Results 41 to 50 of about 635,610 (300)

Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene [PDF]

, 2007
We aim to introduce the penicillin biosynthetic pathway into the methylotrophic yeast Hansenula polymorpha. To allow simultaneous expression of the multiple genes of the penicillin biosynthetic pathway, additional markers were required.
Gidijala, Loknath,, Kiel, Jan A.K.W.,, Klei, Ida J. van der,, Veenhuis, Marten,   +3 more
core   +2 more sources

Affinity of cefoperazone for penicillin-binding proteins [PDF]

Antimicrobial Agents and Chemotherapy, 1980
Cefoperazone (T-1551, CFP) a new semisynthetic cephalosporin, has a broad spectrum of antibacterial activity. We investigated the affinity of CFP to penicillin-binding proteins (PBPs) and the inhibition of peptidoglycan synthesis by CFP. CFP had high affinities for Escherichia coli PBP-3, -1Bs, -2, and -1A, in descending order, and low affinities for ...
N, Matsubara, S, Minami, M, Matsuhashi, M, Takaoka, S, Mitsuhashi   +4 more
openaire   +2 more sources

Allosteric p97 inhibitors can overcome resistance to ATP-competitive p97 inhibitors for potential anti-cancer therapy [PDF]

, 2020
A major challenge of targeted cancer therapy is the selection for drug‐resistant mutations in tumor cells leading to loss of treatment effectiveness. p97/VCP is a central regulator of protein homeostasis and a promising anti‐cancer target because of its ...
Chou, Tsui-Fen, Flint, Andrew J., Gan, Taiping, Li, Shan, Stott, Gordon M., Wang, Feng   +5 more
core   +1 more source

Penicillin-binding proteins in Borrelia burgdorferi [PDF]

Journal of Bacteriology, 1990
Penicillin-binding proteins were identified in Borrelia burgdorferi membranes. A 94-kilodalton penicillin-binding protein was the first to be labeled with tritiated penicillin and was the first band to disappear in a competition experiment. Its binding ability was destroyed when membranes were preboiled. In addition, several of these penicillin-binding
C, Urban, J J, Rahal, R J, Dattwyller, P, Gorevic, B J, Luft   +4 more
openaire   +2 more sources

Antimicrobial and molecular interaction studies on derivatives of curcumin against Streptococcus pneumoniae which caused pneumonia

Electronic Journal of Biotechnology, 2016
Background: The antimicrobial properties and molecular interaction analysis of curcumin and its derivatives against three different strains of Streptococcus pneumoniae (Penicillin-susceptible, Penicillin-intermediate and Penicillin-resistant) are studied.
Liang-Mei Li, Jun Li, Xiu-Ying Zhang
doaj   +1 more source

Specificity determinants for lysine incorporation in staphylococcus aureus peptidoglycan as revealed by the structure of a MurE enzyme ternary complex [PDF]

, 2013
Background: MurE controls stereo chemical incorporation of Lysine or diaminopimelate into peptidoglycan stem peptides Results: The structure of S.aureus MurE reveals an unexpected lack of specificity for Lysine within the active site.
Barreteau, Basavannacharya, Basavannacharya, Battye, Bertrand, Bertrand, Boniface, Boniface, Bouhss, Brünger, Bugg, Bugg, Chen, Clancy, Clarke, Clarke, Collaborative Computational Project, Number 4, Cox, Cressina, Crouvoisier, Cruickshank, De Lencastre, DeLano, Emsley, Ernst, Ernst, Favini-Stabile, Figueiredo, Gardete, Girardin, Gordon, Gorrec, Huber, Hutton, Jones, Kabsch, Lee, Liebeke, Lloyd, Lloyd, Lundqvist, Lysenko, Matteï, McCoy, Mei, Mengin-Lecreulx, Mengin-Lecreulx, Mohammadi, Murshudov, Münch, Ornelas-Soares, Painter, Paradis-Bleau, Paradis-Bleau, Patin, Perdih, Ray, Read, Royet, Sauvage, Scheffers, Schneider, Schouten, Smith, Sosič, Sova, Thedieck, Vagin, Vollmer, Vollmer, Walker, Young, Zawadzke   +72 more
core   +2 more sources

Genome-wide identification of genes required for alternative peptidoglycan cross-linking in Escherichia coli revealed unexpected impacts of β-lactams

Nature Communications, 2022
β-lactam-induced bacterial killing is complex and not fully resolved. Authors carry out a genome-wide analysis, through penicillin-binding protein replacement, to identify genes essential for drug efficacy.
Henri Voedts, Sean P. Kennedy, Guennadi Sezonov, Michel Arthur, Jean-Emmanuel Hugonnet   +4 more
doaj   +1 more source

Emergence of Meropenem Resistance Among Cefotaxime Non-susceptible Streptococcus pneumoniae: Evidence and Challenges

Frontiers in Microbiology, 2022
The principal causative agent of acute bacterial meningitis (ABM) in children and the elderly is Streptococcus pneumoniae, with a widespread increase in penicillin resistance.
Rosemol Varghese, Soumya Basu, Ayyanraj Neeravi, Agilakumari Pragasam, V. Aravind, Richa Gupta, Angel Miraclin, Sudha Ramaiah, Anand Anbarasu, Balaji Veeraraghavan   +9 more
doaj   +1 more source

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a [PDF]

, 2014
Surfactant-mediated removal of proteins from biomembranes invariably results in partial or complete loss of function and disassembly of multi-protein complexes. We determined the capacity of styrene-co-maleic acid (SMA) co-polymer to remove components of
Dafforn, Timothy R, Foster, Simon J, Galley, Nicola F, Garcia-Lara, Jorge, Jamshad, Mohammed, Paulin, Sarah, Roper, David I, Rosado, Helena, Taylor, Peter W   +8 more
core   +3 more sources

Penicillin-binding proteins of Haemophilus ducreyi [PDF]

Antimicrobial Agents and Chemotherapy, 1989
The penicillin-binding protein (PBP) profile of Haemophilus ducreyi was determined by a whole-cell-labeling assay. Only two major PBPs, of molecular weights 90,000 (PBP 1) and 38,500 (PBP 2), were detected in six of eight strains studied. Competition binding experiments and the attendant morphological effects suggested that PBP 1 was either a ...
B C, Lee, L E, Bryan
openaire   +2 more sources