Results 1 to 10 of about 319,110 (303)

Leader peptide removal in lasso peptide biosynthesis based on penultimate isoleucine residue [PDF]

Frontiers in Microbiology, 2023
Lasso peptides are ribosomally synthesized peptides that undergo post-translational modifications including leader peptide removal by B (or the segregated B1 and B2) proteins and core peptide macrolactamization by C proteins to form a unique lariat ...
Yuwei Duan, Weijing Niu, Linlin Pang, Da-Shuai Mu, Da-Shuai Mu, Zong-Jun Du, Zong-Jun Du, Youming Zhang, Youming Zhang, Xiaoying Bian, Guannan Zhong, Guannan Zhong   +11 more
doaj   +2 more sources

S9 protease WprP2 catalyzes uniform cleavage on the precursor peptide in RiPP biosynthesis [PDF]

Communications Chemistry
Serine proteases in ribosomally synthesized and post-translationally modified peptides (RiPPs) catalyze the cleavage on the precursor peptides in the biosynthesis of RiPP natural products. Here, we identified an uncharacterized serine protease WprP2 from
Jabal Rahmat Haedar, Abujunaid Habib Khan, Suze Ma, Stefano Donadio, Chin-Soon Phan   +4 more
doaj   +2 more sources

Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi. [PDF]

Methods Mol Biol, 2016
Filamentous fungi are historically known as rich sources for production of biologically active natural products, so-called secondary metabolites. One particularly pharmaceutically relevant chemical group of secondary metabolites is the nonribosomal peptides synthesized by nonribosomal peptide synthetases (NRPSs).
Soukup AA, Keller NP, Wiemann P.
europepmc   +4 more sources

Enzymatic activities of proteins encoded by PmurB, PmurC, and PmurE involved in methanogen pseudomurein biosynthesis [PDF]

Frontiers in Microbiology
Pseudomurein, a glycan polymer present in the cell wall, is found in the orders Methanobacteriales and Methanopyrales. It possesses glycan units and peptide chains similar to those of bacterial peptidoglycan (murein).
Guihong Cha, Guihong Cha, Zhenli Lai, Shuxin Wang, Qing Yang, Pengyan Zhao, Yi Chen, Wei Han, Liping Bai, Liping Bai   +9 more
doaj   +2 more sources

Thermostable chaperone-based polypeptide biosynthesis: Enfuvirtide model product quality and protocol-related impurities.

PLoS ONE, 2023
Large peptide biosynthesis is a valuable alternative to conventional chemical synthesis. Enfuvirtide, the largest therapeutic peptide used in HIV infection treatment, was synthesized in our thermostable chaperone-based peptide biosynthesis system and ...
Vladimir Zenin, Andrey Tsedilin, Maria Yurkova, Andrey Siniavin, Alexey Fedorov   +4 more
doaj   +3 more sources

Expression and Activity Identification of Recombinant Antihypertensive Peptide (Angiotensin I-Converting Enzyme Inhibitory Peptide) [PDF]

Shipin Kexue, 2023
In this study, the antihypertensive peptide WQVLPNAVPAK was selected for the synthesis of the gene encoding the hexameric peptide angiotensin I-converting enzyme inhibitory peptide (ACEIP) based on the codon preference of Escherichia coli.
DI Nana, JIANG Bo, ZHANG Ran, CAO Hongzhen, CHEN Jingjing, ZHANG Tao
doaj   +1 more source

The Surfactin-Like Lipopeptides From Bacillus spp.: Natural Biodiversity and Synthetic Biology for a Broader Application Range

Frontiers in Bioengineering and Biotechnology, 2021
Surfactin is a lipoheptapeptide produced by several Bacillus species and identified for the first time in 1969. At first, the biosynthesis of this remarkable biosurfactant was described in this review.
Ariane Théatre, Carolina Cano-Prieto, Marco Bartolini, Yoann Laurin, Yoann Laurin, Magali Deleu, Joachim Niehren, Tarik Fida, Saïcha Gerbinet, Mohammad Alanjary, Marnix H. Medema, Angélique Léonard, Laurence Lins, Ana Arabolaza, Hugo Gramajo, Harald Gross, Philippe Jacques   +16 more
doaj   +1 more source

Enfuvirtide biosynthesis in thermostable chaperone-based fusion

Biotechnology Reports, 2022
Synthetic peptides are in high demand as biologically active substances. Solid phase synthesis is the primary method of peptide production. However, it has drawbacks: large amount of chemical waste and rapid increase in price with peptide length ...
Vladimir Zenin, Maria Yurkova, Andrey Tsedilin, Alexey Fedorov   +3 more
doaj   +1 more source

Isolation and Analysis of the Nisin Biosynthesis Complex NisBTC: further Insights into Their Cooperative Action

mBio, 2021
Lantibiotics are ribosomally synthesized and posttranslationally modified peptide antibiotics. Although the membrane-associated lantibiotic biosynthesis machinery has long been proposed to exist, the isolation of such a complex has not been reported yet,
Jingqi Chen, Oscar P. Kuipers
doaj   +1 more source

A Cysteine-Reloading Process Initiating the Biosynthesis of the Bicyclic Scaffold of Dithiolopyrrolones

Antibiotics, 2023
Dithiolopyrrolone antibiotics are well known for their outstanding biological activities, and their biosynthesis has been studied vigorously. However, the biosynthesis mechanism of the characteristic bicyclic scaffold is still unknown after years of ...
Yan Chen, Yanqin Tu, Tingyu Pan, Zixin Deng, Lian Duan   +4 more
doaj   +1 more source