Results 41 to 50 of about 28,180 (258)

Extraction and Measurement the Activities of Cytosolic Phosphoenolpyruvate Carboxykinase (PEPCK) and Plastidic NADP-dependent Malic Enzyme (ME) on Tomato (Solanum lycopersicum)

Bio-Protocol, 2014
A recent study demonstrated that cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and NADP-malic enzyme (NADP-ME) have an important role in malate metabolism during fruit ripening (Osorio et al., 2013). PEPCK catalyze the ATP-dependent decarboxylation
Sonia Osorio, José Vallarino, Marek Szecowka, Shai Ufaz, Vered Tzin, Ruthie Angelovici, Gad Galili, Fayezeh Aarabi   +7 more
doaj   +1 more source

Minimizing acetate formation in E. coli fermentations [PDF]

, 2007
Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product.
De Maeseneire, Sofie, De Mey, Marjan, Soetaert, Wim, Vandamme, Erick   +3 more
core   +2 more sources

Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli

Journal of Hebei University of Science and Technology, 2019
In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show
Xiaoliang HE, Zhu2 LI, Liqiang2 LIANG, Wanli MENG, Lantian MIAO, Yaozu SUN, Hongguang2 CAI   +6 more
doaj   +1 more source

Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation

Nature Communications, 2017
Gluconeogenesis is downregulated in hepatocellular carcinoma. Here, the authors show that nuclear receptor Nur77 acts as a tumour suppressor sustaining gluconeogenesis by enhancing phosphoenolpyruvate carboxykinase (PEPCK1) stability via regulating its ...
Xue-li Bian, Hang-zi Chen, Peng-bo Yang, Ying-ping Li, Fen-na Zhang, Jia-yuan Zhang, Wei-jia Wang, Wen-xiu Zhao, Sheng Zhang, Qi-tao Chen, Yu Zheng, Xiao-yu Sun, Xiao-min Wang, Kun-Yi Chien, Qiao Wu   +14 more
doaj   +1 more source

Effect of pyruvate kinase gene deletion on the physiology of Corynebacterium glutamicum ATCC13032 under biotin-sufficient non-glutamate-producing conditions: Enhanced biomass production

Metabolic Engineering Communications, 2015
The effect of pyruvate kinase gene (pyk) deletion on the physiology of Corynebacterium glutamicum ATCC13032 was investigated under biotin-sufficient, non-glutamate-producing conditions.
Kazunori Sawada, Masaru Wada, Takuya Hagiwara, Susumu Zen-in, Keita Imai, Atsushi Yokota   +5 more
doaj   +1 more source

Changes in the microsomal proteome of tomato fruit during ripening [PDF]

, 2019
The variations in the membrane proteome of tomato fruit pericarp during ripening have been investigated by mass spectrometry-based label-free proteomics.
De Lorenzo, Giulia, Fabbri, Claudia, Licursi, Valerio, Mattei, Benedetta, Negri, Rodolfo, Pontiggia, Daniela, Spinelli, Francesco   +6 more
core   +1 more source

Lack of association between PCK1 polymorphisms and obesity, physical activity, and fitness in European Youth Heart Study (EYHS) [PDF]

, 2010
Phosphoenolpyruvate carboxykinase-1 (PCK1) is the rate-limiting enzyme in the hepatic gluconeogenic pathway. Studies have shown that overexpression of Pck1 in mice results in obesity-related traits and higher levels of physical activity (PA).
Anders Grontved, Beale, Brage, Brito, Cao, Chang, Chang, Cole, Cole, Ekelund, Ekelund, Franckhauser, Franckhauser, Gloria-Bottini, Goodpaster, Gutin, Hakimi, Hansen, Heard-Costa, Kang, Karani S. Vimaleswaran, Kristensen, Lane, Lee, Lindgren, Ludwig, Meyre, Nicholas J. Wareham, Oberkofler, Paul W. Franks, Rajas, Rees, Riddoch, Romeo, Rosella, Ruth J.F. Loos, Sardinha, Shin, Soren Brage, Tanner, Thorleifsson, Ulf Ekelund, Vimaleswaran, Wegner, Willer, Yu   +45 more
core   +1 more source

Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells[S]

Journal of Lipid Research, 2011
Previously, we have shown that Pck1 expression in mammary gland adipocytes and white adipose tissue maintains triglyceride stores through glyceroneogenesis, and these lipids were used for synthesis of milk triglycerides during lactation.
Chang-Wen Hsieh, Charles Huang, Ilya Bederman, Jianqi Yang, Michelle Beidelschies, Maria Hatzoglou, Michelle Puchowicz, Colleen M. Croniger   +7 more
doaj   +1 more source

Association of PCK1 with Body Mass Index and Other Metabolic Features in Patients With Psychotropic Treatments. [PDF]

, 2015
Weight gain is a major health problem among psychiatric populations. It implicates several receptors and hormones involved in energy balance and metabolism.
Aubry, J.M., Castelao, E., Choong, E., Conus, P., Delacrétaz, A., Eap, C.B., Gholamrezaee, M., Kutalik, Z., Magistretti, P., Preisig, M., Quteineh, L., Saigi-Morgui, N., Vandenberghe, F., Vollenweider, P., von Gunten, A., Waeber, G.   +15 more
core   +2 more sources

A Reactive Cysteine in Avian Liver Phosphoenolpyruvate Carboxykinase [PDF]

Journal of Biological Chemistry, 1989
The modification of avian phosphoenolpyruvate carboxykinase by a variety of sulfhydryl reagents leads to inhibition. The inhibition is related to the loss of 1 highly reactive cysteine residue of the 13 cysteines present in the enzyme. Inhibition by reagents which yield a mixed disulfide was rapidly reversed by thiols.
A L Makinen, Thomas Nowak
openaire   +2 more sources