Results 121 to 130 of about 73,546 (303)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Avaliação de bezerros de raças leiteiras em sistemas de criação com vaca-ama e com balde [PDF]
, 2004 Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Agroecossistemas.Os bovinos são animais sociais, que entre eles estabelecem uma ordem de dominância.
Dinon, Patrícia Silva de Lorenzi
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How does caloric stress affect reproduction?
, 2022 La presente revisión analiza algunos de los efectos del estrés calórico sobre la reproducción. El estrés se manifiesta por la incapacidad de un animal para hacer frente a su entorno, un fenómeno que a menudo se refleja en la pérdida del potencial ...
Vélez Marín, Miryam +1 more
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FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Social and biological reproduction of lower-income groups on the meseta central in costa rica [PDF]
, 1988 This thesis examines the social and biological reproduction of households in the sectores populares, ie low income groups, both rural and urban, in the Meseta Central, yields specific outcomes at household level: the social formation based, originally ...
Lopez-Casas, E, Lopez-Casas, Eugenia
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FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Rethinking the Box: Approaches to the Reality of Electronic Music Performance
This article extends certain aspects of the work presented in the Thinking Inside the Box Project, by exploring efficient software-based methods for improving sound reproduction within the concert hall. The key problems discussed are: (1) the detrimental
Harker, Alexander +1 more
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Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source