Results 101 to 110 of about 70,536 (266)

Optimization of seminal extracellular vesicle isolation protocols for cancer biomarker analysis using proteomic profiling

Molecular Oncology, EarlyView.
This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan, Beatriz Martín‐Gracia, Eva Bassols‐Citores, Krizia Sagini, Maria K. Andersen, Tuula A. Nyman, May‐Britt Tessem, Morten B. Rye, Alicia Llorente   +8 more
wiley   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer

Molecular Oncology, EarlyView.
New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau, Daniel Pincher, Clifford Young, Nicholas Chappell, Noor A. Lokman, Parul Mittal, Leigh Donnellan, Martin K. Oehler, Doug A. Brooks, Peter Hoffmann   +9 more
wiley   +1 more source

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

PERFORMANCES DES BREBIS ROMANOV SOUMISES A UN RYTHME ACCÉLÉRÉ DE REPRODUCTION [PDF]

Annales de Zootechnie, 1973
Tchamitchian, L., Ricordeau, G., Lefèvre, C., Desvignes, A., Lajous, D.   +4 more
openaire   +2 more sources

Performances de reproduction et de production laitière des vaches Pie-Noires au Maroc

Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux, 1986
L'étude a porté sur 1827 résultats du contrôle laitier effectué sur 807 vaches Frisonne-Holstein élevées dans 24 étables localisées dans 4 régions agricoles. La moyenne de l'âge au premier vêlage est de 29,5 mois, l'intervalle vêlage-fécondation est de 139,4 jours et l'intervalle entre deux vêlages est de 411,2 jours.
I. Boujenane, Maty Ba
openaire   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Definición de conceptos jurídicos vs. Listado de enfermedades susceptibles como soluciones respecto al uso del diagnóstico genético preimplantacional.

Bioderecho.es, 2018
Resumen: La Ley española 14/2006, de 26 de mayo, sobre técnicas de reproducción humana asistida, acepta la realización de un diagnóstico genético preimplantacional (DGP) cuando se trata de evitar una enfermedad genética grave, de aparición precoz y sin ...
Joaquín Jiménez González
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Interpretação - reprodução musical - teoria da performance: reunindo-se os elementos para uma reformulação conceitual da(s) prática(s) interpretativa(s) Interpretation - musical reproduction - theory of performance: bringing together the elements for a conceptual reform of the interpretative and the performance practice as a scholar discipline

Per Musi, 2012
Notam-se nas ciências humanas enormes dificuldades na definição e no emprego dos conceitos que designam fenômenos culturais. Isso vale também para a área da música, onde costuma se notar certa confusão quando se passa para o entendimento do que designam ...
Frank Michael Carlos Kuehn
doaj