Results 71 to 80 of about 359,710 (297)

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Phage-displayed scFvs detect GI.1 VLPs.

, 2017
Phages displaying the novel single-chain antibodies detect GI.1 VLPs directly coated to the ELISA plate (A) and GI.1 VLPs captured by anti-NV polyclonal antibodies (B).
Wanzhi Huang (3243096), Timothy Palzkill (47788), Mary K. Estes (239563), Amy M. Hurwitz (3663292), Robert L. Atmar (294652), Baijun Kou (3663289)   +5 more
core   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption

Advanced Functional Materials, EarlyView.
Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy, Kyle B. Meerbott, Lorenzo Travaglini, Garima S. Dobhal, Artur Sawicki, Marc R. Knecht, Tiffany R. Walsh, Dominic J. Glover   +7 more
wiley   +1 more source

Characterization, antibacterial, and cytotoxic activities of silver nanoparticles using the whole biofilm layer as a macromolecule in biosynthesis

Scientific Reports
Recently, multi-drug resistant (MDR) bacteria are responsible for a large number of infectious diseases that can be life-threatening. Globally, new approaches are targeted to solve this essential issue.
Aghapy Yermans Yakoup, Azza G. Kamel, Yasmin Elbermawy, Abdallah S. Abdelsattar, Ayman El-Shibiny   +4 more
doaj   +1 more source

Comprehensive epidemiological profiling of poultry-derived Salmonella spp. in Shandong, China, 2019–2022: a longitudinal study of prevalence, antibiotic resistances, virulence factors and molecular characteristics

Frontiers in Microbiology
Salmonella spp., as a major foodborne pathogen, pose significant threats to public health globally and has been an important zoonotic contamination for poultry industry that should receive increasing attentions.
Lele Chen, Lele Chen, Yuxia Shi, Yuxia Shi, Minge Wang, Minge Wang, Yubao Li, Zhenshu Si, Zhenshu Si   +8 more
doaj   +1 more source

Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis

, 2021
Ligand-dependent biosensors are valuable tools for coupling the intracellular concentrations of small molecules to easily detectable readouts such as absorbance, fluorescence, or cell growth.
Ketaki Belsare (11427100), Bryan C. Dickinson (151937), Krysten A. Jones (1616632), Jared C. Lewis (1425370), Harrison M. Snodgrass (7540607)   +4 more
core   +1 more source

Nanoamphizymes: Self‐Assembled Amphiphilic Enzymatic Nanomaterials for High‐Performance Biocatalysis and Biosensing

Advanced Functional Materials, EarlyView.
An amphiphilic GOx nanoamphizyme was constructed by covalently introducing hydrophobic PPNI linkers onto GOx, enabling spontaneous self‐assembly into tunable nanoparticles. Mild crosslinking further stabilized the assemblies and enhanced catalytic performance and stability.
Yuan‐Hao Hsiao, Rajan Deepan Chakravarthy, Po‐Hsiang Chen, Abdelreheem Abdelfatah Saddik, Hsin‐Chieh Lin   +4 more
wiley   +1 more source

Large-genome phage vB_Eco_ZCEC15 targets gastrointestinal MDR E. coli: evidence from in vitro and Caco-2 cell models

BMC Microbiology
Background Bacterial resistance to traditional antibiotics is spreading at an alarming rate, threatening public health and various industrial applications.
Kareem Essam, Amira A. Mohamed, Salsabil Makky, Azza G. Kamel, Ayman El-Shibiny   +4 more
doaj   +1 more source

Intra-strain genomic microevolution and phage resistance in Pseudomonas aeruginosa PAO1 laboratory isolates

Microbiology Spectrum
Pseudomonas aeruginosa is a major opportunistic pathogen, and its laboratory reference strain, PAO1, is widely used in microbiological and genetic studies.
Ayaka Washizaki, Ajeng K. Pramono, Arata Sakiyama, Keiko Inaba-Hasegawa, Shoichi Mitsunaka, Hiroki Ando   +5 more
doaj   +1 more source