Results 181 to 190 of about 2,565 (197)

Hybrid genes over-express pertactin from Bordetella pertussis

Vaccine, 1995
Pertactin is a surface adhesin of Bordetella pertussis which is produced in small quantities when expressed from the native prn promoter. Hybrid genes were constructed in which the prn promoter was replaced by either the fha or tox promoter. Recombinant B.
Graham E Jackson, David C Coleman
exaly   +3 more sources

Uncovering the folding mechanism of pertactin: A comparative study of isolated and vectorial folding

Biophysical Journal, 2023
Autotransporters are a large family of virulence factors found in Gram-negative bacteria that play important roles in their pathogenesis. The passenger domain of autotransporters is almost always composed of a large β-helix, with only a small portion of it being relevant to its virulence function.
Yui Tik Pang, Anthony J. Hazel, James C. Gumbart   +2 more
openaire   +2 more sources

Development of a Pertactin-Coated Beads Approach for Screening of Functional Monoclonal Antibodies

Journal of Pharmaceutical Sciences, 2020
Vaccine manufacturers have recently focused on the development of in vitro potency assays to promote 3R's strategy to replace animal testing. To be able to develop an in vitro potency assay, the immunological characteristics of the monoclonal antibodies used in the assay should be well understood as these antibodies likely reflect the biological ...
Liwei, He, Issaka, Yougbare, Beata, Gajewska, Jin, Su, Rachel, Leung, Ali, Azizi   +5 more
openaire   +2 more sources

The preparation of A purified immunogenic pertactin from B. pertussis.

The Egyptian journal of immunology, 2010
Pertactin, an outer membrane protein of Bordetella pertussis, was purified to apparent homogeneity. The purified pertactin was first extracted from the B. pertussis cells (strain 165) by heating for 1 h at 60 degrees C, followed by DEAE-Sepharose and Affi-Gel Blue chromatography.
E M, El-Kady, H M, Abu-Shady, H S, Tohamy, A F, Wahby   +3 more
openaire   +1 more source

P1768 Pertactin evaluation as immunogen in murine model

International Journal of Antimicrobial Agents, 2007
E. Rosales, A. Fernandez-Ramirez, G. Barcenas-Morales, J. Montaraz-Crespo   +3 more
openaire   +1 more source

Pertactin-Negative Bordetella pertussis Strains: Evidence for a Possible Selective Advantage

Clinical Infectious Diseases, 2015
Lucia Pawloski, Margaret Williams, Xuan Qin   +2 more
exaly  

Clinical Manifestations and Molecular Characterization of Pertactin-Deficient and Pertactin-ProducingBordetella pertussisin Children, Philadelphia 2007–2014

Clinical Infectious Diseases, 2017
Anne Marie Queenan, Long Sarah S
exaly  

Pertactin deficient Bordetella pertussis present a better fitness in mice immunized with an acellular pertussis vaccine

Vaccine, 2014
N Guiso
exaly  

Pertactin-Negative B pertussis and Vaccine Effectiveness

AAP Grand Rounds, 2015
openaire   +1 more source

Pertactin-negative variants of Bordetella pertussis in New York State: A retrospective analysis, 2004–2013

Molecular and Cellular Probes, 2014
Kimberlee A Musser, Shelley M Zansky
exaly