Results 61 to 70 of about 232,235 (310)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Characterization of the mitochondrial processing peptidase of Neurospora crassa [PDF]

, 1994
The mitochondrial processing peptidase (MPP) of Neurospora crassa is constituted by an alpha- and a beta-subunit. We have purified alpha-MPP after expression in Escherichia coli while beta-MPP was purified from mitochondria.
Arretz, Michael, Brunner, Michael, Guiard, Bernard, Neupert, Walter, Schneider, Helmut   +4 more
core  

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Proteomic analysis of human skin treated with larval schistosome peptidases reveals distinct invasion strategies among species of blood flukes. [PDF]

PLoS Neglected Tropical Diseases, 2011
Skin invasion is the initial step in infection of the human host by schistosome blood flukes. Schistosome larvae have the remarkable ability to overcome the physical and biochemical barriers present in skin in the absence of any mechanical trauma.
Jessica Ingram, Giselle Knudsen, K C Lim, Elizabeth Hansell, Judy Sakanari, James McKerrow   +5 more
doaj   +1 more source

Specific lid-base contacts in the 26s proteasome control the conformational switching required for substrate degradation. [PDF]

, 2019
The 26S proteasome is essential for proteostasis and the regulation of vital processes through ATP-dependent degradation of ubiquitinated substrates. To accomplish the multi-step degradation process, the proteasomes regulatory particle, consisting of lid
Aufderheide, Bard, Bard, Bard, Bashore, Beckwith, Dambacher, de la Peña, Ding, Dong, Eisele, Elsasser, Finley, Glickman, Glickman, Goldstein, Groll, Hershko, Kachroo, Lander, Lasker, Longtine, Matyskiela, Myers, Nemec, Peth, Prakash, Rubin, Smith, Sone, Tomko, Unverdorben, Verma, VerPlank, VerPlank, Wehmer, Wendler, Wenzel, Worden, Worden, Yao, Zhu, Śledź   +42 more
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Brush border digestion : development of a physiologically relevant in vitro model : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Physiology at Massey University, Manawatū, New Zealand [PDF]

, 2018
Chapter Two has, in part, been published in the Springer publication, Reviews of Physiology, Biochemistry and Pharmacology, but the Author retains the right to publish the material in any collection consisting of the Author's own works: Hooton, D ...
Hooton, Diane Frances
core  

Green Production of Anionic Surfactant Obtained from Pea Protein [PDF]

, 2011
A pea protein isolate was hydrolyzed by a double enzyme treatment method in order to obtain short peptide sequences used as raw materials to produce lipopeptides-based surfactants.
A Clemente, A Tsoukala, A Villanueva, AF Dexter, Bénédicte Portet, C Megias, C Rondel, Caroline Rondel, CN Mulligan, D Kitamoto, EA Pena-Ramos, FM Netto, Françoise Silvestre, H Frister, H Schägger, Isabelle Alric, J Vioque, Jean-François Blanco, JS Hamada, JY Lee, K Pommer, K Yasamatsu, LA Graaf De, LM Humiski, M Dall Aaslyng, M Karamac, M Le Gall, MC Marquez, MPC Silvestre, MR Infante, P Anastas, PM Nielsen, R Amarowicz, R Sanchez-Vioque, R Sanchez-Vioque, R Sinha, RT Swank, S Jung, TS Padmashree, UK Laemmli, Zéphirin Mouloungui   +40 more
core   +2 more sources

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Mitochondrial processing peptidases

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2002
Three peptidases are responsible for the proteolytic processing of both nuclearly and mitochondrially encoded precursor polypeptides targeted to the various subcompartments of the mitochondria. Mitochondrial processing peptidase (MPP) cleaves the vast majority of mitochondrial proteins, while inner membrane peptidase (IMP) and mitochondrial ...
Gakh, Oleksandr, Cavadini, Patrizia, Isaya, Grazia   +2 more
openaire   +2 more sources