Determining bacteriophage endopeptidase activity using either fluorophore-quencher labeled peptides combined with liquid chromatography-mass spectrometry (LC-MS) or Förster resonance energy transfer (FRET) assays. [PDF]
PLoS ONE, 2017 The necessity of identifying novel methods to combat infections caused by antibiotic resistant bacteria is increasing each year. Recent advancements in the development of peptidoglycan hydrolases (e.g. lysins) from bacterial viruses (bacteriophages) have
Rolf Lood +2 more
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Relating the metatranscriptome and metagenome of the human gut [PDF]
, 2014 Although the composition of the human microbiome is now wellstudied, the microbiota’s \u3e8 million genes and their regulation remain largely uncharacterized.
Boylan, Matthew R. +13 more
core +2 more sources
eLife, 2014 Bacteria have to avoid recognition by the host immune system in order to establish a successful infection. Peptidoglycan, the principal constituent of virtually all bacterial surfaces, is a specific molecular signature recognized by dedicated host ...
Magda Luciana Atilano +9 more
doaj +1 more source
Cell wall elongation mode in Gram-negative bacteria is determined by peptidoglycan architecture [PDF]
, 2013 Cellular integrity and morphology of most bacteria is maintained by cell wall peptidoglycan, the target of antibiotics essential in modern healthcare.
Cadby, A. +4 more
core +1 more source
Anchoring of proteins to lactic acid bacteria [PDF]
, 1999 The anchoring of proteins to the cell surface of lactic acid bacteria (LAB) using genetic techniques is an exciting and emerging research area that holds great promise for a wide variety of biotechnological applications. This paper reviews five different
A Hoick +66 more
core +1 more source
A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens [PDF]
Science, 2016 The intestinal microbiome modulates host susceptibility to enteric pathogens, but the specific protective factors and mechanisms of individual bacterial species are not fully characterized. We show that secreted antigen A (SagA) from Enterococcus faecium is sufficient to protect Caenorhabditis elegans
Kavita J. Rangan +7 more
openaire +3 more sources
Acd, a peptidoglycan hydrolase of Clostridium difficile with N-acetylglucosaminidase activity [PDF]
Microbiology, 2005 A gene encoding a putative peptidoglycan hydrolase was identified by sequence similarity searching in the Clostridium difficile 630 genome sequence, and the corresponding protein, named Acd (autolysin of C. difficile) was expressed in Escherichia coli. The deduced amino acid sequence of Acd shows a modular structure with two main domains: an N-terminal
Anne Dhalluin +7 more
openalex +4 more sources
Binding Mechanism of the Peptidoglycan Hydrolase Acm2: Low Affinity, Broad Specificity [PDF]
Biophysical Journal, 2013 Peptidoglycan hydrolases are bacterial secreted enzymes that cleave covalent bonds in the cell-wall peptidoglycan, thereby fulfilling major physiological functions during cell growth and division. Although the molecular structure and functional roles of these enzymes have been widely studied, the molecular details underlying their interaction with ...
Pascal Hols +6 more
openaire +3 more sources
Frontiers in Microbiology, 2017 The antimicrobial activity of the chemokine CXCL10 against vegetative cells of Bacillus anthracis occurs via both bacterial FtsE/X-dependent and-independent pathways.
Molly A. Hughes +6 more
doaj +1 more source
Sonic Hedgehog Is a Member of the Hh/DD-Peptidase Family That Spans the Eukaryotic and Bacterial Domains of Life. [PDF]
, 2018 Sonic Hedgehog (Shh) coordinates Zn2+ in a manner that resembles that of peptidases. The ability of Shh to undergo autoproteolytic processing is impaired in mutants that affect the Zn2+ coordination, while mutating residues essential for catalytic ...
Roelink, Henk
core +3 more sources