Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates [PDF]
MethodsX, 2021 Single molecule localization microscopy (SMLM) provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids but apart from the nuclear envelope the role of the nuclear lipids in the functional organization of the cell
Peter Hoboth +4 more
doaj +2 more sources
Quantitative structural characterization of phosphatidylinositol phosphates from biological samples [PDF]
Journal of Lipid Research, 2017 The aspects of cellular metabolism controlled by phosphatidylinositol phosphates (PtdInsPs) have been broadly expanded, and these phospholipids have drawn tremendous attention as pleiotropic signaling molecules.
Su Hee Kim +8 more
doaj +2 more sources
Acutely Modifying Phosphatidylinositol Phosphates on Endolysosomes Using Chemically Inducible Dimerization Systems [PDF]
Bio-ProtocolPhosphoinositides are rare membrane lipids that mediate cell signaling and membrane dynamics. PI(4)P and PI(3)P are two major phosphoinositides crucial for endolysosomal functions and dynamics, making them the lipids of interest in many studies.
Wei Sheng Yap, Peter Kim, Maxime Boutry
doaj +2 more sources
Generation of inositol polyphosphates through a phospholipase C-independent pathway involving carbohydrate and sphingolipid metabolism in Trypanosoma cruzi [PDF]
mBioInositol phosphates are involved in a myriad of biological roles and activities such as Ca2+ signaling, phosphate homeostasis, energy metabolism, and disease pathogenicity.
Mayara S. Bertolini +9 more
doaj +2 more sources
EFR3A, an Intriguing Gene, and Protein with a Scaffolding Function [PDF]
CellsThe EFR3 (Eighty-Five Requiring 3) protein and its homologs are rather poorly understood eukaryotic plasma membrane peripheral proteins. They belong to the armadillo-like family of superhelical proteins.
Magdalena Trybus +3 more
doaj +2 more sources
Crystal Structure of Human Myotubularin-Related Protein 1 Provides Insight into the Structural Basis of Substrate Specificity. [PDF]
PLoS ONE, 2016 Myotubularin-related protein 1 (MTMR1) is a phosphatase that belongs to the tyrosine/dual-specificity phosphatase superfamily. MTMR1 has been shown to use phosphatidylinositol 3-monophosphate (PI(3)P) and/or phosphatidylinositol 3,5-bisphosphate (PI(3,5 ...
Seoung Min Bong +9 more
doaj +1 more source
mBio, 2022 Phosphatidylinositol phosphates are key phospholipids with a range of regulatory roles, including membrane trafficking and cell polarity. Phosphatidylinositol-4-phosphate [PI(4)P] at the Golgi apparatus is required for the budding-to-filamentous-growth ...
Rocio Garcia-Rodas +7 more
doaj +1 more source
A tale of two inositol trisphosphates. [PDF]
, 2016 Between spring 1982 and autumn 1984 the physiological role of Ins(1,4,5)P3 as a calcium-mobilizing second messenger was first suggested and then experimentally established.
Irvine, Robin
core +4 more sources
Lipid Polarization during Cytokinesis
Cells, 2022 The plasma membrane of eukaryotic cells is composed of a large number of lipid species that are laterally segregated into functional domains as well as asymmetrically distributed between the outer and inner leaflets.
Govind Kunduri +2 more
doaj +1 more source
Optimization of chromatographic buffer conditions for the simultaneous analysis of phosphatidylinositol and phosphatidylinositol phosphate species in canola. [PDF]
, 2023 The phosphatidylinositols and phosphatidylinositol phosphates are a set of closely related lipids known to influence various cellular functions. Irregular distributions of these molecules have been correlated with the development and progression of ...
Bishop, DP, Gertner, DS, Padula, MP
core +1 more source