Die Pektin-Methylesterase wurde aus Kakaofruchtfleisch (Amazon) isoliert und teilweise gereinigt, wobei die Fermentaktivitat sich mit zunehmender Reife der Kakaobohne erhohte.
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THERMORESISTANCE OF PECTIN METHYLESTERASE IN SANGUINELLO ORANGE JUICE
Journal of Food Biochemistry, 2001The behavior of pectin methylesterase (EC 3.1.1.11) activity as a function of temperature was investigated in Sanguinello orange juice. The thermal inactivation of this enzyme was evaluated in the range of 75–95C and was found to be nonlinear. Due to very low activity content, after the thermal treatment at temperatures above 80C, the active enzyme was
DE SIO F +4 more
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Journal of Food Biochemistry, 2020
Pectin methylesterase (PME) extracted from muskmelon was purified by anion exchange chromatography. The specific activity of purified enzyme was 152.01 U/mg and relative molecular weight was ~69,000 Da. Methylesterase was characterized for various physicochemical factors to designate its suitability in the food industry applications.
Hosamani Prabhudev, A. H. Sneharani
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Pectin methylesterase (PME) extracted from muskmelon was purified by anion exchange chromatography. The specific activity of purified enzyme was 152.01 U/mg and relative molecular weight was ~69,000 Da. Methylesterase was characterized for various physicochemical factors to designate its suitability in the food industry applications.
Hosamani Prabhudev, A. H. Sneharani
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Separation of pectin methylesterases and polygalacturonases on monolithic columns
Journal of Chromatography B, 2007The most abundant isoforms of tomato pectin methylesterase (PME; EC 3.1.1.11; M(r) 26 kDa), polygalacturonase (PG; EC 3.2.1.15; PG1 with M(r) 82 kDa) and a basic protein with M(r) 42 kDa and unknown function were isolated from fresh tomato fruit by a fast chromatographic procedure on a Convective Interaction Media (CIM) short monolithic disk column ...
Irena, Vovk, Breda, Simonovska
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Pectin methylesterase as a factor of plant transcriptome stability
Molecular Biology, 2008Pectin methylesterase (PME), cell wall enzyme, is known as a factor of plant growth and morphogenesis. Recently, PME participation in gene silencing, plant virus reproduction and transgenesis was revealed. Here, the role of PME in antivirus resistance was studied.
T V, Gasanova +4 more
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New insights into pectin methylesterase structure and function
Trends in Plant Science, 2007In bacteria, fungi and plants, pectin methylesterases are ubiquitous enzymes that modify the degree of methylesterification of pectins, which are major components of plant cell walls. Such changes in pectin structure are associated with changes in cellular adhesion, plasticity, pH and ionic contents of the cell wall and influence plant development and ...
Jérôme, Pelloux +2 more
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Functional characterization of the gels prepared with pectin methylesterase (PME)-treated pectins
International Journal of Biological Macromolecules, 2009High- and low-methoxyl pectins were treated with pectin methylesterase (PME) and the functional properties of the resulting pectin gels were characterized. The degree of esterification of high- and low-methoxyl pectins decreased from 74.5% to 6.3% and 40.0% to 6.5%, respectively while not changing their molecular weight.
Young-Hee, Yoo +5 more
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