Interaction of Pleuromutilin Derivatives with the Ribosomal Peptidyl Transferase Center [PDF]
Antimicrobial Agents and Chemotherapy, 2006 ABSTRACTTiamulin is a pleuromutilin antibiotic that is used in veterinary medicine. The recently published crystal structure of a tiamulin-50S ribosomal subunit complex provides detailed information about how this drug targets the peptidyl transferase center of the ribosome.
Long, K. S. +3 more
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Nuclear translation or nuclear peptidyl transferase? [PDF]
Nucleus, 2012 It is widely accepted that protein synthesis occurs in the cytoplasms of eukaryotic cells, but some investigators believe that it also occurs in the nucleus. In spite of experiments performed in several labs over many years, the issue of nuclear translation remains unresolved.
James, Dahlberg, Elsebet, Lund
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Modulating the activity of the peptidyl transferase center of the ribosome [PDF]
RNA, 2008 The peptidyl transferase (PT) center of the ribosome catalyzes two nucleophilic reactions, peptide bond formation between aminoacylated tRNA substrates and, together with release factor, peptide release. Structure and function of the PT center are modulated by binding of aminoacyl-tRNA or release factor, thus providing the basis for the specificity of ...
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4 -Phosphopantetheine Transfer in Bacillus subtilis And Pseudomonas aeruginosa as Well as Preparatory Work for Directed Protein Evolution of Adenylation Domains of Nonribosomal Peptide Synthetases [PDF]
, 2011 Carrier Proteine (CP) werden in unterschiedlichen prokaryontischen und eukaryontischen Synthesesystemen als Transporteinheiten für die Syntheseintermediate eingesetzt.
Finking, Robert (128510439)
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Synthèse d'analogues d'aminoacyl-ARNt pour l'étude et l'inhibition de Fem-transférases, nouvelles cibles pour le développement d'antibactériens [PDF]
, 2016 Because of their involvement in numerous biological processes, aminoacyl-tRNA are a topic of increasing interest in RNAbiology. Among their roles, they participate in the biosynthesis of the bacterial cell-wall in many Gram-positive bacteria assubstrates
Sakkas, Nicolas
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Substrate Specificity of Escherichia coli Peptidyl‐Transferase [PDF]
European Journal of Biochemistry, 1970 The kinetics of the puromycin interaction with different ribosomal bound peptidyl‐tRNAs were studied. The rates of the puromycin reaction with AA‐Phe‐tRNA and AA2‐Phe‐tRNA using three different amino acids (AA) were compared. At 4° all the three dipeptidyl‐tRNAsPhe reacted considerably slower than the three tripeptidyl‐tRNAsPhe. Acetyl‐Phe‐tRNA reacted
A, Panet, N, de Groot, Y, Lapidot
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RNA aptamers to the peptidyl transferase inhibitor chloramphenicol
Chemistry & Biology, 1997 The problem of how macromolecules adopt specific shapes to recognize small molecules in their environment is readily addressed through in vitro selections (the SELEX protocol). RNA-antibiotic interactions are particularly attractive systems for study because they provide an opportunity to expand our understanding of molecular recognition by RNA and to ...
Burke, Donald H. +5 more
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Effect of cytidine‐5′‐monophosphate on peptidyl transferase activity [PDF]
FEBS Letters, 1975 The transfer reaction with pA-fMet as a donor substrate is strongly stimulated by CMP, whereas the transfer reaction with CpApCpCpA-acLeu as a donor substrate is inhibited by CMP. These results indicate that the donor site of peptidyl transferase contains specific binding sites for the terminal adenosine and for the cytidylic acid residue in the ...
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The ribosome as a versatile catalyst: reactions at the peptidyl transferase center [PDF]
Current Opinion in Structural Biology, 2013 In all contemporary organisms, the active site of the ribosome--the peptidyl transferase center--catalyzes two distinct reactions, peptide bond formation between peptidyl-tRNA and aminoacyl-tRNA as well as the hydrolysis of peptidyl-tRNA with the help of a release factor. However, when provided with appropriate substrates, ribosomes can also catalyze a
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Characterization of the Streptococcolytic enzyme zoocin A and its resistance factor, zif [PDF]
, 2010 Zoocin A is a bacteriolytic enzyme produced by Streptococcus equi subsp. zooepidemicus 4881 that is able to hydrolyze the cell walls of some streptococcal species. The site of action on peptidoglycan by zoocin A was previously unknown.
Churchill, Perry F. +7 more
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