Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support [PDF]
BMC Biotechnology, 2005 Background Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.
Leclerc Mario +7 more
doaj +6 more sources
PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor [PDF]
Biosensors, 2016 A PCR-free, optics-free device is used for the detection of Escherichia coli (E. coli) 16S rRNA at 10 fM, which corresponds to ~100–1000 colony forming units/mL (CFU/mL) depending on cellular rRNA levels.
Leyla Esfandiari +7 more
doaj +3 more sources
Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2). [PDF]
PLoS ONE, 2013 We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production.
Gabriel C Uguru +6 more
doaj +6 more sources
Identification of a cardiac specific protein transduction domain by in vivo biopanning using a M13 phage peptide display library in mice. [PDF]
PLoS ONE, 2010 BACKGROUND: A peptide able to transduce cardiac tissue specifically, delivering cargoes to the heart, would be of significant therapeutic potential for delivery of small molecules, proteins and nucleic acids.
Maliha Zahid +5 more
doaj +7 more sources
Supporting data for the characterization of PNA–DNA four-way junctions [PDF]
Data in Brief, 2015 Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair.
Douglas Iverson +5 more
doaj +3 more sources
Peptide nucleic acid-zirconium coordination nanoparticles
Scientific Reports, 2023 Ideal drug carriers feature a high loading capacity to minimize the exposure of patients with excessive, inactive carrier materials. The highest imaginable loading capacity could be achieved by nanocarriers, which are assembled from the therapeutic cargo
Özgür Öztürk +6 more
doaj +1 more source
Nucleic Acid Carriers Based on Precise Polymer Conjugates [PDF]
, 2011 Polymer polydispersity, random conjugation of functional groups, and poorly understood structure–activity relationships have constantly hampered progress in the development of nucleic acid carriers.
Troiber, Christina, Wagner, Ernst
core +4 more sources
Investigation of the Characteristics of NLS-PNA: Influence of NLS Location on Invasion Efficiency
Applied Sciences, 2020 Peptide nucleic acid can recognise sequences in double-stranded DNA (dsDNA) through the formation of a double-duplex invasion complex. This double-duplex invasion is a promising method for the recognition of dsDNA in cellula because peptide nucleic acid (
Yuichiro Aiba +3 more
doaj +1 more source
Applied Sciences, 2022 Peptide nucleic acid (PNA) is an analog of natural nucleic acids, where the sugar-phosphate backbone of DNA is replaced by an electrostatically neutral N-(2-aminoethyl)glycine backbone.
Yuichiro Aiba +2 more
doaj +1 more source
An Elisa-Based Platform for Rapid Identification of Structure-Dependent Nucleic Acid-Protein Interactions Detects Novel DNA Triplex Interactors [PDF]
, 2022 Unusual nucleic acid structures play vital roles as intermediates in many cellular processes and, in the case of peptide nucleic acid (PNA)-mediated triplexes, are leveraged as tools for therapeutic gene editing.
Balasubramanian, Nanda +4 more
core +2 more sources