Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors [PDF]
STAR Protocols, 2022 Summary: Hydrogen sulfide (H2S) and downstream reactive sulfur species (RSS), including organic persulfides, protect bacterial cells against diverse oxidative stressors.
Joseph N. Fakhoury +2 more
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Redox Signaling Regulated by Cysteine Persulfide and Protein Polysulfidation
Molecules, 2016 For decades, reactive persulfide species including cysteine persulfide (CysSSH) have been known to exist endogenously in organisms. However, the physiological significance of endogenous persulfides remains poorly understood. That cystathionine β-synthase
Shingo Kasamatsu +2 more
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Enzymes that generate and regulate intracellular persulfides and polysulfides: mechanistic insights and inhibitors [PDF]
Frontiers in PhysiologyReactive sulfur species (RSS), which include various persulfides and polysulfides, are generated by multiple enzymes in vivo and play critical roles in mammalian physiological processes such as redox signaling, metabolic regulation, radical scavenging ...
Ko Hirabayashi +6 more
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A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems [PDF]
Science Advances, 2016 Hydrogen sulfide signaling involves persulfide formation at specific protein Cys residues. However, overcoming current methodological challenges in persulfide detection and elucidation of Cys regeneration mechanisms from persulfides are prerequisites for
Eva Doka +2 more
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Pseudomonas aeruginosa PA1006 is a persulfide-modified protein that is critical for molybdenum homeostasis. [PDF]
PLoS ONE, 2013 A companion manuscript revealed that deletion of the Pseudomonas aeruginosa (Pae) PA1006 gene caused pleiotropic defects in metabolism including a loss of all nitrate reductase activities, biofilm maturation, and virulence.
Gregory Tombline +8 more
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Acidity and nucleophilic reactivity of glutathione persulfide [PDF]
Journal of Biological Chemistry, 2020 Persulfides (RSSH/RSS2) participate in sulfur trafficking and metabolic processes, and are proposed to mediate the signaling effects of hydrogen sulfide (H2S). Despite their growing relevance, their chemical properties are poorly understood.
Dayana Benchoam +2 more
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ProPerDP: A Protein Persulfide Detection Protocol
Methods in Molecular Biology, 2019 Persulfide or polysulfide formation on Cys residues is emerging as an abundant protein posttranslational modification, with important regulatory functions.
Eva Doka +2 more
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Growth inhibitory factor/metallothionein-3 is a sulfane sulfur-binding protein [PDF]
eLifeCysteine-bound sulfane sulfur atoms in proteins have received much attention as key factors in cellular redox homeostasis. However, the role of sulfane sulfur in zinc regulation has been underinvestigated.
Yasuhiro Shinkai +12 more
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Redox Biology, 2023 Hydropersulfide and hydropolysulfide metabolites are increasingly important reactive sulfur species (RSS) regulating numerous cellular redox dependent functions.
Shafiul Alam +13 more
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Communicative & Integrative Biology, 2017 We recently identified the novel persulfide sensor SqrR that functions as a master regulator of sulfide-dependent gene expression in the purple photosynthetic bacterium Rhodobacter capsulatus. SqrR binds to the promoter regions of target genes to repress
Takayuki Shimizu, Shinji Masuda
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