Peptidyl transferase: ancient and exiguous [PDF]
Chemistry & Biology, 2000 The finding that the universal ribosomal peptidyl transferase is an RNA enzyme casts new light on its ancient origins, on the use of transition state analogues for ribozymes, and on the role of selection-amplification in studies of molecular evolution.
Yarus, Michael, Welch, Mark
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Chemical synthesis and biological activities of novel pleuromutilin derivatives with substituted amino moiety. [PDF]
PLoS ONE, 2013 Novel pleuromutilin derivatives designed based on the structure of valnemulin were synthesized and evaluated for their in vitro antibacterial activities. These pleuromutilin derivatives with substituted amino moiety exhibited excellent activities against
Ruofeng Shang +6 more
doaj +1 more source
The insect pathogen Serratia marcescens Db10 uses a hybrid non-ribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin [PDF]
, 2012 There is a continuing need to discover new bioactive natural products, such as antibiotics, in genetically-amenable micro-organisms. We observed that the enteric insect pathogen, Serratia marcescens Db10, produced a diffusible compound that inhibited the
Amy J. Gerc +5 more
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Computational and Structural Biotechnology Journal, 2023 The central function of the large subunit of the ribosome is to catalyze peptide bond formation. This biochemical reaction is conducted at the peptidyl transferase center (PTC).
Marc Joiret +4 more
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Mapping of Complete Set of Ribose and Base Modifications of Yeast rRNA by RP-HPLC and Mung Bean Nuclease Assay. [PDF]
PLoS ONE, 2016 Ribosomes are large ribonucleoprotein complexes that are fundamental for protein synthesis. Ribosomes are ribozymes because their catalytic functions such as peptidyl transferase and peptidyl-tRNA hydrolysis depend on the rRNA.
Jun Yang +5 more
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Liver transplantation for type IV glycogen storage disease [PDF]
, 1991 TYPE IV glycogen storage disease is a rare autosomal recessive disorder (also called Andersen's disease1 or amylopectinosis) in which the activity of branching enzyme alpha-1, 4-glucan: alpha-1, 4-glucan 6-glucosyltransferase is deficient in the liver as
Andreas Tzakis +17 more
core +1 more source
Ribosomal history reveals origins of modern protein synthesis. [PDF]
PLoS ONE, 2012 The origin and evolution of the ribosome is central to our understanding of the cellular world. Most hypotheses posit that the ribosome originated in the peptidyl transferase center of the large ribosomal subunit.
Ajith Harish, Gustavo Caetano-Anollés
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Visualizing formation of the active site in the mitochondrial ribosome
eLife, 2021 Ribosome assembly is an essential and conserved process that is regulated at each step by specific factors. Using cryo-electron microscopy (cryo-EM), we visualize the formation of the conserved peptidyl transferase center (PTC) of the human mitochondrial
Viswanathan Chandrasekaran +6 more
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Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release. [PDF]
, 2018 Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A site and is mediated by class I release factors (RFs).
Clementi, Nina +11 more
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Extensive ribosome and RF2 rearrangements during translation termination
eLife, 2019 Protein synthesis ends when a ribosome reaches an mRNA stop codon. Release factors (RFs) decode the stop codon, hydrolyze peptidyl-tRNA to release the nascent protein, and then dissociate to allow ribosome recycling.
Egor Svidritskiy +4 more
doaj +1 more source